Fertility-associated antigen on bull sperm indicates fertility potential.

Bellin, M.E.; Oyarzo, Janice; Hawkins, H. E.; Zhang, Huanmin; Smith, Roger; Forrest, D.W.; Sprott, L. R.; Ax, R. L.

doi:10.2527/1998.7682032x

Cited by 78 publications

(55 citation statements)

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“…Absence or presence of a particular HBP in the seminal plasma may be associated with fertility potential of a bull. Presence of 31 kDa HBP (fertility associated antigen) showed better conception in cattle bull has been reported [25]. In the present study also, a 31 kDa HBP was more intensely present in bull no.…”

Section: Hbps Concentration and Electrophoretic Profilesupporting

confidence: 74%

Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen

Ramteke¹,

Shende²,

Rajoriya³

et al. 2014

Vet World

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Aim:The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull. Materials and Methods:Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of ≥3+ were used for the study and categorized into three groups (Group I-Mass motility 3+, Group II-Mass motility 4+ and Group III-Mass motility 5+). Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry's method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls. Conclusion: TSPP and HBPs shows variation in concentration with respect to initial semen quality. Furthermore, presence of fertility related 31 kDa HBPs in one of the bull may be an indication of high fertility of a bull. In future, in-vivo and in-vitro correlative study on larger basis is needed for the establishment of fertility-related HBPs in semen which might establish criteria for selection of buffalo bull with high fertility potential. Results

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Section: Hbps Concentration and Electrophoretic Profilesupporting

confidence: 74%

Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen

Ramteke¹,

Shende²,

Rajoriya³

et al. 2014

Vet World

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show abstract

“…HBPs originate from the accessory sex glands and modulate heparin-sperm interactions that are indicative of fertility. Presence of three molecular weight HBP variants on sperm was previously associated with increased fertility potential of bulls (Bellin et al, 1996 and1998) and a targeted mutation in tudor domain-containing protein 1 led to male sterility because of postnatal spermatogenic defects (Chuma et al, 2006). Specifically, HBPs have been shown to play vital roles in spermatozoon survival, induction of sperm capacitation and the overall fertilization process, and any alteration in these proteins can be directly related to infertility (Miller et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Comparative testis proteome of cattleyak from different developmental stages

Sun

Mipam

Zhao

et al. 2017

Animal

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Cattleyak (hybrid of cattle and yak) exhibit higher capability in adaptability and production than cattle and yak, while the infertility of F1 males greatly restricts the effective utilization of this hybrid and little progress has been made on investigating the mechanisms of the cattleyak infertility. Cattleyak individuals at three development stages (10, 12 and 14-month old) were sampled in this work and the isobaric tag for relative and absolute quantification method was employed to identify differences between their testicular proteomes. The proteomic analysis identified 318 proteins differentially expressed with significance at 12-month stage and 327 at 14-month compared with 10-month stage, respectively. Compared with the testicular proteome from 10-month cattleyak, the gene ontology (GO) annotations of the differentially expressed proteins at 12 months did not indicate significant differences from those at 14 months, which confirmed the histological observation that germ cell reduction was more obvious and spermatogenic arrest may become more serious in 12-month-old cattleyak. On the other hand, 56 differentially expressed proteins were coexpressed at 12 and 14-month stage compared with 10-month stage, in which 32 proteins were upregulated and 24 downregulated. GO analysis revealed that most of the differently expressed proteins were involved in the molecular function of catalytic activity, transporter activity, oxidoreductase activity and protein binding. Further analysis indicated that the differently expressed proteins including testis-expressed protein 101 precursor, RNA-binding motif protein, X chromosome, putative RNA-binding protein 3, heparin-binding proteins, tudor domain-containing protein 1, glutathione S-transferases (GSTA2, GSTP1), heat shock-related 70 kDa protein 2, estradiol 17-β-dehydrogenase11, 2,4-dienoyl-CoA reductase and peroxiredoxin-2 were possibly associated with testis development and spermatogenesis, which could be selected as candidate proteins in future study to examine the mechanisms of cattleyak infertility.

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“…Other studies have identified a seminal fluid, 30-kDA heparin-binding protein as a marker of bull fertility. This protein binds to sperm and was first named "Fertility Associate Antigen" and later identified as a DNAse I like protein (Bellin et al, 1996;Bellin et al, 1998). …”

Section: Discussionmentioning

confidence: 99%