Fertility of Frozen Rabbit Semen

Stranzinger, G.; Maurer, R. R.; Paufler, S.

doi:10.1530/jrf.0.0240111

Cited by 38 publications

(19 citation statements)

References 7 publications

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“…In this context, the basic ingredients of current sperm-freezing extenders in rabbits are a combination of permeating cryoprotectants such as acetamide or dimethyl sulphoxide (Me 2 SO) and non-permeating cryoprotective agents such as lactose, sucrose, raffinose or trehalose. In addition, egg yolk is often included as an additional protective additive in rabbit sperm-freezing extenders (Fox, 1961;Stranzinger et al, 1971;Mocé and Vicente, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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This study was conducted to elucidate the effect of different freezing extenders on two lines selected for hyperprolificacy and longevity (H and LP, respectively). In extender A, dimethyl sulphoxide (Me 2 SO) and sucrose were used as cryoprotectants. In extenders B and C, the sucrose was replaced by 20% egg yolk, and in extender C the Me 2 SO was substituted by acetamide. Semen was packaged in 0.25 ml plastic straws and cooled at 5°C for 45 min, and then was frozen in liquid nitrogen vapour for 10 min before being plunged into the liquid nitrogen. Thawing was carried out by immersing the straws in a water bath at 50°C for 10 s. Frozen-thawed semen characteristics and reproductive parameters were affected by freezing. Extender C showed significantly lower post-thawing quality traits than any of the three extenders. Acrosome integrity was significantly improved when Me 2 SO was used as cryoprotectant. Sucrose replacement by 20% egg yolk had no effect on acrosome integrity but provided significantly lower sperm motility and viability. Freezing extender affected fertility rate, total born, number of implantation sites and gestational losses, obtaining better results when extender A was used. The acrosomal integrity after frozen-thawed process showed a significant correlation with fertility at 12 th day and also at birth, indicating that an increase in acrosomal integrity leads to an increase in both fertilities (12 th day and at birth). A positive correlation between motility of semen and implantation sites was found. The post-thawing quality traits of semen were not affected by the genetic line, although LP line showed higher total born and lower foetal and gestational losses. The findings of this study suggest that freezing extender composition has a significant effect on the success of rabbit sperm for preservation, and when Me 2 SO was used as permeable cryoprotectant sucrose provided better protection compared with egg yolk and improved reproductive traits, and, on the other hand, the male genotypes used in the present study had no effect on frozen-thawed sperm parameters but negatively affected some of the reproductive parameters.

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Section: Introductionmentioning

confidence: 99%

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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“…This is because of the generally unsatisfactory results with frozen semen (Stranzinger et al, 1971;Maurer et al, 1976; Hanada and Nagase, 1980; Constantini, 1989;Fargeas, 1995). In order to better utilize semen from males of high genetic value, for production as well as for conservation and genetic programmes, use of frozen semen is important.…”

Section: Introductionmentioning

confidence: 99%

A sucrose-DMSO extender for freezing rabbit semen

Vicente

Viudes-de-Castro²

1996

Reprod. Nutr. Dev.

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Summary ― The aim of this study was to define a simple extender for freezing rabbit semen from selected males used to inseminate selected does to obtain embryos for an embryo bank. Four experiments were carried out. In the first experiment, freezing extender was defined on the basis of the results of the post-thawing motility rate. Three factors and their interactions were studied: final concentration of dimethyl sulphoxide (DMSO) (1, 1.25, 1.5 and 1.75 M), egg yolk (0% of 10 v/v) and sugar (none, or 0.5 M of glucose, lactose, sucrose, or maltose). The sucrose and 1.75 DMSO improved significantly the post-thawing motility rate (sucrose 1.75 M DMSO extender: 42 ± 3%). In the second experiment, this freezing extender was used to freeze semen from three lines. The post-thawing motility and normal acrosome rates were similar among the lines when the covariates, fresh motility and normal acrosome rates, respectively, were used in the analysis (52 ± 1 and 66 ± 1 %, respectively). In the third experiment, frozen semen from the White New Zealand line (NZ) was tested by morphological normality and viability of embryos recovered. Recovery data from NZ does inseminated with fresh and frozen semen were compared. No significant differences were found in the number of normal embryos obtained per donor doe (8.9 ± 0.5) and in their survival after vitrification (52% of live foetuses at 29 days of gestation). The sucrose-DMSO extender and freezing procedure used in this work can offer satisfactory results to apply in conservation and genetic programmes.

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“…Glycerol has been extensively used as a cryoprotectant for various cells, including mammalian spermatozoa and embryos, since Polge et al [7] reported its cryoprotective properties. In early research on freezing of rabbit spermatozoa, spermatozoa frozen with glycerol resulted in extremely low fertility [8][9][10][11]. After 1980, studies employing acetamide [12][13][14][15] or DMSO [12,[16][17][18][19][20][21][22] as a cryoprotectant have been encouraging.…”

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Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa

et al. 2006

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Abstract. The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 ± 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 ± 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 ± 3.3% and 30.2 ± 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 ± 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese white rabbit spermatozoa. Key words: Acetamide, Cryopreservation, Glycerol, Lactamide, Rabbit spermatozoa (J. Reprod. Dev. 52: [511][512][513][514][515][516] 2006) he rabbit is considered to be a valuable laboratory animal for research in teratology, immunology, microbiology, and medical and life sciences. In addition, many valuable mutants [1,2] including transgenic [3][4][5][6], have been established in the rabbit. Therefore, a need has been recognized for reliable methods to bank rabbit genetic resources efficiently in the form of cryopreserved spermatozoa. Glycerol has been extensively used as a cryoprotectant for various cells, including mammalian spermatozoa and embryos, since Polge et al. [7] reported its cryoprotective properties. In early research on freezing of rabbit spermatozoa, spermatozoa frozen with glycerol resulted in extremely low fertility [8][9][10][11]. After 1980, studies employing acetamide [12][13][14][15] or DMSO [12,[16][17][18][19][20][21][22] as a cryoprotectant have been encouraging. H o w e v e r , t h e r e i s a l i t t l e i n f o r m a t i o n o n cryoprotectants when freezing Japanese white rabbit spermatozoa.In the present study, we compared 1.0 M glycerol, lactamide, acetamide, and DMSO as cryoprotectants in egg-yolk diluent for ejaculated spermatozoa of the Japanese white rabbit. Forward progressive motility and plasma membrane

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Fertility of Frozen Rabbit Semen

Cited by 38 publications

References 7 publications

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

A sucrose-DMSO extender for freezing rabbit semen

Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa

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