Fertility control: the role of magnesium transporters in pollen development

Mueller‐Roeber, Bernd; Arvidsson, Samuel

doi:10.1038/cr.2009.82

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Ionome profiling of AhZIP6‐1 indehiscent anthers also revealed a magnesium accumulation (Figure 5a). Proper magnesium homeostasis has been linked to plant reproduction, especially to pollen development (Chen et al, 2009; Dhaka et al, 2020; Li et al, 2008; Mueller‐Roeber & Arvidsson, 2009; Xu et al, 2015). For instance, the magnesium transporter MGT5 is important for tapetum function (Xu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

ZRT‐IRT‐Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility

Spielmann

Detry

Thiébaut

et al. 2021

Plant Cell & Environment

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Metallic micronutrients are essential throughout the plant life cycle. Maintaining metal homeostasis in plant tissues requires a highly complex and finely tuned network controlling metal uptake, transport, distribution and storage. Zinc and cadmium hyperaccumulation, such as observed in the model plant Arabidopsis halleri, represents an extreme evolution of this network. Here, non‐ectopic overexpression of the A. halleri ZIP6 (AhZIP6) gene, encoding a zinc and cadmium influx transporter, in Arabidopsis thaliana enabled examining the importance of zinc for flower development and reproduction. We show that AhZIP6 expression in flowers leads to male sterility resulting from anther indehiscence in a dose‐dependent manner. The sterility phenotype is associated to delayed tapetum degradation and endothecium collapse, as well as increased magnesium and potassium accumulation and higher expression of the MHX gene in stamens. It is rescued by the co‐expression of the zinc efflux transporter AhHMA4, linking the sterility phenotype to zinc homeostasis. Altogether, our results confirm that AhZIP6 is able to transport zinc in planta and highlight the importance of fine‐tuning zinc homeostasis in reproductive organs. The study illustrates how the characterization of metal hyperaccumulation mechanisms can reveal key nodes and processes in the metal homeostasis network.

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Section: Discussionmentioning

confidence: 99%

ZRT‐IRT‐Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility

Spielmann

Detry

Thiébaut

et al. 2021

Plant Cell & Environment

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“…So far it was shown that disruption of specific Mg cations transporters in Arabidopsis (Atmgt5, Atmgt9 and Atmgt4) led to pollen abortion (Li et al, 2008; Chen et al, 2009; Li et al, 2015) suggesting an important role of Mg 2+ during pollen development (Mueller-Roeber and Arvidsson, 2009). In our study application of Mg 2+ did not affect the pollen grains isolated from flower buds whereas in the mature pollen grains led not only to PM depolarization but also induced shrinkage of the PM surface area as a result of water loss (Fig.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis annexin 5 controls plasma membrane properties in mature pollen grains

Lichocka

Krzymowska

Górecka

et al. 2021

Preprint

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In Arabidopsis, a dry stigma surface enables a gradual hydration of pollen grains by a controlled release of water. Occasionally the grains may be exposed to extreme precipitations that cause rapid water influx, swelling and eventually lead to pollen membrane (PM) rupture. In metazoans, calcium- and phospholipid-binding proteins, referred to as annexins participate in repair of the plasma membrane damages. It remains unclear, however, how this process is conducted in plants. Here, we examined whether the plant annexin 5 (ANN5), the most abundant member of the annexin family in pollen, is involved in the restoration of PM integrity. We analyzed a cellular dynamics of ANN5 in the pollen grains undergoing in vitro and in vivo hydration. We observed a transient ANN5 association to PM during the in vitro hydration that did not occur in the pollen grains being hydrated on the stigma. To simulate a rainfall, we performed spraying of the pollinated stigma with deionized water that induced ANN5 accumulation at PM. Similarly, calcium or magnesium application affected PM properties and induced ANN5 recruitment to PM. Our data suggest a model, in which ANN5 is involved in the maintenance of membrane integrity in pollen grains exposed to osmotic or ionic imbalances.

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Soil metals, chloroplasts, and secure crop production: a review

Solymosi

Bertrand²

2011

Agron. Sustain. Dev.

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with unbalanced metal concentrations are used worldwide for crop cultivation. Even though plants are able to develop strategies to cope with metal stress, either metal deficiency or excess, this unbalance affects the whole plant. Chloroplasts are key organelles for organic matter synthesis and biomass production. Under metal stress, chloroplasts suffer severe alterations of their ultrastructure, associated with profound molecular and metabolic damages. These alterations are accompanied by unbalanced metal distribution in plants, in particular in edible crop organs. Toxic metals get either accumulated or there is a deficiency of nutrients, resulting in a weak nutritional value. Nonetheless, there is more and more knowledge on the functioning and regulation of metal transporters in plants. Such knowledge will allow growing crops with well-balanced metal concentrations in edible parts even on metal unbalanced soils. This review shows that almost all vital functions of chloroplasts, such as photosynthesis, CO 2 fixation, nitrogen and sulfur assimilation, and protein and nucleic acid metabolism, require metals. Therefore, the uptake of essential metals is necessary for the proper functioning of chloroplasts and, in turn, for crop productivity. We describe nutrient uptake mechanisms of plants and processes that influence essential and non-essential metal concentrations in different plant organs. We present an overview of metal transporters in chloroplasts. Several questions still need to be elucidated about the uptake and the trafficking of essential and non-essential metals into and within chloroplasts. Similarly to transporters present in other cellular compartments, the carriers are often not metal-specific. Therefore, essential and non-essential metals may compete for carriers. As a result, unbalanced soil metal concentrations can be reflected in the plants and in the chloroplasts. Metal deficiency or excess causes reduced growth and decreased productivity of crops. It can lead to human malnutrition. Several complex physiological processes can be responsible for the reduced biomass observed in plants with unbalanced metal concentration. In this review, we have focused on the structural and functional alterations of chloroplasts under metal deficiency or excess. Interestingly, besides specific differences, our data indicate several similarities in the response of chloroplasts to metal deficiency or excess. Indeed, oxidative stress and several ultrastructural alterations, e.g., increase in the number and size of plastoglobuli, disorganized grana and disturbed thylakoids, and swelling of the intrathylakoidal space, are observed in both cases. This indicates that changes in chloroplast ion homeostasis rather than the specific effect of a metal are responsible for decreased plant productivity. Therefore, sustainable agriculture has to take into consideration solutions that enable undisturbed metal and ion homeostasis in chloroplasts of crop plants grown even in soils with unbalanced metal concentrations.Abstract An incr...

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Fertility control: the role of magnesium transporters in pollen development

Cited by 4 publications

References 10 publications

ZRT‐IRT‐Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility

ZRT‐IRT‐Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility

Arabidopsis annexin 5 controls plasma membrane properties in mature pollen grains

Soil metals, chloroplasts, and secure crop production: a review

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