Feruloyl-l-arabinose attenuates migration, invasion and production of reactive oxygen species in H1299 lung cancer cells

Fang, Hsin Yu; Wang, Hui‐Min David; Chang, Kao-Ping; Hu, Huei Ting; Hwang, Lian Je; Fu, Tzu Fun; Lin, Yin Chieh; Chang, Wei Chiao; Chiu, Tsu Pei; Wen, Zhi Hong; Fong, Y.; Chiu, Chien Chih; Chen, Bing Hung

doi:10.1016/j.fct.2013.05.019

Cited by 20 publications

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“…Fragmentation of the deprotonated ion m / z = 325 yielded daughter ions with m / z = 265, 193, and 134 ( Figure 3(a) ) typifying 5- O -feruloyl-L-arabinofuranosyl. Feruloyl arabinose was also produced through acid hydrolysis of corn bran [ 33 ]. Fragmentation of compound ( m / z = 853) resulted in daughter ions 775, 721, 643, 325, and 265 ( Figure 3(b) ) which is similar to that reported by Wang et al [ 34 ] extracted from wheat bran.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, low water solubility of FA, 0.6 mg/mL [ 45 ], may present another challenge towards bioaccessibility under physiological conditions. The solubility of FA can increase to 10 mg/mL when FA is esterified to arabinose [ 33 ]. Arabinoxylan oligosaccharides (AXOS) are getting attention for their antioxidant and prebiotic properties.…”

Section: Resultsmentioning

confidence: 99%

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Inhibition of Intestinalα-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

Malunga

Eck

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2016

Journal of Nutrition and Metabolism

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The effect of feruloylated arabinoxylan mono- and oligosaccharides (FAXmo) on mammalian α-glucosidase and glucose transporters was investigated using human Caco-2 cells, rat intestinal acetone powder, and Xenopus laevis oocytes. The isolated FAXmo from wheat aleurone and corn bran were identified to have degree of polymerization (DP) of 4 and 1, respectively, by HPLC-MS. Both FAXmo extracts were effective inhibitors of sucrase and maltase functions of the α-glucosidase. The IC50 for FAXmo extracts on Caco-2 cells and rat intestinal α-glucosidase was 1.03–1.65 mg/mL and 2.6–6.5 mg/mL, respectively. Similarly, glucose uptake in Caco-2 cells was inhibited up to 40%. The inhibitory effect of FAXmo was dependent on their ferulic acid (FA) content (R = 0.95). Sodium independent glucose transporter 2 (GLUT2) activity was completely inhibited by FAXmo in oocytes injected to express GLUT2. Our results suggest that ferulic acid and feruloylated arabinoxylan mono-/oligosaccharides have potential for use in diabetes management.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Inhibition of Intestinalα-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

Malunga

Eck

Beta

2016

Journal of Nutrition and Metabolism

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“…Free ferulic acid has low solubility (0.6 mg/mL) under physiological conditions ( Mota et al, 2008 ). However, the solubility of ferulic acid can increase to 10 mg/mL when it is esterified to arabinose ( Fang et al, 2013 ). Thus having higher concentration of feruloyl mono-/oligosaccharide may be advantageous on bio-accessibility of ferulic acid.…”

Section: Resultsmentioning

confidence: 99%

Isolation and identification of feruloylated arabinoxylan mono- and oligosaccharides from undigested and digested maize and wheat

