Fertility Preservation

Duncan, Francesca E.; Feinberg, Eve C.; Brannigan, Robert E.; Edmonds, Maxwell E; Ataman, Lauren; Woodruff, Teresa K.

doi:10.1016/b978-0-323-47912-7.00033-0

Cited by 2 publications

(3 citation statements)

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“…Innovative methods to restore fertility and hormone production are needed to bridge this clinical gap. An engineered tissue, such as a patient-specific organoid capable of producing gametes ex vivo, or amenable to hormone restoration when used as a transplantable bioprosthetic, could one day serve this purpose [35][36][37]. Furthermore, organoids enable the observation of testicular tubule assembly and cell-cell interactions in a higherthroughput and more accessible way than in vivo mouse models, enabling the in-depth study of normal physiology and pathological states of infertility [6,7].…”

Section: Introductionmentioning

confidence: 99%

Testicular organoid formation is a property of immature somatic cells, which self-assemble and exhibit long-term hormone-responsive endocrine function

Edmonds

Woodruff

2020

Biofabrication

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Testicular organoid models are tools to study testicular physiology, development, and spermatogenesis in vitro. However, few side-by-side comparisons of organoid generation method have been evaluated. Here, we directly tested whether the culture microenvironment is the prime determinant promoting testicular organoid self-assembly. Using Matrigel as a representative extracellular matrix (ECM), we compared multiple culture environments, 2D and 3D, ECM-free and ECM, for organoid self-assembly with immature murine testicular cells. De novo tissues were observed to self-assemble in all four culture environments tested within 72 h, however, these tissues only met requirements to be named organoids in 2D ECM and 3D ECM-free (3DF) culture methods. Based on these results, 3DF was selected for further study, and used to examine animal age as an independent variable. Organoid assembly was significantly delayed when using pubertal murine cells and entirely absent from adult murine and adult human cells. Organoid-conditioned medium and medium supplemented with 1% Matrigel did not improve organoid assembly in pubertal murine cells, but immature murine cells rescued the assembly of adult murine cells when cultured together as age-chimeric cell mixtures. In murine organoids cultured for 14 d, tubule-like structures exhibiting a highly biomimetic architecture were characterized, including some rare germ and spermatogonial stem cells. These structural organoids secreted high levels of testosterone and inhibin B over 12 weeks with preserved responsivity to gonadotropins. Collectively these studies, in which cellular self-assembly and organoid formation was achieved independent of the culture microenvironment, suggest that self-assembly is an innate property of immature testicular cells independent from, but capable of being promoted by, the culture environment. This study provides a template for studying testicular organoid self-assembly and endocrine function, and a platform for improving the engineering of functional testicular tissues.

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Section: Introductionmentioning

confidence: 99%

Testicular organoid formation is a property of immature somatic cells, which self-assemble and exhibit long-term hormone-responsive endocrine function

Edmonds

Woodruff

2020

Biofabrication

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“…Fertility preservation through OTC is a powerful technique for preserving female reproductive potential; it can preserve thousands of ovarian follicles at once and simultaneously restore endocrine function and fertility, thus allowing spontaneous conception [ 5 , 6 ] and is the only option for prepubertal girls and women who cannot delay the start of oncological treatments [ 7 ]. According to the American Society for Reproductive Medicine (ASRM), this approach is no longer considered experimental [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…According to the American Society for Reproductive Medicine (ASRM), this approach is no longer considered experimental [ 6 ]. However, there are some concerns about the graft’s survival after transplantation and the potential risk of reimplantation of tumour cells [ 5 , 6 ]. Therefore, many studies are underway to overcome these limitations and new and experimental techniques have been developed, such as in vitro maturation [ 8 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

The challenge of ovarian tissue culture: 2D versus 3D culture

et al. 2021

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Background Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. To increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells, several studies have been carried out with culture of ovarian tissue. The aim of this study was to compare a conventional (2D) culture with an alginate matrix three-dimensional (3D) model for ovarian tissue culture. Results The ovarian tissue culture within the alginate matrix (3D) was similar to 2D culture, regarding follicular density and cell apoptosis in follicles and stroma. The proliferation rate remained stable in both models for follicles, but for stromal cell proliferation it decreased only in 3D culture (p = 0.001). At 24 h of culture, cytotoxicity was lower in the 3D model (p = 0.006). As culture time increased, cytotoxicity seemed similar. Degradation of the tissue was suggested by the histological score analysis of tissue morphology after 72 h of culture. Tissue injury was greater (p = 0.01) in 3D culture due to higher interstitial oedema (p = 0.017) and tissue necrosis (p = 0.035). Conclusion According to our results, 3D culture of ovarian tissue has no advantage over 2Dculture; it is more time consuming and difficult to perform and has worse reproducibility.

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Fertility Preservation

Cited by 2 publications

References 272 publications

Testicular organoid formation is a property of immature somatic cells, which self-assemble and exhibit long-term hormone-responsive endocrine function

Testicular organoid formation is a property of immature somatic cells, which self-assemble and exhibit long-term hormone-responsive endocrine function

The challenge of ovarian tissue culture: 2D versus 3D culture

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