Fetal IgG specificities against Trypanosoma cruzi antigens in infected newborns.

Abstract

A panel of Trypanosoma cruzi antigens produced by recombinant DNA techniques was used to analyze the IgM and IgG specificities present in sera from 22 mothers with chronic Chagas disease and their newborn infants. Ten of the newborns were congenitally infected and the other 12 children were healthy. While in most cases IgG specificities in the newborns mirrored those of their mothers, congenitally infected newborns had, in addition, IgG specificities that were undetectable in their mothers.' The new IgG specificities observed most frequently were against a shed acute.phase antigen

La enfermedad de Chagas congenita en la Provincia de Salta, Argentina, años 1980-1997

“…Los diferentes países del Cono sur poseen ya indicadores de infestación por triatomíneos que evidencian en el corto plazo que la vía vectorial de la transmisión por el Trypanosoma cruzi no significará un peligro real para la aparición de casos nuevos de Chagas aguda, de mantenerse y consolidarse la vigilancia entomológica emprendida 14 .…”

Tc45, a dimorphic Trypanosoma cruzi immunogen with variable chromosomal localization, is calreticulin.

“…[1][2][3][4][5][6] Although important contributions have been made to better define their potential role as immunogens or diagnostic tools, no functional characterization has been defined for the majority of the described parasite molecules. [1][2][3][4][5][6][7][8][9][10][11][12][13] The immunological functions of calreticulin in mammals have been delineated to some extent, in particular as a chaperone molecule in the process of antigen presentation to CD8ϩ T cells. Thus, when ␤ 2 -microglobulin binds to the major histocompatibility complex (MHC) class I ␣ chain, the partially folded ␣␤ 2 -microglobulin heterodimer dissociates from calnexin and binds calreticulin.…”

Serological Diagnosis of Trypanosoma rangeli Infected Patients. A Comparison of Different Methods and its Implications for the Diagnosis of Chagas' Disease

“…By contrast, the serology of T. cruzi infected patients has been widely studied and antibody responses have been demonstrated using a variety of assays and antigens, positivity being accepted when serum samples have been tested in two or three conventional assays [11]. In the last few years, recombinant DNA technology has been used to address the present difficulty in preparing purified T. cruzi parasite antigens: isolated fusion proteins and synthetic peptides modelled according to the amino acid sequences have been used as antigens in an attempt to improve the diagnosis of chronic [12][13][14][15][16][17] and acute Chagas' disease [18,19].…”