Ferulic acid altered IL-17A/IL-17RA interaction and protected against imiquimod-induced psoriasis-like skin injury in mice

Lo, Hsin-Yi; Li, Chia-Cheng; Cheng, Hui-Man; Liu, I-Chen; Ho, Tin-Yun; Hsiang, Chien-Yun

doi:10.1016/j.fct.2019.04.060

Cited by 21 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, using an unsupervised clustering algorithm, non-lesional and as effectively treated determined specimens as well as lesional and relapse samples clustered pairwise together stating their respective similarities in molecular profiles [223]. Evaluating treatment response may not only be valuable in systemic treatments but also in radiation-based approaches like narrow band UVB irradiation (NB-UVB) [224] or topical agents like ferulic acid [225], indirubin [43], or rhodomyrtone [226]. For instance, NB-UVB irradiation was shown to exhibit systemic effects by restoring expression of the transcription factor CEBPB in PBMCs of treated patients [224].…”

Section: Prognostics and Treatmentmentioning

confidence: 99%

Review—Current Concepts in Inflammatory Skin Diseases Evolved by Transcriptome Analysis: In-Depth Analysis of Atopic Dermatitis and Psoriasis

Schwingen

Kaplan

Kurschus

2020

IJMS

View full text Add to dashboard Cite

During the last decades, high-throughput assessment of gene expression in patient tissues using microarray technology or RNA-Seq took center stage in clinical research. Insights into the diversity and frequency of transcripts in healthy and diseased conditions provide valuable information on the cellular status in the respective tissues. Growing with the technique, the bioinformatic analysis toolkit reveals biologically relevant pathways which assist in understanding basic pathophysiological mechanisms. Conventional classification systems of inflammatory skin diseases rely on descriptive assessments by pathologists. In contrast to this, molecular profiling may uncover previously unknown disease classifying features. Thereby, treatments and prognostics of patients may be improved. Furthermore, disease models in basic research in comparison to the human disease can be directly validated. The aim of this article is not only to provide the reader with information on the opportunities of these techniques, but to outline potential pitfalls and technical limitations as well. Major published findings are briefly discussed to provide a broad overview on the current findings in transcriptomics in inflammatory skin diseases.

show abstract

Section: Prognostics and Treatmentmentioning

confidence: 99%

Review—Current Concepts in Inflammatory Skin Diseases Evolved by Transcriptome Analysis: In-Depth Analysis of Atopic Dermatitis and Psoriasis

Schwingen

Kaplan

Kurschus

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…IL-17A was the other key cytokine with pleiotropic effects acting on production of chemokines, proinflammatory cytokines and nitric oxide. As recorded by literatures, IL-17A could induce keratinocytes proliferation, abnormal differentiation, as well as inflammatory cell infiltration, and alone or in combination with TNF-α increasing the expression of genes related to psoriasis in keratinocytes 42 . The fact that TNF-α antagonists (eg.…”

Section: Discussionmentioning

confidence: 63%

Green Phellodendri Chinensis Cortex-based Carbon Dots for Ameliorating Imiquimod-induced Psoriasis-like Inflammation in Mice

Zhang

Cheng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Carbon dots with multifaceted advantages have been provided hope for development brand-new nanodrug for treating thorny diseases.This study developed a green and simple calcination method to prepare novel cardon dots (CDs) as promising drug for psoriasis treatment. The as-prepared CDs using Phellodendri Chinensis Cortex (PCC) as sole precursor were characterized by a series of methods, mainly including electron microscopy, optical technology and X-ray photoelectron spectroscopy (XPS).Results: Results displayed that fluorescence (Quantum yield =5.63%) and nontoxic PCC-based CDs (PCC-CDs) with abundant chemical groups exhibited solubility and tiny sizes at average of (1.93 ± 0.53) nm, which may be beneficial for its inherent biological activity. Moreover, by using the typical imiquimod (IMQ) – induced psoriasis-like skin mouse model, we firstly demonstrated the pronounced anti-psoriasis activity of as-prepared PCC-CDs on ameliorating the appearance, psoriasis area and severity index (PASI) scores as well as histopathological morphology of both back tissues and right ears in IMQ-induced mouse. Further potential mechanisms behind the anti-psoriasis activities may be related to the anti-inflammation effects of PCC-CDs by descending the serum levels of proinflammatory cytokines (TNF-α, IL-6 and IL-17A). Conclusion: These results suggested that PCC-CDs have potential to be an anti-psoriasis candidate for clinical applications to treat psoriasis, which not only provided an evidence for further broadening the biological application of CDs, but also provided a potential hope for application nanodrugs to treat thorny diseases.