Malunga

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2016

Heliyon

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Feruloylated arabinoxylan mono- and oligosaccharides (F-AXOS) are a subject of interest because of their prebiotic and antioxidant properties. We aimed at isolating and identifying F-AXOS from maize, wheat, wheat bran and wheat aleurone using HPLC and LC-MS/MS. Prior to extraction of F-AXOS, samples were subjected to either simulated gastric fluid with enzymes (gastric) or without enzymes (pH) or water (aqueous) at 37 °C. F-AXOS present in all samples were identified as 5-O-feruloyl-α-L- arabinofuranose and possibly 5-O-feruloyl-α-L-arabinofuranosyl-(1 → 3)-O-β-D-xylopyranose. Their mean content, measured as esterified ferulic acid (FA), was 2.5 times higher in maize (10.33 ± 2.40 μg/g) compared to wheat. Digestion under gastric or pH conditions resulted in a two-fold increase in F-AXOS in all samples. The level of F-AXOS produced during gastric or pH condition was positively correlated to the insoluble bound FA content of the sample (R2 = 0.98). 5-O-Feruloyl-α-L- arabinofuranose was the only identifiable F-AXOS released during gastric digestion. Our results suggest feruloyl arabinose is the most abundant form of F-AXOS in maize and wheat.

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“…Among these compounds are carotenoids, curcumin, and hesperidin [ 13 , 14 ]. Recently, anticancer compounds derived from plants, including goniothalamin and feruloyl- l -arabinose, were shown to inhibit the growth and metastasis of human lung cancer cells [ 15 , 16 ]. Additionally, moscatilin, isolated from the orchid Dendrobrium loddigesii , inhibited metastasis of both human breast and lung cancer cells [ 17 , 18 ].…”

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confidence: 99%

Inhibitory effect of trans-ferulic acid on proliferation and migration of human lung cancer cells accompanied with increased endogenous reactive oxygen species and β-catenin instability

Fong

Tang

et al. 2016

Chin Med

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BackgroundTrans-ferulic (FA) acid exhibits antioxidant effects in vitro. However, the underlying mechanism of trans-FA activity in cellular physiology, especially cancer physiology, remains largely unknown. This study investigated the cellular physiological effects of trans-FA on the H1299 human lung cancer cell line.MethodsThe 2,2-diphenyl-1-picrylhydrazyl assay was used to determine free radical scavenging capability. Assessment of intracellular reactive oxygen species (ROS) was evaluated using oxidized 2ʹ,7ʹ-dichlorofluorescin diacetate and dihydroethidium staining. Trypan blue exclusion, colony formation, and anchorage-independent growth assays were used to determine cellular proliferation. Annexin V staining assay was used to assess cellular apoptosis by flow cytometry. Wound healing and Boyden’s well assays were used to detect the migration and invasion of cells. Gelatin zymography was used to detect matrix metalloproteinase (MMP-2 and MMP-9) activity. Western blotting was used to detect expression levels of various signaling pathway proteins.ResultsDPPH assay results indicated that trans-FA exerted potent antioxidant effects. However, trans-FA increased intracellular ROS levels, including hydrogen peroxide and superoxide anion, in H1299 cells. Trans-FA treatment inhibited cellular proliferation and induced moderate apoptotic cell death at the highest concentration used (0.6 mM). Furthermore, trans-FA moderately inhibited the migration of H1299 cells at the concentrations of 0.3 and 0.6 mM and attenuated MMP-2 and MMP-9 activity. Trans-FA caused the phosphorylation of β-catenin, resulting in proteasomal degradation of β-catenin. Conversely, trans-FA treatment increased the expression of pro-apoptotic factor Bax and decreased the expression of pro-survival factor survivin.ConclusionVarious concentrations (0.06–0.6 mM) of trans-FA exert both anti-proliferation and anti-migration effects in the human lung cancer cell line H1299.Electronic supplementary materialThe online version of this article (doi:10.1186/s13020-016-0116-7) contains supplementary material, which is available to authorized users.

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Feruloyl-l-arabinose attenuates migration, invasion and production of reactive oxygen species in H1299 lung cancer cells

Cited by 20 publications

References 50 publications

Inhibition of Intestinalα-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

Inhibition of Intestinalα-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

Isolation and identification of feruloylated arabinoxylan mono- and oligosaccharides from undigested and digested maize and wheat

Inhibitory effect of trans-ferulic acid on proliferation and migration of human lung cancer cells accompanied with increased endogenous reactive oxygen species and β-catenin instability

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