Fetal calf serum and retinoic acid affect proliferation and terminal differentiation of a rat rhabdomyosarcoma cell line (BA-HAN-1C)

Cd, Gerharz; He, Guimei; Biesalski, H. K.; Engers, Rainer; Luley, C

doi:10.1038/bjc.1989.12

Cited by 14 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, differentiation inducers active on rhabdomyosarcoma consist of a heterogeneous group of compounds (see Introduction), with the common ability to affect growth rate. Decreased proliferation could be a condition allowing rhabdomyosarcoma cell differentiation to occur, as also suggested by the inducing effect of nutrient-deprived differentiation media (Nanni et al, 1986;Gerharz et -al., 1989a). N,N-dimethylformamide inhibited cell proliferation and increased myogenic differentiation of rhabdomyosarcoma cells, as shown by data presented here and in agreement with the results obtained with other cell lines of human and rat origin (Dexter, 1977;Nicoletti et al, 1992).…”

Section: Resultsmentioning

confidence: 99%

“…Since it has been reported that media with a low serum content can promote myogenic differentiation (Dym & Yaffe, 1979;Blau & Webster, 1981;Davis et al, 1987;Gerharz et al, 1989a;Nicoletti et al, 1992), all cultures (controls included) were switched to DMEM + 1% FCS 24 h after seeding (day 1). Dexamethasone (Sigma, St Louis, MO, USA), dissolved as 20 mM stock solution in ethanol and diluted in DMEM + 1% FCS, was added to some cultures at day I (immediately after medium replacement) at final concentrations ranging 10-1,000 nM.…”

Section: Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Uncoupling of growth inhibition and differentiation in dexamethasone-treated human rhabdomyosarcoma cells

Giovanni

Lollini²,

Dolcetti³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

Summary The effects of dexamethasone, a synthetic glucocorticoid, and of N,N-dimethylformamide on in vitro growth and differentiation and on proto-oncogene expression of human rhabdomyosarcoma cells were studied. RD/18 clone cells (derived from the embryonal rhabdomyosarcoma cell line RD) treated with 100 nM dexamethasone showed an almost complete block of differentiation: about 5% myosin-positive cells were observed after 2 weeks of culture in dexamethasone-supplemented differentiation medium, compared to 20% of untreated cultures. Dexamethasone also induced a 20-30% growth inhibition and a more flattened morphology. The treatment with N,N-dimethylformamide induced a significantly increased proportion of myosin-positive cells (reaching about 30%) and a 40% growth inhibition.Induction of differentiation inversely correlated with the levels of c-myc proto-oncogene expression: after a 2 week culture dexamethasone-treated cells showed the highest c-myc expression and N,N-dimethylformamidetreated cells the lowest. Culture conditions per se down-modulated c-erbBI and up-regulated c-jun expression, with no relationship to the differentiation pattern. Other proto-oncogenes were not expressed (c-sis, N-myc, c-mos, c-myb) or were not modulated (c-fos, c-raj).Therefore dexamethasone and N,N-dimethylformamide, both causing a decreased growth rate, showed opposing actions on myogenic differentiation and on c-myc proto-oncogene expression of human rhabdomyosarcoma cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Cellsmentioning

confidence: 99%

Uncoupling of growth inhibition and differentiation in dexamethasone-treated human rhabdomyosarcoma cells

Giovanni

Lollini²,

Dolcetti³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Nevertheless, these observations strongly suggest that the inverse linkage between proliferation and differentiation which occurs in normal embryonic rhabdomyogenesis (Nadal-Ginard, 1978) has been preserved in the rhabdomyosarcoma cell line BA-HAN-1C. This conclusion is further supported by the observation that BA-HAN-1C tumor cells can also be induced to differentiate by exposure to FCSdepeleted medium that does not support tumor-cell proliferation (Gerharz et al, 1989~).…”

Section: Discussionmentioning

confidence: 91%

Enhanced expression of the proto‐oncogenes fos and raf in the rhabdomyosarcoma cell line BA‐HAN‐1C after differentiation induction with retinoic acid and N‐methylformamide

Ramp

et al. 1990

Intl Journal of Cancer

View full text Add to dashboard Cite

BA-HAN-IC is a clonal rat rhabdomyosarcoma cell line consisting of proliferating mononuclear tumor cells, some of which spontaneously fuse to form terminally differentiated post-mitotic myotubes. Exposure of BA-HAN-IC cells to retinoic acid (RA) or N-methylformamide (NMF) resulted in a significant inhibition of proliferation (p less than 0.001) and in cellular differentiation, as evidenced by a significant increase in the creatine kinase (CK) activity (p less than 0.05) and the number of terminally differentiated post-mitotic myotubes (p less than 0.001). Furthermore, between 5% (NMF) and 30% (RA) of the mononuclear tumor cells exhibited ultrastructural features of rhabdomyogenic differentiation, not observed in their mononuclear counterparts under standard growth conditions. Although BA-HAN-IC cells responded to both inducers of differentiation, differences in time course and magnitude of both increase of differentiation and growth inhibition were observed. These effects of RA and NMF were preceded by a marked enhancement of c-raf expression which became evident 6 and 12 hr after exposure to RA and NMF, respectively, and which persisted throughout the observation period of 5 days. Furthermore, a transient expression of c-fos could be observed 15 and 30 min after exposure to RA. Our results suggest that c-raf expression might be implicated in the differentiation process of BA-HAN-IC tumor cells.

show abstract

“…However, some investigators have shown that these agents failed to induce cell differentiation in some human alveolar rhabdomyosarcoma cell lines [2,7]. These discrepancies may be due to heterogenous response among alveolar rhabdomyosarcoma cells, and this variability was observed even in a clonal rhabdomyosarcoma cell line [9,10]. We are now attempting serum-free culture and cell cloning of the NRS-1 cell line in order to elucidate the role of growth factors in human alveolar rhabdomyosarcoma.…”

Section: Discussionmentioning

confidence: 99%

In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line

Ogose

Motoyama

Watanabe

et al. 1995

Vichows Archiv A Pathol Anat

View full text Add to dashboard Cite

A newly established cell line, designated NRS-1, was derived from an alveolar rhabdomyosarcoma that developed in the left forearm of a 7-year-old girl. The cell line had a t(2; 13) chromosomal translocation. Reverse transcription-polymerase chain reaction demonstrated that 5' PAX3-3' FKHR chimeric transcript was expressed in NRS-1 cells. NRS-1 cells showed myogenic differentiation without any particular stimulus in vitro and exhibited various kinds of muscle markers. All-trans retinoic acid promoted cell differentiation in the myogenic direction. Transforming growth factor-beta (TGF-beta) inhibited myogenic differentiation of those cells and promoted cell proliferation.

show abstract

Fetal calf serum and retinoic acid affect proliferation and terminal differentiation of a rat rhabdomyosarcoma cell line (BA-HAN-1C)

Cited by 14 publications

References 30 publications

Uncoupling of growth inhibition and differentiation in dexamethasone-treated human rhabdomyosarcoma cells

Uncoupling of growth inhibition and differentiation in dexamethasone-treated human rhabdomyosarcoma cells

Enhanced expression of the proto‐oncogenes fos and raf in the rhabdomyosarcoma cell line BA‐HAN‐1C after differentiation induction with retinoic acid and N‐methylformamide

In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line

Contact Info

Product

Resources

About