Fertility in Holstein × Gyr cows in a subtropical environment after insemination with Gyr sex-sorted semen

Mellado, M.; Coronel, Francisco; Estrada, Alejandra Rocha; Rincón, F.

doi:10.1007/s11250-010-9585-3

Cited by 14 publications

(21 citation statements)

References 22 publications

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“…Em estudo realizado anteriormente em nosso laboratório (CARVALHO et al, 2010) SARTORI et al, 2004;BODMER et al, 2005;ANDERSSON et al, 2006;PEIPPO et al, 2009;DERJANETTE et al, 2010;MELLADO et al, 2010;UNDERWOOD et al, 2010aUNDERWOOD et al, , 2010bSALES et al, 2011; (BLONDIN et al, 2009;CARVALHO et al, 2010). Estes autores sugerem que os danos nos espermatozoides podem ser causados pela exposição ao corante…”

Section: Discussionunclassified

“…Entretanto, quando o sêmen sexado é utilizado na inseminação artificial (IA) e transferência de embriões (TE) as taxas de gestação ou produção de embriões são inferiores quando comparadas às obtidas com sêmen não sexado (SEIDEL et al, 1999;SARTORI et al, 2004;BODMER et al, 2005;ANDERSSON et al, 2006;PEIPPO et al, 2009;DERJANETTE et al, 2010;MELLADO et al, 2010;UNDERWOOD et al, 2010aUNDERWOOD et al, , 2010bSALES et al, 2011;.…”

Section: Introductionunclassified

“…Treated DNA was used for PCR amplification, which was cloned, transformed into E. coli and sequenced for analyses of the methylation patterns. (SEIDEL et al, 1999;SARTORI et al, 2004;BODMER et al, 2005;ANDERSSON et al, 2006;PEIPPO et al, 2009;DERJANETTE et al, 2010;MELLADO et al, 2010;UNDERWOOD et al, 2010aUNDERWOOD et al, , 2010bSALES et al, 2011;. Da mesma forma, alguns autores também têm observado baixas taxas de blastocisto produzidas in vitro quando sêmen sexado é utilizado (MORTON et al, 2007;BERMEJO-ALVAREZ et al, 2008 (CURCHOE et al, 2005;PERECIN et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…In that model, the features with greatest relevance to NS, SX, SY and SXY were shape descriptors, such as roughness of membrane (1.39±0.01 1.37±0.02, 1.37±0.02 and 1.37±0.02), circularity ratio ( (GARNER et al, 1993). Entretanto, as menores taxas de produção de embriões em programas de transferência de embriões (TE) ou taxa de gestação na inseminação artificial (IA) (SEIDEL et al, 1999;SARTORI et al, 2004;BODMER et al, 2005;ANDERSSON et al, 2006;PEIPPO et al, 2009;DERJANETTE et al, 2010;MELLADO et al, 2010;UNDERWOOD et al, 2010aUNDERWOOD et al, , 2010bSALES et al, 2011; VAN MUNSTER et al, 1999). Desta forma, possíveis erros e limitações das técnicas, poderiam ser responsáveis pelos resultados inconstantes.…”

Section: Introductionunclassified

“…De fato, em um estudo realizado em nosso laboratório (CARVALHO et al, 2010), foi identificado que apesar de o processo de sexagem afetar algumas características estruturais da célula espermática, isto não comprometeu sua capacidade de fecundar e produzir embriões in vitro. Entretanto, alto custo e reduzida fertilidade em programas de inseminação artificial (IA) ou transferência de embriões (SEIDEL et al, 1999;SARTORI et al, 2004;BODMER et al, 2005;ANDERSSON et al, 2006;PEIPPO et al, 2009;DERJANETTE et al, 2010;MELLADO et al, 2010;UNDERWOOD et al, 2010aUNDERWOOD et al, , 2010bSALES et al, 2011;, continuam sendo um problema no uso do sêmen sexado in vivo. Uma possibilidade para explicar esse problema é de que o processo de sexagem causaria um dano espermático que não afeta a taxa de fecundação ou produção de blastocisto in vitro, mas que poderia influenciar a taxa de concepção e gestação quando utilizado in vivo.…”

Section: Introductionunclassified

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Aspectos moleculares, estruturais e funcionais de espermatozoides bovinos sexados por citometria de fluxo

