Fertility of equine epididymal frozen semen using sperm motility stimulants

Melo-Ona, C. M.; Magalhães, Luiz Carlos Ona; Papa, Federico; Martin, Ian; Landim-Alvarenga, F. C.; Filho, José Nicolau Próspero Puoli; Soares, R. F.; Alvarenga, Marco Antônio; Monteiro, Gabriel Augusto; Dell’Aqua, J.A.

doi:10.1016/j.jevs.2012.06.062

Cited by 1 publication

(3 citation statements)

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“…Caffeine is another substance used for the purpose of increasing sperm motility indicators in different animal species (Fayed 1996;Barakat et al 2015) but its effect on ejaculated or epididymal stallion spermatozoa was not investigated very deeply and the results differed (Melo-Oña et al 2012;Stephens et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…That corresponds with the studies done on ejaculated spermatozoa (Stephens et al 2013), nevertheless, the exact mechanism has not been explained yet. Melo-Oña et al (2012) reported positive results on equine ejaculated and epididymal spermatozoa that were reached when CAF was mixed with other substances (medium containing heparin, CAF, and bovine serum albumin). Fertility was not affected, so we can conclude that CAF has no deleterious effect on fertility.…”

Section: Discussionmentioning

confidence: 99%

“…Pentoxifylline (PTX) and caffeine (CAF) are readily available and their effect on ejaculated sperm motility has been studied before (Gradil and Ball 2000;Melo-Oña et al 2012;Stephens et al 2013;Tsunoda et al 2015). However, information about their effect on epididymal spermatozoa is scarce (Guasti et al 2013).…”

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confidence: 99%

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Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

et al. 2021

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Two substances at two concentrations each were added to frozen-thawed stallion epididymal spermatozoa to improve motility indicators, which were assessed 30, 60, 120, 180 and 240 min after thawing. Pentoxifylline was added at concentrations of 3.6 mM (1 mg/ml) and 7.18 mM (2 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa throughout the study. The other substance, caffeine, was added at concentrations of 2 mM (0.4 mg/ml) and 5.5 mM (1 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa only 30 and 60 min after thawing. Subsequently, 180 min after thawing, improvement was found only in total sperm motility.

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