Fertilization and cleavage of bovine follicular oocytes in rabbit reproductive tracts after maturation in vitro

Fukui, Yutaka; Fukushima, Moriyuki; Ono, Hitoshi

doi:10.1002/jez.1402260116

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…A wide range of species served for the in vivo culture of bovine embryos followed by the re-collection and single transfer. In many cases the rabbit was an optimal model to firstly study the in vivo culture effect on bovine embryos (Lawson et al, 1972;Trounson et al, 1977;Fukui et al, 1983;Sirard et al, 1985;Sirard and Lambert, 1986;Sirard et al, 1988;Wall and Hawk, 1988;Ellington et al, 1990).…”

Section: Endoscopy In Cattlementioning

confidence: 99%

Animal Biotechnology III

2019

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Section: Endoscopy In Cattlementioning

confidence: 99%

Animal Biotechnology III

2019

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“…An alternative option to reduce the deleterious effects of the in vitro environment on oocytes and embryos involves using the reproductive tracts of other species. The oviduct and uterus of rabbits had been formally tested for bovine oocyte fertilization and embryo culture (FUKUI et al, 1983). In vitrofertilized bovine embryos were also cultured in vivo in the oviduct of a ewe, which increased their cryosurvival due to a reduction in lipid content (ENRIGHT et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?

Fonseca

Batista

Trevizan

et al. 2019

SCA

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We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO 2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat's uterus could present an alternative to nuclear maturation.

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Isolation, in vitro maturation, and fertilization of germinal vesicle oocytes obtained from the intact murine ovary

Randall¹,

Awadalla²,

Shivers

1990

J Assist Reprod Genet

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The purpose of this investigation was to attempt to develop a process, utilizing a murine model, which would allow more efficient harvesting from the intact ovary and maturation in vitro of germinal vesicle (GV) oocytes. The recovery process yielded 25.5 +/- 4.5 (mean +/- SE) cumulus-free GV oocytes per animal. Treatment groups included culture medium (CM) supplemented with either estradiol (E2), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), or prolactin (PRL). Among the hormone-free controls 83.2 +/- 1.6% of oocytes underwent GV breakdown, whereas 25.3 +/- 2.6% developed to the first polar body stage (PB-1) following 18 hr of incubation (n = 29 trials). Oocytes progressing to the PB-1 stage were inseminated in vitro. In vitro fertilization (IVF) of pooled in vitro matured (IVM) PB-1 oocytes (judged by two-cell formation) was 19.9%, which was significantly lower than in the group of in vivo matured oocytes (74.4%). E2 significantly increased the percentage of GV breakdown (control, 76.8 +/- 2.5%; E2 at 10 ng/ml, 92.9 +/- 2.5%, P less than 0.001; E2 at 100 ng/ml, 93.7 +/- 2.1%, P less than 0.001; and E2 at 1 micrograms/ml, 86.7 +/- 3.3%, P less than 0.05) but not PB-1 formation. Neither FSH nor hCG significantly increased GV breakdown or PB-1 formation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Fertilization and cleavage of bovine follicular oocytes in rabbit reproductive tracts after maturation in vitro

Cited by 7 publications

References 15 publications

Animal Biotechnology III

Animal Biotechnology III

Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?

Isolation, in vitro maturation, and fertilization of germinal vesicle oocytes obtained from the intact murine ovary

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