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À minha família, especialmente aos meus pais, Joaquim e Ana, que nos momentos de minha ausência dedicados ao estudo, sempre fizeram entender que o futuro é feito da dedicação constante no presente. Dedico a vocês minha conquista, com a mais profunda admiração, respeito e carinho. AGRADECIMENTOSAo meu pai por despertar em mim esta paixão. A minha mãe, que sempre me guiou serena e sabiamente. As minhas três irmãs que sempre me incentivaram e acreditaram.Ao brilho nos olhos do meu avô que se alegra e me motiva em cada visita.Aos meus padrinhos, que há muito são considerados como pais.Ao meu sobrinho Joaquim, que ainda inconsciente da importância, me alegra e descontrai com suas brincadeiras.À co-orientadora, Margot Alves Nunes Dode, por tanto se dedicar e acreditar em mim com seus ensinamentos, orientação, paciência e estímulo.Ao orientador Roberto Sartori Filho, pela oportunidade concedida, me acolhendo sinceramente desde o princípio e orientando nos momentos necessários.Aos pesquisadores Maurício Machaim Franco, pela orientação em parte do trabalho e paciência em minhas muitas perguntas e Eduardo de Oliveira Mello, pela ajuda e discussões dentro do laboratório.

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Section: Discussionunclassified

Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

Section: Introductionunclassified

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Sexing sperm of domestic animals

Espinosa-Cervantes

Córdova-Izquierdo

2012

Trop Anim Health Prod

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The ability to preselect or predetermine the sex of offspring prior to conception is a highly desired technological tool for assisted female breeding programs specifically for milk production, and in males, for meat production and increasing livestock numbers. The current technology is based on the well-known differences in X- and Y-sperm in the amount of DNA. The technology uses modified flow cytometric instrumentation for sorting X- and Y-bearing sperm. The method can be validated on the basis of live births, laboratory reanalysis of sorted sperm for DNA content, and embryo biopsy for sex determination. Currently, the sex of animals has been predetermined with 90 % accuracy by sexing spermatozoa. In the bovine breeding industry, flow cytometric sperm sexing has not fulfilled its original promise. Sexed sperm doses are too expensive for widespread application while the fertility of sexed sperm doses is lower than unsexed ones. Essentially all bovine sexed semen is frozen and then applied through artificial insemination (AI) or in vitro fertilization. There is still a need in the animal breeding industry to develop a technique for sperm sexing that provides sufficient spermatozoa for AI doses, does not compromise sperm fertility, and is widely applicable to a range of species. In this review, we will summarize the current state-of-the-art in sex preselection in domestic animals and some wildlife species using flow cytometric sperm-sorting of X from Y sperm based on DNA differences.

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Effects of month of breeding on reproductive efficiency of Holstein cows and heifers inseminated with sex-sorted or conventional semen in a hot environment

Mellado

Sepúlveda

Cruz

et al. 2013

Trop Anim Health Prod

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The main objective of this study was to assess the effect of month of breeding on reproduction performance of Holstein heifers and cows inseminated with sex-sorted or conventional semen in a hot environment. Pregnancy per artificial insemination (P/AI; 64,666 services over an 8-year period) both in heifers (n = 22,313) and cows (n = 42,353) from a large dairy herd in northern Mexico (26°N) were evaluated with the GENMOD procedure of SAS, with respect to month of AI. Overall, P/AI with sex-sorted semen was greater (P < 0.01) in heifers (41.6 %) than cows (17.3 %). P/AI for cows serviced with conventional semen was 10 % points higher (P < 0.01) in January and December (31 vs. 21 %) than cows serviced with sex-sorted semen. While there was no difference in P/AI between the sex-sorted sperm and conventional semen in cows inseminated in July (16 and 18 %, respectively), P/AI plummeted for both groups of cows during the summer and fall (more severe heat stress). P/AI was not different between heifers serviced with sex-sorted or conventional semen during the hottest months of the year (July to October). However, during the coldest month of the year (January and February), P/AI was 10 percentage points greater (P < 0.01) in heifers serviced with conventional than sex-sorted semen. It was concluded that in this hot climate cow and heifer fertility declined in the summer and fall when inseminated with conventional semen. However, the use of sex-sorted semen during summer and fall did not compromise the breeding success in heifers. Thus, this data suggest that sex-sorted semen promotes some embryonic thermoprotective mechanism, which leads to a marginal summer and fall fertility depression with this type of semen in this particular hot environment.

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Fertility in Holstein × Gyr cows in a subtropical environment after insemination with Gyr sex-sorted semen

Cited by 14 publications

References 22 publications

Aspectos moleculares, estruturais e funcionais de espermatozoides bovinos sexados por citometria de fluxo

Aspectos moleculares, estruturais e funcionais de espermatozoides bovinos sexados por citometria de fluxo

Sexing sperm of domestic animals

Effects of month of breeding on reproductive efficiency of Holstein cows and heifers inseminated with sex-sorted or conventional semen in a hot environment

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