Fetal gut mesenchyme induces differentiation of cultured intestinal endodermal and crypt cells

Kédinger, Michèle; Simon‐Assmann, Patricia; Lacroix, Benoı̂t; Marxer, A.; Hauri, Hans‐Peter; Haffen, K

doi:10.1016/0012-1606(86)90183-1

Cited by 195 publications

(96 citation statements)

References 27 publications

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“…The concept that immortalized IEC cells can retain progenitor properties has been provided in xenograft experiments in the past (24). Our results support the notion that Cdx2 expression is required for IEC to initiate differentiation into mature enterocytes in the mesenchymal context.…”

Section: Discussionsupporting

confidence: 86%

“…Morphological and immunological characterization classified these cells as undifferentiated small intestinal crypt cells (42,43). The first demonstration that these cell lines could differentiate came from xenografts experiment that involved combination of IEC-17 with fetal rat gut mesoderm (24). This experimental approach confirmed that IEC-17 could generate a mature epithelium composed of the four major intestinal epithelial cell lineages (24).…”

mentioning

confidence: 96%

“…The first demonstration that these cell lines could differentiate came from xenografts experiment that involved combination of IEC-17 with fetal rat gut mesoderm (24). This experimental approach confirmed that IEC-17 could generate a mature epithelium composed of the four major intestinal epithelial cell lineages (24). The potential of these cells to differentiate in culture was provided when IEC-6 cells were engineered to conditionally express Cdx2 (54).…”

mentioning

confidence: 96%

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Hepatocyte nuclear factor-4α promotes differentiation of intestinal epithelial cells in a coculture system

Lussier

Babeu²,

Auclair³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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Normal cellular models able to efficiently recapitulate intestinal epithelial cell differentiation in culture are not yet available. The aim of this work was to establish and genetically characterize a mesenchymal-epithelial coculture system to identify transcriptional regulators involved in this process. The deposition of rat intestinal epithelial cells on human intestinal mesenchymal cells led to the formation of clustered structures that expanded shortly after seeding. These structures were composed of polarized epithelial cells with brush borders and cell junction complexes. A rat GeneChip statistical analysis performed at different time points during this process identified hepatocyte nuclear factor-4α (HNF-4α) and hepatocyte nuclear factor-1α (HNF-1α) as being induced coincidently with the apparition of polarized epithelial structures. Stable introduction of HNF-4α in undifferentiated epithelial cells alone led to the rapid induction of HNF-1α and several intestinal-specific markers and metabolism-related genes for which mRNA was identified to be upregulated during epithelial differentiation. HNF-4α was capable to transactivate the calbindin 3 gene promoter, a process that was synergistically increased in the presence of HNF-1α. When HNF-4α-expressing cells were plated on mesenchymal cells, an epithelial monolayer formed rapidly with the apparition of dome structures that are characteristics of vectorial ion transport. Forced expression of HNF-1α alone did not result in dome structures formation. In sum, this novel coculture system functionally identified for the first time HNF-4α as an important modulator of intestinal epithelial differentiation and offers an innovative opportunity to investigate molecular mechanisms involved in this process.

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Section: Discussionsupporting

confidence: 86%

mentioning

confidence: 96%

mentioning

confidence: 96%

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Hepatocyte nuclear factor-4α promotes differentiation of intestinal epithelial cells in a coculture system

Lussier

Babeu²,

Auclair³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Terminal e p i t h e l i u m differentiation may involve the specific expression of a homeo-box-containing gene and an interaction w i t h homeo-box-expressing mesenchyme. In fact, it has been s h o w n using rat chimeras that terminal differentiation of intestinal e p i t h i l i u m requires interaction w i t h gut m e s e n c h y m e (Kedinger et al 1986). …”

Section: Discussionmentioning

confidence: 99%

A mouse gene homologous to the Drosophila gene caudal is expressed in epithelial cells from the embryonic intestine.

Duprey¹,

Chowdhury²,

Dressler³

et al. 1988

Genes & Development

196

112

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“…According to their results [1,2], they may be undifferentiated epithelial crypt cells, although it has not been possible to induce their complete differentiation in vitro. Recently, Kedinger et al [3] succeeded in inducing their complete differentiation by sandwiching IEC-17 cells, which are similar to IEC-18 cells, between two homotopic fetal rat intestine mesenchymes, followed by grafting under the kidney capsule of an Correspondence address: T. Shintani. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan adult rat.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of intestinal epithelial cell line (IEC‐18) by an acid extract of rat small intestine

et al. 1989

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A factor which may induce diffe~tiation of intestinal epithelial cell lines in vitro was found in an acid extract of adult rat small intestine. The addition of a partially purified acetic acid extract of rat small intestine to IEC-18 cell culture dishes increased sucrase activity within 48 h. Nadine induration markedly decreased within 24 h. Si~~~nt development of microvilli-like structures was observed on the acid extract-treated IEC-18 cells, compared with controls. This activity of rat acid extract was heat-stable and the apparent molecular weight of the factor was 400-8UO. These findings suggested that the factor may be related to the epithelial differentiation of rat small intestinal crypt cells.

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Fetal gut mesenchyme induces differentiation of cultured intestinal endodermal and crypt cells

Cited by 195 publications

References 27 publications

Hepatocyte nuclear factor-4α promotes differentiation of intestinal epithelial cells in a coculture system

Hepatocyte nuclear factor-4α promotes differentiation of intestinal epithelial cells in a coculture system

A mouse gene homologous to the Drosophila gene caudal is expressed in epithelial cells from the embryonic intestine.

Differentiation of intestinal epithelial cell line (IEC‐18) by an acid extract of rat small intestine

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