Fertilization, embryo development, and clinical outcome of immature oocytes from stimulated intracytoplasmic sperm injection cycles

Shu, Yaqing; Gebhardt, J.; Watt, J.; Lyon, J.; Dasig, D.; Behr, Barry

doi:10.1016/j.fertnstert.2006.08.110

Cited by 100 publications

(84 citation statements)

References 33 publications

(29 reference statements)

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“…Therefore, high concentrations of MT (10 5 nM and 10 7 nM) hampered nuclear maturation. Increased MT in follicular fluid seems to have an important role in ovulation [22], but according to the current study overdose MT maybe have detrimental effects on oocyte maturation. Previous studies indicated that physiological concentrations of ROS were necessary for meiotic resumption [25].…”

Section: Discussionmentioning

confidence: 47%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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Purpose Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM). Methods Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10 −2 , 1, 10 2 , 10 4 , 10 6 nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture. Results The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P<0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P<0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (10 6 nM). Conclusions The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects.

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Section: Discussionmentioning

confidence: 47%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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show abstract

“…It is less than half when compared to the fertilization rate of retrieved sibling MII oocytes (69.5%). A high rate of multinucleated oocytes is also found in fertilized MI oocytes injected immediately after denudation [148].…”

Section: Oocyte Maturitymentioning

confidence: 94%

“…However, embryos derived from immature oocytes do not efficiently translate into pregnancies and live births. Therefore, the clinical significance of using immature oocytes in stimulated cycles needs further investigation [148].…”

Section: Oocyte Maturitymentioning

confidence: 99%

Intracytoplasmic Sperm Injection – Factors Affecting Fertilization

Javed¹,

Michael²

2012

Enhancing Success of Assisted Reproduction

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“…This might be attributed to a poor quality oocyte caused by asynchrony in the cytoplasmic and nuclear maturation of the oocyte. In general, embryo developmental potential generated from immature oocytes in conventional IVF cycles is lower than that of sibling in vivo matured oocytes [14,15].…”

Section: Discussionmentioning

confidence: 96%

“…Actually, it is difficult to know whether embryos derived from the immature oocytes contribute to pregnancies or live births. Shu et al [14] recently reported that although immature oocytes from stimulated cycles can be normally fertilized and used to increase the number of embryos available for transfer, the embryos cannot be efficiently translated into pregnancies. This might be attributed to a poor quality oocyte caused by asynchrony in the cytoplasmic and nuclear maturation of the oocyte.…”

Section: Discussionmentioning

confidence: 99%

Live birth after in vitro maturation and vitrification of immature oocytes retrieved from conventional IVF cycle: a case report

Gunasheela

Jaykumar

et al. 2012

J Assist Reprod Genet

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Fertilization, embryo development, and clinical outcome of immature oocytes from stimulated intracytoplasmic sperm injection cycles

Cited by 100 publications

References 33 publications

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Intracytoplasmic Sperm Injection – Factors Affecting Fertilization

Live birth after in vitro maturation and vitrification of immature oocytes retrieved from conventional IVF cycle: a case report

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