Fertility preservation in women undergoing cancer treatment

Rao, G. Venkata; Chian, RC; Son, WS; Gilbert, Lucy; Sl, Tan

doi:10.1016/s0140-6736(04)16320-4

Cited by 93 publications

(50 citation statements)

References 3 publications

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“…IVM is safe and costeffective especially when it is applied to women with polycystic ovary syndrome (PCOS) [3]. Occasionally, it could also be applied to poor responders and cancer patients [18].…”

Section: Introductionmentioning

confidence: 99%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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Purpose Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM). Methods Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10 −2 , 1, 10 2 , 10 4 , 10 6 nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture. Results The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P<0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P<0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (10 6 nM). Conclusions The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects.

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Section: Introductionmentioning

confidence: 99%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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show abstract

“…1). Similarly, after vitrification and warming, in vitro-matured oocytes displayed significantly greater DNA fragmentation than in vivo-matured oocytes based on the presence and length of the comet tail (98.7% vs. 53.6% and 101.4 AE 3.5 mm vs. 16 …”

Section: Dna Fragmentation Of Vitrified and Warmed Oocytesmentioning

confidence: 99%

“…Furthermore, ovarian stimulation may not be feasible for patients who must undergo gonadotoxic treatment without any delay. A novel and attractive fertility preserving strategy for these patients is retrieval of immature oocytes without gonadotropin stimulation followed by in vitro maturation (IVM) and subsequent cryopreservation of the in vitro-matured oocytes (16,17).…”

mentioning

confidence: 99%

Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification

Huang

Chen

Park

et al. 2008

Fertility and Sterility

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“…Hormone stimulation followed by oocyte aspiration, in vitro fertilization (IVF), and embryo cryopreservation is the most common approach for preserving fertility in female cancer patients prior to chemotherapy or radiation [1][2][3][4][5]. However, hormone stimulation requires a delay in cancer treatment, may be contraindicated in patients with hormone-sensitive malignancies, and is not an option for prepubertal girls.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

Fazleabas

Shikanov

et al. 2010

Biology of Reproduction

106

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Female cancer patients who seek fertility preservation but cannot undergo ovarian stimulation and embryo preservation may consider 1) retrieval of immature oocytes followed by in vitro maturation (IVM) or 2) ovarian tissue cryopreservation followed by transplantation or in vitro follicle culture. Conventional IVM is carried out during the follicular phase of menstrual cycle. There is limited evidence demonstrating that immature oocyte retrieved during the luteal phase can mature in vitro and be fertilized to produce viable embryos. While in vitro follicle culture is successful in rodents, its application in nonhuman primates has made limited progress. The objective of this study was to investigate the competence of immature luteal-phase oocytes from baboon and to determine the effect of folliclestimulating hormone (FSH) on baboon preantral follicle culture and oocyte maturation in vitro. Oocytes from small antral follicle cumulus-oocyte complexes (COCs) with multiple cumulus layers (42%) were more likely to resume meiosis and progress to metaphase II (MII) than oocytes with a single layer of cumulus cells or less (23% vs. 3%, respectively). Twenty-four percent of mature oocytes were successfully fertilized by intracytoplasmic sperm injection, and 25% of these developed to morula-stage embryos. Preantral follicles were encapsulated in fibrin-alginate-matrigel matrices and cultured to small antral stage in an FSH-independent manner. FSH negatively impacted follicle health by disrupting the integrity of oocyte and cumulus cells contact. Follicles grown in the absence of FSH produced MII oocytes with normal spindle structure. In conclusion, baboon luteal-phase COCs and oocytes from cultured preantral follicles can be matured in vitro. Oocyte meiotic competence correlated positively with the number of cumulus cell layers.This study clarifies the parameters of the follicle culture system in nonhuman primates and provides foundational data for future clinical development as a fertility preservation option for women with cancer.

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Fertility preservation in women undergoing cancer treatment

Cited by 93 publications

References 3 publications

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

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