Fetal bovine serum alternatives

Jayme, David W.; Epstein, David; Conrad, Daragh

doi:10.1038/334547a0

Cited by 59 publications

(33 citation statements)

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“…In light of the above mentioned disadvantages and concerns, a number of methods for reducing the requirements for FBS in culture media as well as alternative animal serum substitutions were recently described (Jayme et al, 1988). The latter include the substitution by newborn or adult bovine sera, or the use of donor sera from other animal species (horse, pig, goat, etc.).…”

Section: Alternatives To Fetal Bovine Serum In Cell and Tissue Culturementioning

confidence: 99%

Serum-free cell culture: the serum-free media interactive online database

Brunner

Frank²,

Appl³

et al. 2010

ALTEX

315

232

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Prefacethe supplementation of basal culture media with animal serum of different origins is essential for cell growth, metabolism, and to stimulate proliferation ("mitogenic effect"). the sera used most widely are bovine sera of adult or newborn animals, or of fetal origin. Fetal bovine serum (FBS) is a cocktail of most of the factors required for cell attachment, growth and proliferation and is thus used as an almost universal growth supplement effective for most types of human and animal (including insect) cells. However, the use of animal serum in cell culture also bears a number of disadvantages. these disadvantages can be seen either from -a theoretical, cell biological point of view, since serum in general is an ill-defined mixture of components in culture media, -from ethical perspectives in terms of animal protection arguments regarding the harvest and collection of FBS from bovine fetuses and -in terms of recent concerns about the global supply versus demand of FBS. As a consequence, a number of strategies were developed to reduce or replace the requirement for FBS in cell culture media. -Furthermore, recent advances in biomedicine, tissue engineering and (adult mesenchymal) stem cell-based therapy demand innovative serum-alternatives of human origin for autologous cell expansion and reimplantation. Also, for the biotechno-

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Section: Alternatives To Fetal Bovine Serum In Cell and Tissue Culturementioning

confidence: 99%

Serum-free cell culture: the serum-free media interactive online database

Brunner

Frank²,

Appl³

et al. 2010

ALTEX

315

232

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“…FBS has been used for more than a century as a cell culture additive for most cell types cultured in vitro [38,39]. It has been previously shown that differences in serum composition lead to statistical differences in experimental outcomes [40].…”

Section: Discussionmentioning

confidence: 99%

“…The disadvantages of FBS, such as undefined composition or batch-to-batch variations, have been known for decades. Alternatives to FBS have been proposed and include options such as sera from other species, reduced serum media, chemically defined FBS or chemically defined serum-free media [38,39]. Using defined FBS would eliminate the necessity to test every new batch of FBS and would minimize experimental differences due to different FBS batches or types.…”

Section: Discussionmentioning

confidence: 99%

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Effect of fetal bovine serum on mineralization in silk fibroin scaffolds

Vetsch¹,

Paulsen²,

Müller³

et al. 2015

Acta Biomaterialia

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“…FBS is presently one of the most universally applicable cell culture additives for stimulation of cellular proliferation and biological production. 10 The failure of FCS to support growth of the parasite may be due to toxicity caused by high protein, high phospholipid and total cholesterol contents as previously described.…”

Section: Discussionmentioning

confidence: 99%

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Tan‐ariya¹,

Kerdmanee²,

Sarataphan³

et al. 2001

ScienceAsia

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The short-term in vitro cultivation of intraerythrocytic stages of Thai isolates of the Theileria parasite was successfully established in this study. Of the four types of culture media used in the experiment, M199 supplemented with 40% fetal bovine serum (FBS) proved to be the best medium. The mean percentage of living parasites cultured in this medium was significantly higher than that in the others, ie, M199+fetal calf serum (FCS), RPMI+FBS and RPMI+FCS (p<0.05). In addition, many developmental stages, ie, match form, ring form, and 2-piroplasm, 3-piroplasm and 4-piroplasm forms, were observed at significant numbers in M199+FBS medium after 48-72 h of cultivation. It was also demonstrated in this study that the blood samples with a high percentage of parasitemia, ie, >4.5%, should not be cultured at cell suspension concentrations higher than 12.5%, whereas those with low percentage of parasitemia, ie, <4.5%, could be cultured at any percentage of cell suspension. On the other hand, our results revealed that culturing at approximately 12.5% cell suspension provided the highest percentage of both multiplied form and living parasites. Production of an optimal cultivation system that would lead to the successful continuous in vitro cultivation of intraerythrocytic stages of Theileria parasite remains to be investigated.

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Fetal bovine serum alternatives

Abstract: With the current shortage in fetal bovine serum, researchers are turning to a variety of options for cell and tissue culture.

Cited by 59 publications

References 2 publications

Serum-free cell culture: the serum-free media interactive online database

Serum-free cell culture: the serum-free media interactive online database

Effect of fetal bovine serum on mineralization in silk fibroin scaffolds

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