show abstract

“…The vehicle cream was also prepared without GA. Mice were randomly divided into four groups (n = 6 per group): Vaseline group (Ctl), IMQ group (IMQ), IMQ + vehicle group (IMQ + Vehicle) and IMQ + GA group (IMQ + GA). A daily topical dose of 62.5 mg of IMQ cream was used on the back skin of mouse for 14 consecutive days to induce psoriasis-like mouse model as described previously [33,34]. A dose of 50 mg/cm 2 GA cream or vehicle cream (without GA) was applied twice daily for 7 consecutive days at day 8-14 [32].…”

Section: Mice and Treatmentmentioning

confidence: 99%

“…To evaluate the potential anti-psoriatic activity of GA in vivo, IMQ was applied topically on the skin of mouse to induce psoriasis-like lesions [33,34]. Topical application of IMQ induced typical psoriatic characteristics in mice, including erythema, scaling, epidermis thickening and infiltration of immune cells, which could be improved in mice treated with GA cream, but no significant change was found in mice with vehicle cream (Fig.…”

Section: Ga Ameliorated Imq-induced Psoriasis-like Skin Lesions In Micementioning

confidence: 99%

18β-Glycyrrhetinic acid induces human HaCaT keratinocytes apoptosis through ROS-mediated PI3K-Akt signaling pathway and ameliorates IMQ-induced psoriasis-like skin lesions in mice

Gao

Guo

Nong

et al. 2020

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

Background: Psoriasis is a chronic inflammatory skin disease affecting 2-3% of the population worldwide. Hyperproliferative keratinocytes were thought to be an amplifier of inflammatory response, thereby sustaining persistence of psoriasis lesions. Agents with the ability to inhibit keratinocyte proliferation or induce apoptosis are potentially useful for psoriasis treatment. 18β-Glycyrrhetinic acid (GA), an active metabolite of glycyrrhizin, exhibits diverse pharmacological activities, including anti-inflammatory, anti-bacteria and anti-proliferation. The current study aims to evaluate the effects of GA on the proliferation and apoptosis of human HaCaT keratinocytes in vitro and investigate the effects of GA on the skin lesions of imiquimod (IMQ)-induced psoriasis-like mouse model in vivo. Methods: Cell viability was assayed by CCK-8. Flow cytometry was performed to measure apoptosis and reactive oxygen species (ROS), with Annexin V-FITC/PI detection kit and DCFH-DA probe respectively. Caspase 9/3 activities were measured using caspase activity assay kits. The protein levels of Akt and p-Akt were determined using Western blotting. IMQ was applied to induce psoriasis-like skin lesions in mice. The histological change in mouse skin lesions was detected using hematoxylin and eosin (H&E) staining. The severity of skin lesions was scored based on Psoriasis Area Severity Index (PASI). RT-PCR was employed to examine the relative expression of TNF-α, IL-22 and IL-17A in mouse skin lesions. Results: GA decreased HaCaT keratinocytes viability and induced cell apoptosis in a dose-dependent manner. In the presence of GA, intracellular ROS levels were significantly elevated. NAC, a ROS inhibitor, attenuated GAmediated HaCaT keratinocytes growth inhibition and apoptosis. In addition, GA treatment remarkably decreased p-Akt protein level, which could be restored partially when cells were co-treated with GA and NAC. LY294002 (a PI3K inhibitor) treatment significantly enhanced GA-mediated cytotoxicity. Moreover, GA ameliorated IMQ-induced psoriasis-like skin lesions in mice. Conclusions: GA inhibits proliferation and induces apoptosis in HaCaT keratinocytes through ROS-mediated inhibition of PI3K-Akt signaling pathway, and ameliorates IMQ-induced psoriasis-like skin lesions in mice.

show abstract

Ferulic acid altered IL-17A/IL-17RA interaction and protected against imiquimod-induced psoriasis-like skin injury in mice

Cited by 21 publications

References 40 publications

Review—Current Concepts in Inflammatory Skin Diseases Evolved by Transcriptome Analysis: In-Depth Analysis of Atopic Dermatitis and Psoriasis

Review—Current Concepts in Inflammatory Skin Diseases Evolved by Transcriptome Analysis: In-Depth Analysis of Atopic Dermatitis and Psoriasis

Green Phellodendri Chinensis Cortex-based Carbon Dots for Ameliorating Imiquimod-induced Psoriasis-like Inflammation in Mice

18β-Glycyrrhetinic acid induces human HaCaT keratinocytes apoptosis through ROS-mediated PI3K-Akt signaling pathway and ameliorates IMQ-induced psoriasis-like skin lesions in mice

Contact Info

Product

Resources

About