Fertilization and early embryolgoy: Lineage tracing demonstrates that blastomeres of early cleavage-stage human pre-embryos contribute to both trophectoderm and inner cell mass

Mottla, Gilbert L.; Adelman, Mark R.; Hall, Jerry L.; Gindoff, Paul R.; Stillman, Robert J.; Johnson, Kurt E.

doi:10.1093/oxfordjournals.humrep.a135949

Cited by 49 publications

(26 citation statements)

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“…Statistics: two-tailed Fisher's exact test labeling one out of four blastomeres showed that within the structure of the embryo this blastomere participates in both cell types of the blastocyst, i.e. ICM and TE [43]. Some murine studies showed that, depending on the orientation of the second cleavage planes, the four blastomeres expose different developmental capabilities [32,44] and different epigenetic modifications [45,46].…”

Section: Discussionmentioning

confidence: 99%

Developmental capacity and pregnancy rate of tetrahedral- versus non-tetrahedral-shaped 4-cell stage human embryos

Cauffman

Verheyen

Tournaye

2014

J Assist Reprod Genet

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Purpose The arrangement of the blastomeres within the 4-cell stage embryo reflects the orientation of the cleavage planes during the second division. To examine their relevance, the developmental capacity and the pregnancy rate were compared between tetrahedral-shaped and non-tetrahedralshaped 4-cell stage human embryos. Methods The study included 3,546 4-cell stage embryos. The arrangement of the blastomeres at the 4-cell stage was annotated as being tetrahedral or non-tetrahedral on day 2 of preimplantation development. Embryo quality was compared on day 3 and day 5. Pregnancy rates were calculated per single embryo transfer on day 3 or day 5. Results In total, 2,803 4-cell stage embryos (79 %) displayed a tetrahedral arrangement and 743 (21 %) displayed a non-tetrahedral arrangement. Tetrahedralshaped embryos developed more into high-quality embryos on day 3 (p<0.001) and day 5 (p=0.036) and had a higher blastulation rate (p=0.009). Though, the number of high-quality embryos selected for transfer did not differ between both groups on day 3 (p=0.167) and day 5 (p~1). Three hundred thirty single embryo transfers were analysed. No significant difference in clinical pregnancy was found between both groups after transfer on day 3 (p=0.209) and day 5 (p=0.653). Conclusions The arrangement of the blastomeres according to their previous cleavage planes was correlated to the developmental potential of the 4-cell stage embryo up to the blastocyst stage. If embryo transfers are performed on day 3 and day 5 of development using embryos of adequate quality, the blastomere arrangement at the 4-cell stage had no predictable value regarding pregnancy success.

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Section: Discussionmentioning

confidence: 99%

Developmental capacity and pregnancy rate of tetrahedral- versus non-tetrahedral-shaped 4-cell stage human embryos

Cauffman

Verheyen

Tournaye

2014

J Assist Reprod Genet

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“…They proposed ideas such as cytokinesis being resumed on later cell divisions [8], that multinucleation could be a precursory step of nuclear membrane breakdown during cell division, as a temporary and reversible phenomenon [25], the presence of pseudonuclei with a possible fusion between them afterwards [8,31] and that, even though the blastomeres are totipotential, there is a preference for the chromosomally abnormal cells to allocate in the trophectoderm [34].…”

Section: Discussionmentioning

confidence: 99%

Insights on blastomere nuclearity

Gil

Gustavo

Póo

et al. 2006

J Assist Reprod Genet

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Purpose: To analyze the results of our transferred embryos, especially those that "changed" their blastomere nuclearity from Multinucleated (MN) to Mono-nucleated during development.Methods: Pregnancies where at least one MN embryo was transferred were retrospectively evaluated and categorized in order to record and follow-up on the ones that were implanted. Embryos were classified as normal (when all blastomeres were mono-nucleated on day one and two of development), corrected (multinucleated embryos on day one that became mono-nucleated on day two) and non-corrected (multinucleated either on day one, on day two or both days).Results: There were 633 transfer cycles analyzed. Thirtythree percent (206) had at least one embryo with a MN blastomere at a given stage of development. Pregnancy and implantation rates were 29.0% and 19.0% for the group of exclusively mono-nucleated embryo transfers, and 28.6% and 15.8% for the group with at least one MN embryo transferred. The pregnancy outcome for "corrected" and "noncorrected" embryos could be corroborated unequivocally in only 9 cases, with an outcome of 8 and 4 normal babies, respectively.Conclusions: Because the amount of data analyzed is not satisfactorily large, differences were not significantly different; however, a trend may exist showing that normal at term pregnancies obtained from corrected embryos are more likely to occur than those from non-corrected embryos. Nuclear observation on a daily basis should be one of the strate-Clínica de Fertilidad, Centro Médico Docente La Trinidad, Caracas, Venezuela e-mail: mgilco@cantv.net gies used to select the best embryos for transferring, to improve implantation rates and avoid multiple pregnancies.

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“…The plate was tilted at 1 degree/second (V r ) to reach a total tilt of 20 degrees. To count cells in the mouse blastocyst, the cells were stained with Hochest 33342, as previously reported (Hardy et al 1989, Mottla et al 1995. The stained blastocysts were observed by confocal microscopy (FV-1000 Olympus, Japan) and a 3D image was constructed.…”

Section: Tilting Mouse Embryo Culturementioning

confidence: 99%

Improved development of mouse and human embryos using a tilting embryo culture system

Matsuura

Hayashi

Kuroda

et al. 2010

Reproductive BioMedicine Online

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Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression, and friction force, in the fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, we developed the Tilting Embryo Culture System (TECS). The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P<0.05). Although not significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared to static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low quality embryos and those embryos cultured under suboptimal conditions (P<0.05). Here, we propose that the TECS could be a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to in the fallopian tube.

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Fertilization and early embryolgoy: Lineage tracing demonstrates that blastomeres of early cleavage-stage human pre-embryos contribute to both trophectoderm and inner cell mass

Cited by 49 publications

References 0 publications

Developmental capacity and pregnancy rate of tetrahedral- versus non-tetrahedral-shaped 4-cell stage human embryos

Developmental capacity and pregnancy rate of tetrahedral- versus non-tetrahedral-shaped 4-cell stage human embryos

Insights on blastomere nuclearity

Improved development of mouse and human embryos using a tilting embryo culture system

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