Fetal Surgery

Kunisaki, Shaun M.; Jennings, Russell W.

doi:10.1017/cbo9780511580987.022

Cited by 1 publication

(2 citation statements)

References 149 publications

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“…In one study comparing SIS and Alloderm with and without cells, no differences were observed in the amount of elastin (Fuchs et al, 2004). In another study comparing acellular grafts with constructs with fetal myoblasts or engineered tendon tissue, the elastin content was found to be higher in the latter (Kunisaki et al, 2006). However, histological localisation of the elastin was not reported.…”

Section: Discussionmentioning

confidence: 98%

“…Peritoneum and/or pleura have been reported to form on lyophilised dura (Rodgers et al, 1981), Dacron grafts (Dalton et al, 1966), and SIS and acellular dermis (Dalla Vecchia et al, 1999) in various animal models of diaphragmatic hernia. Only two studies have reported the presence of elastin in a diaphragmatic hernia animal model (Fuchs et al, 2004;Kunisaki et al, 2006). In one study comparing SIS and Alloderm with and without cells, no differences were observed in the amount of elastin (Fuchs et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

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Repair of surgically created diaphragmatic defect in rat with use of a crosslinked porous collagen scaffold

Brouwer

Daamen

Reijnen

et al. 2012

J Tissue Eng Regen Med

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Large defects in congenital diaphragmatic hernia are closed by patch repair, which is associated with a high complication risk and reherniation rate. New treatment modalities are warranted. We evaluated the feasibility of using an acellular biodegradable collagen bioscaffold for a regenerative medicine approach to close a surgically created diaphragmatic defect in a rat model. Scaffold degradation, cellular ingrowth and regeneration of the diaphragm were studied. In 25 rats, a subcostal incision was made and one third of the right hemidiaphragm was resected. Crosslinked porous type I collagen scaffolds (Ø ~ 14 mm) were sutured into the lesion. Rats were sacrificed at 2, 4, 8, 12 or 24 weeks after scaffold implantation. Implants were evaluated macroscopically and (immuno)histologically. Survival after surgery was 88% with no evidence of reherniation. Histological examination showed that the collagen scaffold degraded slowly and new collagen, elastin and mesothelium were deposited. Blood vessels were observed primarily at the outer borders of the scaffold; their number gradually increased in time. Muscle fibres were found on the scaffold covering up to 10% of the defect. Macroscopically, adhesion of the scaffold to the liver was observed. Use of a collagen scaffold to close a surgically created diaphragmatic defect is feasible, with evidence of new tissue formation. The use of crosslinked collagen scaffolds allows targeted modification; e.g. addition of growth factors to further stimulate growth of muscle cells.

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Section: Discussionmentioning

confidence: 98%