Fetal bovine serum promotes the development of in vitro porcine blastocysts by activating the Rho-associated kinase signalling pathway

Guo, Shimeng; Liu, Shichao; Bou, Gerelchimeg; Guo, Jia; Jiang, Liyuan; Chai, Zhuang; Cai, Mingming; Mu, Yanshuang

doi:10.1071/rd18070

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…Numerous embryonic growth-promoting factors and other unknown cell survival components are essential during embryonic growth [ 22 ]. Adding FBS on day 4 may improve the metabolic pathways when the IVF embryos are mostly at the morula stage [ 23 ], and at that time, embryos need more nutrients and energy. It has also been reported that serum strongly inhibits the division of the first stage of the fertilized embryos and has no beneficial effect on the development from two cells to the morula stage [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

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Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo

Biswas¹,

Hyun²

2021

J Adv Vet Anim Res

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Objective: The present study aimed to explain the effect of fetal bovine serum (FBS) on the in vitro production of porcine embryos and the molecular effects of FBS on the growing of porcine embryos. Materials and Methods: Immature porcine oocytes were matured and fertilized in vitro . The resulting zygotes were cultured in porcine zygotic medium-3- until day 7 and FBS was added on day 4. Without FBS, it was treated as a control group. Quantitative real-time PCR and 2′,7′-dichloro-dihydro-fluorescein diacetate (H 2 DCFDA) molecular staining techniques were used to detect the expression patterns of apoptosis-associated genes and the accumulation of reactive oxygen species (ROS), respectively. Paired student’s t -test was used by GraphPad Prism statistical software. Results: FBS supplementation boosted blastocyst (BL) development and total cell count per BL substantially ( p < 0.05). However, hatching and hatched BLs also increased in the FBS-treated group compared to the control. We also found that ROS accumulation in FBS-treated embryos was significantly reduced ( p < 0.05) compared to the control group. The expression of the anti-apoptotic gene BCL-2 was significantly increased in FBS-treated BLs, but the pro-apoptotic gene, caspase-3 expression, was significantly reduced in FBS-treated BLs. Conclusion: Our results suggest that FBS supplementation in porcine culture media could increase porcine embryo production by decreasing ROS accumulation and increasing the anti-apoptotic gene expression in developing BLs.

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Section: Discussionmentioning

confidence: 99%

“…A growing embryo needs more energy for replicating quickly and smoothly. The presence of FBS in the culture medium influences the embryonic cell division in the BL due to increasing intracellular lipid metabolism [ 23 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo

Biswas¹,

Hyun²

2021

J Adv Vet Anim Res

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Melatonin protects against defects induced by methoxychlor in porcine oocyte maturation

Geng,

Zhang,

Shi

et al. 2024

Cell Cycle

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Trophectoderm formation: regulation of morphogenesis and gene expressions by RHO, ROCK, cell polarity, and HIPPO signaling

Alarcón

Marikawa

2022

Reproduction

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The trophectoderm (TE) is the first tissue to differentiate during the preimplantation development of placental mammals. It constitutes the outer epithelial wall of the blastocyst, and is responsible for hatching, uterine attachment, and placentation. Thus, its formation is the key initial step that enables the viviparity of mammals. Here, we first describe the general features of TE formation at the morphological and molecular levels. Prospective TE cells form an epithelial layer enclosing an expanding fluid-filled cavity by establishing the apical-basal cell polarity, intercellular junctions, microlumen, and osmotic gradient. A unique set of genes are expressed in TE that encode the transcription factors essential for the development of trophoblasts of the placenta upon implantation. TE-specific gene expressions are driven by the inhibition of HIPPO signaling, which is dependent on the prior establishment of the apical-basal polarity. We then discuss the specific roles of Ras homolog family members (RHO) and RHO-associated coiled-coil containing protein kinases (ROCK) as essential regulators of TE formation. RHO and ROCK modulate the actomyosin cytoskeleton, apical-basal polarity, intercellular junctions, and HIPPO signaling, thereby orchestrating the epithelialization and gene expressions in TE. Knowledge of the molecular mechanisms underlying TE formation is crucial for assisted reproductive technologies in human and farm animals, as it provides foundation to help improve procedures for embryo handling and selection to achieve better reproductive outcomes.

show abstract

Fetal bovine serum promotes the development of in vitro porcine blastocysts by activating the Rho-associated kinase signalling pathway

Cited by 5 publications

References 38 publications

Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo

Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo

Melatonin protects against defects induced by methoxychlor in porcine oocyte maturation

Trophectoderm formation: regulation of morphogenesis and gene expressions by RHO, ROCK, cell polarity, and HIPPO signaling

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