Fertilization in Animals

Wassarman, Paul M.

doi:10.1002/(sici)1520-6408(1999)25:2<83::aid-dvg1>3.0.co;2-j

Cited by 44 publications

(15 citation statements)

References 36 publications

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“…Thus, in the spermatozoon, the acrosome constitutes a large vesicle localized at the head of the cell which liberates its content by an exocytotic event, resulting in the fusion of the acrosomal membrane with the plasma membrane of the spermatozoon head. Moreover, this acrosomal secretion is preceded by an increase in intracellular calcium, similar to that observed prior to vesicle exocytosis in neurones and neuroendocrine cells (Podell and Vacquier 1984;Wassarman 1999aWassarman , 1999bCalakos and Scheller 1996). In the oocyte, exocytosis of cortical granules located near the cell surface occurs in response to spermatozoon stimulation.…”

Section: Early Snare Expression: Gametessupporting

confidence: 56%

SNAREs during development

Hepp

Langley

2001

Cell Tissue Res

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Soluble N-ethylmaleimide-sensitive factor attached protein receptor (SNARE) molecules are implicated in many fundamental cellular processes that require membrane fusion, and the interactions of the SNARE proteins, SNAP-25, syntaxin and VAMP/synaptobrevin, have been extensively studied. This review documents recent data on their role at different stages of development. SNARE proteins are expressed very early and play important roles in fertilization and in cell division during early embryogenesis. In the developing nervous system, they are important for neurite outgrowth and transformation of the growth cone into the mature synapse. In the neuroendocrine system, in addition to neurosecretion, they are involved in processes related to morphological plasticity. Although few data exist on regulation of SNARE proteins during development, growth factors, intracellular messengers and depolarization are known to modify their cellular expression. The putative importance of these factors during development is discussed.

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Section: Early Snare Expression: Gametessupporting

confidence: 56%

SNAREs during development

Hepp

Langley

2001

Cell Tissue Res

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“…Sperm progressive motility is essential for the sperm to penetrate the zona pellucida both in vivo and in vitro, and thus this has been considered one of the most important factors in determining fertilization rates [Chiu et al 1987;Donnelly et al 1998;Turner 2006]. Fertilization is comprised of two major steps: the interaction between the sperm and the oocyte, and fusion of male and female gametes to form a pronucleus [Wassarman 1999]. Vigorous sperm motility is known to facilitate fertilization by enabling the sperm to penetrate the cumulus cell, corona radiate, and finally the zona pellucida [Liu et al 1991].…”

Section: Introductionmentioning

confidence: 99%

Sperm DNA damage or progressive motility: which one is the better predictor of fertilizationin vitro?

Lee

Lewis

2011

Systems Biology in Reproductive Medicine

104

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Sperm progressive motility has been reported to be one of the key factors influencing in vitro fertilization rates. However, recent studies have shown that sperm DNA fragmentation is a more robust predictor of assisted reproductive outcomes including reduced fertilization rates, embryo quality, and pregnancy rates. This study aimed to compare the usefulness of sperm progressive motility and DNA damage as predictive tools of in vitro fertilization rates. Here, 136 couples provided 1,767 eggs with an overall fertilization rate of 64.2%. The fertilization rate in vitro correlated with both sperm progressive motility (r 2 = 0.236; P = 0.002) and DNA fragmentation (r 2 = −0.318; P < 0.001). The relative risk of a poor fertilization rate was 9.5 times higher in sperm of men with high DNA fragmentation (>40%) compared with 2.6 times in sperm with poor motility (<40%). Further, sperm DNA fragmentation gave a higher specificity (93.3%) in predicting the fertilization rate than progressive motility (77.8%). Finally, the odds ratio to determine fertilization rate (>70%) was 4.81 (1.89-12.65) using progressive motility compared with 24.18 (5.21-154.51) using DNA fragmentation. This study shows that fertilization rates are directly dependent upon both sperm progressive motility and DNA fragmentation, but sperm DNA fragmentation is a much stronger test.

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“…Spermatozoa start to gain motility during their maturation in the epididymis [1][2][3]. When spermatozoa are in the cauda epididymis and the vas deferens, they display a sinusoidal movement pattern characterized by a symmetrical tail motion with high frequency and low amplitude [4].…”

Section: Introductionmentioning

confidence: 99%

Catsper3 and Catsper4 Are Essential for Sperm Hyperactivated Motility and Male Fertility in the Mouse1

Jin

Zheng

et al. 2007

Biology of Reproduction

160

137

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Catsper3 and Catsper4 are two recently identified testis-specific genes homologous to Catsper1 and Catsper2 that have been shown to play an essential role in sperm hyperactivated motility and male fertility in mice. Here we report that Catsper3 and Catsper4 knockout male mice are completely infertile due to a quick loss of motility and a lack of hyperactivated motility under capacitating conditions. Our data demonstrate that both CATSPER3 and CATSPER4 are required for hyperactivated sperm motility during capacitation and for male fertility. The present study also demands a revisit to the idiopathic male infertility patients who show normal sperm counts and normal initial motility for defects in sperm hyperactivated motility and for potential CATSPER gene mutations. The CATSPER channel also may be an excellent drug target for male contraceptives.

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Fertilization in Animals

Cited by 44 publications

References 36 publications

SNAREs during development

SNAREs during development

Sperm DNA damage or progressive motility: which one is the better predictor of fertilizationin vitro?

Catsper3 and Catsper4 Are Essential for Sperm Hyperactivated Motility and Male Fertility in the Mouse1

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