Fertility of doubled monoploids ofSolanum phureja

M'Ribu, H. Kaburu; Veilleux, Richard E.

doi:10.1007/bf02852295

Cited by 11 publications

(6 citation statements)

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“…Upon development of dihaploids they pass to homozygous state, which is manifested in a decrease in fertility and appearance of sterile forms. Estimates of male fertility in doubled monoploid are in favor of hypothesizing inbreeding as the main reason of male sterility in potato dihaploids [25]. Hybridization of dihaploids with primitive cultivated and wild diploid potato species, which eliminates the inbreeding depression effect, contributes to recovery of their fertility [8].…”

Section: E V E L O P M E N T O F P O T a T O D I H A P L O I D Smentioning

confidence: 99%

DEVELOPMENT OF INITIAL MATERIAL FOR MARKER ASSISTED POTATO (Solanum tuberosum L.) PARENTAL LINE BREEDING AT THE DIPLOID LEVEL (review)

Yermishin¹,

Воронкова²

2017

S-h. biol.

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A b s t r a c tIt is important for improving the efficacy of potato breeding to have parental lines that are multiplex for many genes of agronomic characters, first of all genes of resistance to pests and diseases (that have two or more dominant alleles in the locus) (J.E. Bradshaw et al., 1994). Parental lines that are multiplex for several resistance genes occur not often (A.P. Yermishin et al., 2016) and, owing to biological peculiarities of potato crop, they can be produced only by means of breeding at the diploid level. In the presented review the genetic principles are described and an experience of their use by authors in research devoted to the development of the initial material applicable for effective marker assisted potato parental line breeding at the diploid level. The material includes the collection of initial dihaploids originated from potato varieties which were selected for viability, tuber performance, cultivar characters and DNA-markers of pest and diseases resistance genes; the diploid breeding material having wide range of late blight and virus resistance genes, introgressed from wild potato species by means of original methods of overcoming interspecific reproductive barriers; the diploid lines -donors of self-incompatibility gene and high male fertility genes that are effective as in heterozygous as well as in homozygous condition; the diploid lines -donors of genes of male fertile FDR (first division restitution) 2n-gamete formation. The best diploid lines having a complex of pest and diseases resistance genes, selected as the result of diploid breeding (on the basis of initial dihaploids, interspecific hybrids and donors of fertility) were used for production of multiplex tetraploid parental lines by means of mitotic chromosome doubling. Diploid parental lines suitable for hybridization with potato varieties (for meiotic polyploidization) were bred using lines-donors of genes of male fertile FDR 2n-gamete formation. The diploid initial material developed as the result of the research is of the interest for prospective alternative directions of potato breeding using selection at the diploid level: breeding diploid potato varieties as well as parental lines for production of hybrids and hybrid populations for true potato seed technology.

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Section: E V E L O P M E N T O F P O T a T O D I H A P L O I D Smentioning

confidence: 99%

DEVELOPMENT OF INITIAL MATERIAL FOR MARKER ASSISTED POTATO (Solanum tuberosum L.) PARENTAL LINE BREEDING AT THE DIPLOID LEVEL (review)

Yermishin¹,

Воронкова²

2017

S-h. biol.

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“…We have doubled the chromosome number of anther-derived monoploids of S. phureja to generate homozygous lines (M'Ribu and Veilleux, 1992). These doubled monoploids have exhibited female fertility and could be crossed with heterozygous diploid potato selections.…”

Section: Simple Sequence Repeat (Ssr) and Rapd Analysismentioning

confidence: 99%

Anther Culture of Potato and Molecular Analysis of Anther-derived Plants as Laboratory Exercises for Plant Breeding Courses

Veilleux¹

1999

horttech

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Anther culture has been one of the most successful techniques for generating haploid plants over a wide range of species. It is a reasonably simple procedure that can be accomplished successfully without sophisticated laboratory facilities; yet, the plants generated through anther culture can be used to demonstrate the application of many modern methods that have direct applicability to plant breeding. Anthers of diploid potato clones that have been selected for competence in anther culture can be cultured in a simple medium to yield androgenic embryos after 5 weeks. Plant regeneration requires an additional 3 to 4 weeks. Regenerated plants should be large enough 2 weeks after transfer to basal medium for ploidy determination by any of three methods depending on available facilities: chromosome counts in root tips; chloroplast counts in stomatal guard cells; or flow cytometry of nuclei released from in vitro plantlets. DNA can be extracted from anther-derived plantlets using a rapid extraction procedure to demonstrate segregation of PCR (polymerase chain reaction)-based markers such as RAPD (randomly amplified polymorphic DNA), RAMPs (randomly amplified microsatellite polymorphisms), or microsatellites. Microsatellite markers that were heterozygous in the anther donor can be used to verify haploidy in anther-derived plants. If an anther culture laboratory is scheduled early in a semester, such molecular analysis can be planned for late in the same semester.

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“…A modest effort was made to enlarge the germplasm base by screening for other anther culture-competent selections within the adapted Phureja seedling population to generate a more genetically diverse homozygous Phureja germplasm base (Johnson et al 2001). Over the years, various efforts were made to improve the vigor of Phureja monoploids through somatic hybridization (Haynes 1972;Johnson et al 2001;Lightbourn and Veilleux 2007) and outcrossing followed by re-extraction of monoploids (M'Ribu and Veilleux 1992;Paz and Veilleux 1997). The monoploids and doubled monoploids were maintained in vitro for many years at Virginia Tech and some were deposited in the Potato Gene Bank (http://www.ars-grin.…”

Section: Introductionmentioning

confidence: 99%