Fertilization rather than aggregate size fractions shape the nitrite-oxidizing microbial community in a Mollisol

Han, Shun; Xiong, Xiang; Luo, Xuesong; Zeng, Luyang; Wei, Dan; Chen, Wenli; Huang, Qiaoyun

doi:10.1016/j.soilbio.2018.06.015

Cited by 26 publications

(6 citation statements)

References 20 publications

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“…The PNO was positively related to the TC, TN, SOC and TP content in the soil aggregate size fractions. This is different from that found in a Mollisol from northern China, which showed that PNO was positively correlated with C/N but not SOC content (Han, Xiong et al, ). Previous studies have indicated that some nitrite oxidizer strains may be capable of assimilating simple organic substrates, such as formate, glycerol and pyruvate, completely heterotrophically (Bock, ; Daims et al, ; Watson, Bock, Valois, Waterbury, & Schlosser, ).…”

Section: Discussioncontrasting

confidence: 90%

“…Han et al () suggested that PNO was positively linked only with the abundance of Nitrospira in soils from a rapeseed–rice rotation field trial. However, in a Mollisol of northern China, PNO of the aggregate size fractions correlated positively with Nitrobacter ‐like nxrA and negatively with Nitrospira ‐like nxrB (Han, Xiong, et al, ). In the present study, Nitrobacter abundance had a greater coefficient of correlation with PNO than that of Nitrospira , and the automatic linear modelling analysis also revealed that Nitrobacter abundance is a better predictor of PNO than Nitrospira and ammonia oxidizer abundance, which suggested that Nitribacter made a greater contribution to nitrite oxidation in this Vertisol.…”

Section: Discussionmentioning

confidence: 99%

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Soil aggregates impact nitrifying microorganisms in a vertisol under diverse fertilization regimes

Han

Luo

Tan

et al. 2019

European J Soil Science

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Ammonia oxidizers (ammonia-oxidizing archaea (AOA) and bacteria (AOB)), nitrite oxidizers (e.g., Nitrobacter and Nitrospira) and comammox Nitrospira play important roles in the nitrogen cycle in agroecosystems, yet their activities and abundance in soil microhabitats under environmental disturbances remain unclear. In this study, we collected samples from treatment plots in a field experiment established in 2010 in a lime concretion black soil in central China to investigate the effects of varying inorganic and organic fertilizer managements in a wheat-maize crop rotation, in order to investigate the impacts on the abundance and activities of ammonia and nitrite oxidizers inhabiting different microenvironments in soil, represented by three soil aggregate size fractions. The treatments were: control without fertilizer (CK), chemical fertilization (NPK), NPK and straw (NPKS), NPKS and pig manure (NPKSP) or cow manure (NPKSC). The potential nitrite oxidation activity (PNO) and soil nutrients (soil carbon, nitrogen and phosphorus content) were the highest in the microaggregates, followed by those in the macroaggregates and silt+clay fractions in all the plots. Both the ammonia and nitrite oxidizers were more abundant in the macroaggregates and microaggregates than in the silt+clay fractions. A two-way analysis of variance (ANOVA) indicated that the soil aggregate size had a significant influence on the abundance of ammonia and nitrite oxidizers and the soil nutrients. We also found a significant positive correlation between PNO and the nitrite oxidizer and AOA abundance. In addition, the abundances of the ammonia oxidizers (AOA and AOB) and nitrite oxidizers (Nitrobacter and Nitrospira)were positively correlated with each other. Overall, the soil aggregates with a higher inorganic nutrient and organic carbon content may provide a better protective microenvironment to guard against environmental disturbances and facilitate the inhabitation of ammonia and nitrite oxidizers, which cooperate in the nitrogen cycling process.

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Section: Discussioncontrasting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Soil aggregates impact nitrifying microorganisms in a vertisol under diverse fertilization regimes

Han

Luo

Tan

et al. 2019

European J Soil Science

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“…Overall, aggregate size, rather than fertilization was found to have a stronger role to play in bacterial community structures (Liao et al, 2020). However, some studies found that fertilizer addition accompanied with a sharp increase of available nutrient could more easily change bacterial community than aggregate size (Jiang et al, 2013;Jiang et al, 2014;Han et al, 2018;Lin et al, 2019). A deep understanding of the fertilization effects on soil aggregation and associated prokaryotic communities is therefore indispensable for achieving better management of soil fertility.…”

Section: G R a P H I C A L Abstractmentioning

confidence: 99%

Microscale heterogeneity of soil bacterial communities under long-term fertilizations in fluvo-aquic soils

Feng

Pan

et al. 2022

Soil Ecol. Lett.

