2015
DOI: 10.4252/wjsc.v7.i1.223
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Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction

Abstract: AIM:To investigate adenoviral transduction in mesenchymal stem cells (MSCs) and effects on stemness in vitro and function as a cell therapy in vivo . METHODS:Bone marrow-derived adult and fetal MSC were isolated from an equine source and expanded in monolayer tissue culture. Polyethylenimine (PEI)-mediated transfection of pcDNA3-eGFP or adenoviral transduction of green fluorescent protein (GFP) was evaluated in fetal MSCs. Adenoviral-mediated transduction was chosen for subsequent experiments. All experiments … Show more

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Cited by 8 publications
(3 citation statements)
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References 25 publications
(32 reference statements)
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“…However, the stem cells have poor differentiation and a poor survival rate following their transplantation in vivo that has limited their regenerative potential [ 26 ]. Although the proliferative and differentiation capacities of hFMSCs can be retained and their osteogenic potentials may be enhanced through gene modulation [ 25 , 27 ], the genetic modification procedure is rather complicated and further studies are still needed before their clinical application. In contrast, cell-free secretome harvested from the hFMSC conditioned medium centrifugation is an easy and cost-effective procedure.…”
Section: Discussionmentioning
confidence: 99%
“…However, the stem cells have poor differentiation and a poor survival rate following their transplantation in vivo that has limited their regenerative potential [ 26 ]. Although the proliferative and differentiation capacities of hFMSCs can be retained and their osteogenic potentials may be enhanced through gene modulation [ 25 , 27 ], the genetic modification procedure is rather complicated and further studies are still needed before their clinical application. In contrast, cell-free secretome harvested from the hFMSC conditioned medium centrifugation is an easy and cost-effective procedure.…”
Section: Discussionmentioning
confidence: 99%
“…[31][32][33][34][35][36][37][38][39] Using viral vectors to insert genes into MSCs is a high transduction efficiency approach that has the potential to induce off-target effects owing to insertional mutagenesis. 32,35,40,41 Viral systems are also limited by relatively small transgene cargo capacity, high production cost, difficulties in production and scale-up, and adverse immune reactions. There are advantages and disadvantages to all known viral vectors, with the selection of an appropriate vector being dependent upon transduction rates and the desired duration of treatment and target gene expression.…”
Section: Vectors Used For Gf Overexpression In Mscsmentioning
confidence: 99%
“…Several studies involving cell therapy in osteoinduction processes have been conducted (Cuenca-López et al,2014;Dave et al, 2014;Santiago-Torres et al, 2015), but, experimental models have been restricted to rats, mice, sheep, dogs, and human cell culture; nonhuman primates have rarely been used in bone healing studies and have not focused on stem cells (Ripamonti et al, 2008;Emerton et al, 2011).…”
Section: Introductionmentioning
confidence: 99%