Ferulic acid alleviates sciatica by inhibiting peripheral sensitization through the RhoA/p38MAPK signalling pathway

Zhang, Di; Jing, Bei; Chen, Zhenni; Li, Xin; Shi, Huimei; Zheng, Yipeng; Chang, Shiquan; Zhao, Guoping

doi:10.1016/j.phymed.2022.154420

Cited by 19 publications

(12 citation statements)

References 33 publications

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“…Several studies have shown that the RhoA/p38MAPK pathway promotes the activation of microglia 18,34 . In this study, microglial trajectory analysis and GMI‐R1 cell experiments confirmed that the RhoA/p38MAPK pathway can lead to the M1 polarization of microglial cells.…”

Section: Discussionsupporting

confidence: 80%

“…The cells were divided into 6 groups: the control group, the LPS group (10 μg/mL LPS), the CCG group (10 μg/mL LPS + 10 μM CCG‐1423), the SB203580 group (10 μg/mL LPS + 10 μM SB203580), 18 the LPS + paeonol group (10 μg/mL LPS + 4 μM paeonol), and the paeonol group (4 μM paeonol). GMI‐R1 cells were cultured in 6‐well plates for 24 h. The medium was then discarded, and the cells were washed with PBS.…”

Section: Methodsmentioning

confidence: 99%

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Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway

Shi

Zhang

et al. 2023

CNS Neurosci Ther

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Background This study aimed to investigate the potential mechanism of paeonol in the treatment of neuropathic pain. Methods Relevant mechanisms were explored through microglial pseudotime analysis and the use of specific inhibitors in cell experiments. In animal experiments, 32 SD rats were randomly divided into the sham operation group, the chronic constrictive injury (CCI) group, the ibuprofen group, and the paeonol group. We performed behavioral testing, ELISA, PCR, Western blotting, immunohistochemistry, and immunofluorescence analysis. Results The pseudotime analysis of microglia found that RhoA, Rock1, and p38MAPK were highly expressed in activated microglia, and the expression patterns of these genes were consistent with the expression trends of the M1 markers CD32 and CD86. Paeonol decreased the levels of M1 markers (IL1β, iNOS, CD32, IL6) and increased the levels of M2 markers (IL10, CD206, ARG‐1) in LPS‐induced microglia. The expression of iNOS, IL1β, RhoA, and Rock1 was significantly increased in LPS‐treated microglia, while paeonol decreased the expression of these proteins. Thermal hyperalgesia occurred after CCI surgery, and paeonol provided relief. In addition, paeonol decreased the levels of IL1β and IL8 and increased the levels of IL4 and TGF‐β in the serum of CCI rats. Paeonol decreased expression levels of M1 markers and increased expression levels of M2 markers in the spinal cord. Paeonol decreased IBA‐1, IL1β, RhoA, RhoA‐GTP, COX2, Rock1, and p‐p38MAPK levels in the spinal dorsal horn. Conclusion Paeonol relieves neuropathic pain by modulating microglial M1 and M2 phenotypes through the RhoA/p38 MAPK pathway.

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Section: Discussionsupporting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway

Shi

Zhang

et al. 2023

CNS Neurosci Ther

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“…Thirty‐two rats were randomly divided into four groups: the sham operation group, the CCI group, the mecobalamin group, and the ferulic acid group. The rats in the sham operation group and the CCI group were given saline (0.9%, 12 ml/kg), the rats in the mecobalamin group received a gavage of mecobalamin (20 mg/kg), and the rats in the ferulic acid group received ferulic acid (100 mg/kg) by gavage 20 . The drugs were administered for 21 days.…”

Section: Methodsmentioning

confidence: 99%

Ferulic acid alleviates sciatica by inhibiting neuroinflammation and promoting nerve repair via the TLR4/NF‐κB pathway

