Fetal nucleated erythrocyte recovery: Fluorescence activated cell sorting-based positive selection using anti-gamma globin versus magnetic activated cell sorting using anti-CD45 depletion and anti-gamma globin positive selection

CD71+ erythroid cells from neonates born to women with preterm labor regulate cytokine and cellular responses

“…CD71+ erythroid cells are rarely present in the maternal circulation; yet, they seem to be a source for circulating cell-free fetal DNA [33, 34]. Therefore, we evaluated whether the direct contact between neonatal CD71+ erythroid cells and maternal PBMCs could regulate cytokine release (Figure 2A).…”

Optimization of nucleated red blood cell (NRBC) recovery from maternal blood collected using both layers of a double density gradient

“…In order to minimize contamination from mononuclear white blood cells, other authors prefer to isolate the lower interface of a double gradient (e.g. 1.107/1.077) (Larrick et al, 1979;Zheng et al, 1993;Wang et al, 2000) or the intermediate interface of a triple gradient (Gaenshirt-Ahlert et al, 1993;Kuo, 1998). The NRBC population obtained in these two cases is much more puri®ed, although some of the target NRBC would be lost at the upper layer of the gradient (Andrews et al, 1995;Hahn et al, 1999).…”

Current and emerging techniques of fetal cell separation from maternal blood

“…Furthermore only one cell parameter at a time can be measured with MACS unlike FACS where up to eight parameters can be obtained simultaneously [57]. Also, after separation, target cells still often have labeling agents bound to their surface antigens, which can sometimes cause problems for further analysis by hindering labeling with secondary antibodies [58]. Contamination of the sample can also occur if the iron oxide leaks from the magnetic beads.…”