Fertility response in post-partum buffaloes following bypass nutrient supplementation

Abstract

The study was designed to deduce the effect of by-pass nutrient supplementation on follicular profile and fertility response in post-partum buffaloes. For this study, 40 post-partum buffaloes divided into 4 groups [control (10): C, no supplementation; T1 (10), bypass fat supplementation; T2 (10), bypass protein supplementation and T3 (10), bypass fat and protein supplementation] were supplemented with bypass nutrient for 90 days postpartum from day of calving. The study animals were examined at every 10 days interval for assessing the follicular profile and resumption of cyclicity. A number of large and small follicles among the groups remained comparable. However on day 90, number of large follicles was significantly reduced in T1 (0.90±0.18) and T2 (0.70±0.15) in comparison to control group (1.40±0.16) with comparable dominant follicle size among groups. Cyclicity percentage was higher in control (90%) and T2 (80%) as compared to T1 (60%) and T3 (50%), though the difference was nonsignificant. Furthermore, days to first overt estrus, number of AIs/conception and pregnancy rate remained comparable between the groups. In summary, bypass fat and protein supplementation, either alone or in combination showed no effect on follicular profile and fertility in post-partum buffaloes.

Current status of assisted reproductive technologies in buffaloes

“…Enhancing PG-based ES with either gonadotropin-releasing hormone (GnRH) or human chorionic gonadotropin (hCG) at the time of AI increases the pregnancy rate from 20% to 37% (Atabay et al, 2020). The use of T A B L E 1 Estrus synchronization protocols and results in swamp and riverine buffaloes using luteolytic and luteotropic agents or in combination with other hormones Chantaraprateep et al, 1981;Chirachaikitti et al, 1982;Chantaraprateep et al, 1983;Capitan et al, 1992;Yuan et al, 2008;Sianturi et al, 2012 PG Virakal et al, 1988 Norgestomet + PMSG 45.5 39.13 Virakal et al, 1988 Riverine PG single dose 75.0-100.0 41.5-80.0 Pant & Singh, 1991;Khattab et al, 1996;Ribeiro et al, 1998;Brito et al, 2002 PG double dose 11-12 days apart 25.0-95.0 22.8-83.0 Chohan et al, 1993;Diaz et al, 1994;Singh & Dabas, 1998;Misra et al, 2003;Srivastava, 2005. Norgestomet + E2 60.0-100 30.0-70.0 Phadnis et al, 1994;Patel et al, 2003 Norgestomet + eCG 86.0-97.6 44.6-66.7 Luthra et al, 1994;Malik et al, 2011 Norgestomet + PG 100 45.0-66.7 Utage et al, 2010;Chaudhary et al, 2015 Abbreviations: CIDR, controlled internal drug release; E2, estradiol; eCG, equine chorionic gonadotropin; GnRH, gonadotropin releasing hormone; hCG, human chorionic gonadotropin; PG, prostaglandin; PMSG, pregnant mare serum gonadotropin; PRID, progesterone releasing intravaginal device.…”