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Differently sized soil aggregates, with non-uniform distribution of space and nutrients, provide spatially heterogeneous microenvironments for microorganisms and are important for controlling microbial community ecology and biogeochemistry in soils. Here, we investigated the prokaryotic communities within different aggregate-size fractions: macroaggregate ( > 0.25 mm), microaggregate (0.053-0.25 mm) and silt + clay ( < 0.053 mm). These were isolated from fluvo-aquic soils under 39-year fertilization strategies: no fertilizer (CK), chemical fertilizer (NPK), manure fertilizer (M), and combination of manure and chemical fertilizers (MNPK). The results showed that the proportion of macroaggregate, soil aggregate-associated organic carbon (SOC) content and aggregate stability were all significantly increased by both manure and chemical fertilizations. Organic fertilizations (M and MNPK) more effectively boosted formation and stability of macroaggregates and enhanced SOC concentration than NPK. The distribution patterns of microorganisms in aggregates were primarily shaped by fertilization and aggregate size. They explained 76.9% of the variance in bacterial community compositions. Fertilizations, especially with organic fertilizers primarily transitioned bacterial communities from slow-growing oligotrophic groups (e.g., Chloroflexi) dominance to fast-growing copiotrophic groups (e.g., Proteobacteria and Bacteroidetes) dominance across all aggregate sizes. Macroaggregates possessed a more stable bacterial community and efficiency of resource transfer, while smaller aggregates increased antagonism and weakened mutualism among bacterial communities. Overall, combination of manure and chemical fertilizers was crucial for increasing SOC content and aggregation, leading to a clear shift in bacterial community structures at aggregate scale.

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“…Our GeoChip data indicated that the taxonomic composition of the overall N‐cycling microbial community was shaped by both fertilization and soil aggregate size; moreover, aggregate size exhibited more pronounced effects than fertilization. In contrast, previous research has demonstrated that fertilization type is a major factor influencing the community structure of soil ammonia oxidizers community structure (Jiang et al ., 2014; Zhang et al ., 2017), nitrite oxidizers (Han et al ., 2018a; Han et al ., 2018b), as well as ammonifiers (Wang et al ., 2018), while particle size is a secondary factor. Additional studies are needed to further decode the roles of aggregate size and fertilization regime on the overall nitrogen cycling microbial community structure and how other factors may affect that response in different soil types.…”

Section: Discussionmentioning

confidence: 99%

“…(2010) found that the 63–250 μm sized fractions are microenvironments that promote the growth of nitrifiers and denitrifiers under different N management regimes. While soil aggregate size had no significant effects on nitrite‐oxidizer abundance and biodiversity in a red soil (Han et al ., 2018b), abundance of this group was higher in the 2000–250 and 250–53 μm soil aggregate sizes in a lime concretion black soil (Han et al ., 2018a). As mentioned above, most research on soil aggregates has focused on subcommunities within the N‐cycling functional guilds; however, some rare studies have considered the N‐cycling community in its entirety when evaluating the diversity and taxonomic composition and metabolic potential of those systems.…”

Section: Introductionmentioning

confidence: 99%

Microscale heterogeneity of the soil nitrogen cycling microbial functional structure and potential metabolism

Han

Luo

Hao

et al. 2021

Environmental Microbiology

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Summary Soil aggregates, with complex spatial and nutritional heterogeneity, are clearly important for regulating microbial community ecology and biogeochemistry in soils. However, how the taxonomic composition and functional attributes of N‐cycling‐microbes within different soil particle‐size fractions under a long‐term fertilization treatment remains largely unknown. Here, we examined the composition and metabolic potential for urease activity, nitrification, N2O production and reduction of the microbial communities attached to different sized soil particles (2000–250, 250–53 and <53 μm) using a functional gene microarray (GeoChip) and functional assays. We found that urease activity and nitrification were higher in <53 μm fractions, whereas N2O production and reduction rates were greater in 2000–250 and 250–53 μm across different fertilizer regimes. The abundance of key N‐cycling genes involved in anammox, ammonification, assimilatory and dissimilatory N reduction, denitrification, nitrification and N2‐fixation detected by GeoChip increased as soil aggregate size decreased; and the particular key genes abundance (e.g., ureC, amoA, narG, nirS/K) and their corresponding activity were uncoupled. Aggregate fraction exerted significant impacts on N‐cycling microbial taxonomic composition, which was significantly shaped by soil nutrition. Taken together, these findings indicate the important roles of soil aggregates in differentiating N‐cycling metabolic potential and taxonomic composition, and provide empirical evidence that nitrogen metabolism potential and community are uncoupled due to aggregate heterogeneity.

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Fertilization rather than aggregate size fractions shape the nitrite-oxidizing microbial community in a Mollisol

Cited by 26 publications

References 20 publications

Soil aggregates impact nitrifying microorganisms in a vertisol under diverse fertilization regimes

Soil aggregates impact nitrifying microorganisms in a vertisol under diverse fertilization regimes

Microscale heterogeneity of soil bacterial communities under long-term fertilizations in fluvo-aquic soils

Microscale heterogeneity of the soil nitrogen cycling microbial functional structure and potential metabolism

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