et al. 2023

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Introduction Sciatica causes intense pain. No satisfactory therapeutic drugs exist to treat sciatica. This study aimed to probe the potential mechanism of ferulic acid in sciatica treatment. Methods Thirty‐two SD rats were randomly divided into 4 groups: sham operation, chronic constriction injury (CCI), mecobalamin, and ferulic acid. We conducted RNA sequencing, behavioral tests, ELISA, PCR, western blotting, and immunofluorescence analysis. TAK‐242 and JSH23 were administered to RSC96 and GMI‐R1 cells to explore whether ferulic acid can inhibit apoptosis and alleviate inflammation. Results RNA sequencing showed that TLR4/NF‐κB pathway is involved in the mechanism of sciatica. CCI induced cold and mechanical hyperalgesia; destroyed the sciatic nerve structure; increased IL‐1β, IL‐6, TNF‐α, IL‐8, and TGF‐β protein levels and IL‐1β, IL‐6, TNF‐α, TGF‐β, TLR4, and IBA‐1 mRNA levels; and decreased IL‐10 and INF‐γ protein levels and IL‐4 mRNA levels. Immunohistochemistry showed that IBA‐1, CD32, IL‐1β, iNOS, nNOS, COX2, and TLR4 expression was increased while S100β and Arg‐1 decreased. CCI increased TLR4, IBA‐1, IL‐1β, iNOS, Myd88, p‐NF‐κB, and p‐p38MAPK protein levels. Treatment with mecobalamin and ferulic acid reversed these trends. Lipopolysaccharide (LPS) induced RSC96 cell apoptosis by reducing Bcl‐2 and Bcl‐xl protein and mRNA levels and increasing Bax and Bad mRNA and IL‐1β, TLR4, Myd88, p‐NF‐κB, and p‐p38MAPK protein levels, while ferulic acid inhibited cell apoptosis by decreasing IL‐1β, TLR4, Myd88, p‐NF‐κB, and p‐p38MAPK levels and increasing Bcl‐2 and Bcl‐xl levels. In GMI‐R1 cells, Ferulic acid attenuated LPS‐induced M1 polarization by decreasing the M1 polarization markers IL‐1β, IL‐6, iNOS, and CD32 and increasing the M2 polarization markers CD206, IL‐4, IL‐10 and Arg‐1. After LPS treatment, IL‐1β, iNOS, TLR4, Myd88, p‐p38MAPK, and p‐NF‐κB levels were obviously increased, and Arg‐1 expression was reduced, while ferulic acid reversed these changes. Conclusion Ferulic acid can promote injured sciatic nerve repair by reducing neuronal cell apoptosis and inflammatory infiltration though the TLR4/NF‐κB pathway.

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“…Naringenin was not detected in grape, but there would be a loss of 18.79%-30.86% in other fruits and vegetables, which was more significant compared to others. Ferulic acid in cherry tomatoes was completely eliminated after HRW treatment, and as the active ingredient of many herbs, such change might pose some negative effects (Zhang et al, 2022;Li et al, 2021). The content of other phenolics like syringic acid, p-hydroxybenzoic acid, and p-coumaric acid in different fruits and vegetables showed different variation trends in content, and the exact mechanism remains unknown.…”

Section: Effects Of Hrw On Phenolic Compoundsmentioning

confidence: 99%

Effect of hydroxyl radical water treatment on functional components, volatile compounds and preservation of four different fruits and vegetables

Huang

Wang

Cheng

et al. 2022

Preprint

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Hydroxyl radical has been widely applied in the non-food fields, but whether its feasibility for treating fruits and vegetables remains unknown. In this work, effects of hydroxyl radical water (HRW) treatment on functional components, volatile compounds, and preservation properties of cherry tomato, grape, coriander and celery were investigated. Results showed that HRW treatment could be significantly effective on delaying the color change and decay of fruits and vegetables. Besides causing a slim loss of total sugars, HRW-treated samples exhibited different losses of total phenols and ascorbic acid. The total phenol and ascorbic acid of celery were lost by 17.95% and 13.01% respectively, which may be caused by excessive surface area, indicating that the applicability of HRW to celery is still to be discussed. The loss of functional components of both fruits could be controlled under 4%. Phenolic profiles and volatile compounds kept almost steadily during treatment. Notably, the increased contents of partial ester compounds might improve the sensory of fruits and vegetables, cherry tomatoes treated with HRW showed an increase of 77.28% in ethyl caproate. Moreover, the reduction of some compounds obtained from the decomposition of fatty acid hydroperoxides may enhance the fresh odor of fruits and vegetables. Accordingly, HRW treatment is a safety and environmental technology for post-harvest processing of fruits and vegetables.

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Ferulic acid alleviates sciatica by inhibiting peripheral sensitization through the RhoA/p38MAPK signalling pathway

Cited by 19 publications

References 33 publications

Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway

Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway

Ferulic acid alleviates sciatica by inhibiting neuroinflammation and promoting nerve repair via the TLR4/NF‐κB pathway

Effect of hydroxyl radical water treatment on functional components, volatile compounds and preservation of four different fruits and vegetables

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