Fertilization in Dictyostelium: Pharmacological Analyses and the Presence of a Substrate Protein Suggest Protein Kinase C Is Essential for Gamete Fusion

Gunther, Kim E.; Ramkissoon, Shanta; Lydan, Michael A.; O’Day, Danton H.

doi:10.1006/excr.1995.1322

Cited by 10 publications

(16 citation statements)

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“…Concerning PKC, phorbol esters such as 12O-tetradecanoylphorbol-13-acetate (TPA), potent activators of PKC, have been reported to enhance the formation of zygotes in both D. discoideum and Dm 7. In contrast, staurosporine, an inhibitor of protein kinases including PKC, inhibited zygote formation in both D. discoideum and Dm 7 (Amagai, 2011;Gunther et al, 1995) and macrocyst formation in Dm7 (Kawai et al, 1993). From these results, PKC activated by the increase of intracellular Ca 2+ possibly participates in the induction of zygote formation.…”

Section: Inductive Signal Transduction Pathways For Zygote Formationmentioning

confidence: 86%

Regulatory Mechanism in Sexual and Asexual Cycles of Dictyostelium

Amagai¹

2012

Advances in Selected Plant Physiology Aspects

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Section: Inductive Signal Transduction Pathways For Zygote Formationmentioning

confidence: 86%

Regulatory Mechanism in Sexual and Asexual Cycles of Dictyostelium

Amagai¹

2012

Advances in Selected Plant Physiology Aspects

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“…13 Many, if not all, of the changes that transform fertilized mammalian egg into a zygote are mediated by a sudden surge of intracellular Ca 2' and PKC activation. 14,15 These include: cortical granule exocytosis, second polar body formation and emission, chromosome segregation, release from the meiotic metaphase II, linearization of cytoskeletal sheets and pronuclear formation.…”

Section: The Role Of Protein Kinase C In Developmentmentioning

confidence: 99%

Protein kinase C and chemical-induced abnormal palate development

Reddy

2005

Hum Exp Toxicol

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The protein kinase C (PKC) family of proteins mediates the action of growth factors and other ligands by activating a network of transcription factors that bind to TRE sequences in the promoters of many genes that regulate cell proliferation, differentiation, extracellular matrix synthesis, apoptosis and others in a cell type-, isozymeand context-specific manner. The critical role of PKC in embryonic development is indicated by early death of embryos in which one or more of these isozymes are inactivated. Our studies together with others show that palatal PKC signalling is functional and may be essential for normal palate development. Although single gene knockouts have failed to exhibit the cleft palate (CP) phenotype, owing to compensation by other kinases, many chemicals including the mycotoxin, secalonic acid D, disrupt palatal PKC signalling leading to altered palatal mesenchymal gene expression. The potential relevance of such effects to chemical-induced CP is discussed.

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“…The percentage of zygotes is elevated by the presence of increased extracellular Ca 2+ in D. discoideum and Dm-7 (Chagla et al 1980;Szabo et al 1982;Suzuki et al 1992). In this connection, phorbol esters such as 12O-tetradecanoylphorbol-13-acetate (TPA), potent activators of protein kinase C (PKC), enhance the formation of zygotes in D. discoideum (Gunther et al 1995). In contrast, staurosporine, an inhibitor of protein kinases including PKC, inhibites zygote formation in D. discoideum (Gunther et al 1995) and macrocyst formation in Dm-7 (Kawai et al 1993).…”

Section: Signal Transduction Pathways Involved In Zygote Formationmentioning

confidence: 99%

“…In this connection, phorbol esters such as 12O-tetradecanoylphorbol-13-acetate (TPA), potent activators of protein kinase C (PKC), enhance the formation of zygotes in D. discoideum (Gunther et al 1995). In contrast, staurosporine, an inhibitor of protein kinases including PKC, inhibites zygote formation in D. discoideum (Gunther et al 1995) and macrocyst formation in Dm-7 (Kawai et al 1993). Since the involvement of PKC like kinase activity in zygote formation has been mainly claimed in D. discoideum, the effect of PKC like ª 2011 The Author Development, Growth & Differentiation ª 2011 Japanese Society of Developmental Biologists kinase on zygote formation in Dm-7 was examined.…”

Section: Signal Transduction Pathways Involved In Zygote Formationmentioning

confidence: 99%

Ethylene as a potent inducer of sexual development

Amagai¹

2011

Dev Growth Differ

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A novel and critical function of ethylene, a potent plant hormone, has been well documented in Dictyostelium, because it leads cells to the sexual development (macrocyst formation) by inducing zygote formation. Zygote formation (sexual cell fusion) and the subsequent nuclear fusion are the characteristic events occurring during macrocyst formation. A novel gene, zyg1 was found to be predominantly expressed during the sexual development, and its enforced expression actually induces zygote formation. As expected, the zygote inducer, ethylene enhances the expression of zyg1. Thus the function of ethylene has been verified at all of individual (macrocyst formation), cellular (zygote formation), and molecular levels (zyg1 expression). Based on our recent studies concerning the behavior and function of the zyg1 product (ZYG1 protein), the signal transduction pathways involved in zygote formation are proposed in this review.

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Fertilization in Dictyostelium: Pharmacological Analyses and the Presence of a Substrate Protein Suggest Protein Kinase C Is Essential for Gamete Fusion

Cited by 10 publications

References 0 publications

Regulatory Mechanism in Sexual and Asexual Cycles of Dictyostelium

Regulatory Mechanism in Sexual and Asexual Cycles of Dictyostelium

Protein kinase C and chemical-induced abnormal palate development

Ethylene as a potent inducer of sexual development

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