Fertility-associated proteins in Nelore bull sperm membranes

Roncoletta, Marcelo; Morani, Erika da Silva Carvalho; Esper, César Roberto; Barnabé, Valquíria Hyppólito; Franceschini, P. H.

doi:10.1016/j.anireprosci.2005.03.014

Cited by 46 publications

(44 citation statements)

References 27 publications

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“…BSP3 has previously been found to be more abundant on sperm membrane from subfertile Nellore bulls (Roncoletta et al 2006). Similarly, BSP5 was found in higher concentration in accessory sex gland fluid from subfertile Holstein bulls (Moura et al 2006b).…”

Section: Proteomic Comparison Of Sperm Proteins From Bullsmentioning

confidence: 83%

Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes

D’Amours

Frenette²,

Fortier³

et al. 2010

Reproduction

145

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Intrinsic factors such as proteins modulate the fertilising ability of male gametes. We compared detergent-extracted sperm protein composition of bulls with different fertility indexes in order to highlight putative fertility markers of sperm. Frozen semen from 23 Holstein bulls with documented fertility was used. According to their 'fertility solution' (SOL), as calculated by the Canadian dairy network, bulls were divided into four groups: high fertility (HF) (SOLO3.0; nZ6), medium-HF (2.9OSOLO2.0; nZ5), medium-low fertility (K2.8OSOLOK4.9; nZ8) and low fertility (LF; SOL!K5.0; nZ4), with a SOLZ0 being the average. Triton X-100 protein extracts from ejaculated spermatozoa were subjected to two-dimensional difference gel electrophoresis, and polypeptide maps were quantitatively analysed by ImageMaster software. Nine protein spots showed significant differences between the HF and LF groups, and eight of these proteins were identified by liquid chromatography-tandem mass spectrometry. T-complex protein 1 subunits 3 and q (CCT5 and CCT8), two isoforms of epididymal sperm-binding protein E12 (ELSPBP1), proteasome subunit a type-6 and binder of sperm 1 (BSP1) were more expressed in the LF group than in the HF group. On the other hand, adenylate kinase isoenzyme 1 (AK1) and phosphatidylethanolamine-binding protein 1 (PEBP1) were more expressed in the HF group than in the LF group. The presence and expression level of ELSPBP1, BSP1, AK1 and PEBP1 were confirmed by western blot. A linear regression model established that CCT5 and AK1 explained 64% (P!0.001) of the fertility scores. The reported functions of these proteins are in agreement with a putative involvement in defective sperm physiology, where lower or higher levels can jeopardise sperm ability to reach and fertilise the oocyte.

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Section: Proteomic Comparison Of Sperm Proteins From Bullsmentioning

confidence: 83%

Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes

D’Amours

Frenette²,

Fortier³

et al. 2010

Reproduction

145

View full text Add to dashboard Cite

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“…Lessard et al (2011) found that the BSP1 levels on sperm from an infertile bull were higher than those of his sire. Roncoletta et al (2006) reported that the levels of BSP3 were higher in lower fertility Nelore bulls. The field studies provide strong indication that the level of BSP protein coating on sperm plays a crucial role for fertilization in vivo.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Ardón

Suárez

2013

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Artificial insemination with frozen semen allows affordable, worldwide dissemination of gametes with superior genetics. Nevertheless, sperm are damaged by the cryopreservation process. Elucidating the molecular effects of cryopreservation on sperm could suggest methods for improving fertility of frozen/thawed semen. This study was undertaken to examine the effect of cryopreservation on the coating of sperm by binder of sperm (BSP) proteins in seminal plasma. BSP proteins are secreted by the seminal vesicles and coat the surface of sperm by partially intercalating into the outer leaflet of the sperm plasma membrane. The BSP proteins are known to play roles in the formation of the oviductal sperm storage reservoir and in sperm capacitation. We investigated the effects of cryopreservation on the sperm BSP protein coat using Bovipure to separate live sperm from extended semen and then assaying the amounts of BSP proteins on sperm using quantitative western blotting with custom-made antibodies against unique sequences of each BSP protein. Greater amounts of all three BSP proteins (BSP1, BSP3, and BSP5) were detected on frozen/thawed sperm than on fresh sperm. Furthermore, the reduction of BSP3 from 15 to 13 kDa in mass, which occurs during incubation of sperm under mild capacitating conditions, was enhanced by cryopreservation. We concluded that freezing alters the BSP protein coating on sperm, which could account in part for reduced fertility of cryopreserved semen samples.

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“…Sin duda alguna, las fallas en la manipulación de la pajilla afectan la funcionalidad espermática causando un efecto deletéreo sobre la función del acrosoma (39, 40) y de la membrana plasmática, potencial de membrana mitocondrial e integridad del ADN (41). Los espermatozoides sometidos a injuria están sujetos a una serie de alteraciones en su estructura, tales como dilatación y rompimiento a nivel de la membrana, con cambios de permeabilidad en esta y cambios en la estructura de fosfolípidos y proteínas, que traen como consecuencia una reducción en su motilidad y viabilidad espermáticas (42,43).…”

Section: Figuraunclassified

Efecto de la manipulación del semen criopreservado de bovinos Bos Taurus sobre la integridad espermática

et al. 2016

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ResumenEn el estudio se evaluó el efecto de descongelar y aplicar semen de bovinos Bos Taurus en 33 ganaderías del Magdalena Medio colombiano, y se estudió in vitro el efecto de la injuria encontrada sobre la integridad de las membranas espermáticas. La información en fincas se recopiló mediante formulario específico, mientras que el estudio in vitro se ejecutó en el laboratorio de Biotecnología Reproductiva Animal del Instituto Universitario de la Paz (Barrancabermeja, Santander). El estudio consistió en someter pajillas comerciales de 0.5 ml de toros Holstein y Pardo Suizo a la técnica convencional y a tres modificaciones de esta (injurias) mediante un diseño randomizado. Ninguna de las fincas evaluadas aplicó correctamente la práctica de la inseminación artificial; errores notorios fueron: exceso de tiempo durante la extracción de la pajilla, descongelación en la región axilar y no combinación correcta entre tiempo y temperatura. Los resultados evidenciaron diferencia significativa (P<0.05) por efecto de la raza para la integridad y resistencia de las membranas espermáticas, para la integridad de las membranas por efecto de los tratamientos cuando la pajilla se descongeló a temperatura corporal en la región axilar y para la integridad de la membrana acrosomal cuando la extracción de la pajilla se realizó en forma incorrecta. El semen de la raza Holstein evidencia una ligera tendencia a ser más resistente que el de la raza Pardo Suizo.

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Fertility-associated proteins in Nelore bull sperm membranes

Cited by 46 publications

References 27 publications

Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes

Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Efecto de la manipulación del semen criopreservado de bovinos Bos Taurus sobre la integridad espermática

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