Fertile transgenic rice plants regenerated from transformed protoplasts

Shimamoto, Ko; Terada, Rie; Izawa, Takeshi; Fujimoto, Hideya

doi:10.1038/338274a0

Cited by 505 publications

(172 citation statements)

References 21 publications

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“…In pCPsyH-and other pGtlPsyH-transformed lines, the amounts of the protein present may be too low to produce an immunoreactive signal. In the former case, this would be in accordance with the generally low expression level of the CaMV 35S promoter in rice endosperm tissue (Shimamoto et aL, 1989). Seeds of several pGtlPsyH-transformed lines that did not contain immunodetectable phytoene synthase produced considerable amounts of phytoene, as did two of the pCPsyH-transformed lines.…”

Section: Al 1995)supporting

confidence: 60%

Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis

et al. 1997

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SummaryRice (Oryza aativa L.), the major food staple for more than two billion people, contains neither J~-carotene (provitamin A) nor C40 carotenoid precursors thereof in its endosperm. To improve the nutritional value of rice, genetic engineering was chosen as a means to introduce the ability to make I~-carotene into rice endosperm tissue. Investigation of the biochemical properties of immature rice endosperm using [14C]-Iabelled substrates revealed the presence of geranyl geranyl diphosphate, the C20 general isoprenoid precursor necessary for C4o carotenoid biosynthesis. Phytoene synthase, which condenses two molecules of geranyl geranyl diphosphate, is the first of four specific enzymes necessary for [~-carotene biosynthesis in plants. Therefore, the Japonica rice model variety Taipei 309 was transformed by microprojectile bombardment with a cDNA coding for phytoene synthase from daffodil {Narcissus pseudonarcissus) under the control of either a constitutive or an endosperm-specific promoter. In transgenic rice plants, the daffodil enzyme is active, as measured by the in vivo accumulation of phytoene in rice endosperm. Thus, it is demonstrated for the first time that it is in principle possible to engineer a critical step in provitamin A biosynthesis in a non-photosynthetic, carotenoid-lacking plant tissue. These results have important implications for longterm prospects of overcoming worldwide vitamin A deficiency.

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Section: Al 1995)supporting

confidence: 60%

Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis

et al. 1997

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“…To determine whether the rifa-1 gene is expressed in a cell-type-specific manner, we examined the temporal and spatial expression pattern directed by its promoter. Transgenic rice plants carrying a rifa-1-GUS transgene were generated by protoplast transformation (Shimamoto et al, 1989). Gel blot analysis of genomic DNA confirmed two independent transgenic plants, 3-4 and 10-1 (data not shown).…”

Section: The Rita-7 Gene 1s Expressed In Aleurone Cellsmentioning

confidence: 99%

“…Transgenic rice carrying a rita-7-GUS transgene was prepared as described previously (Shimamoto et al, 1989). Histochemical analysis of GUS activitywas performed as described by Jefferson et al (1987).…”

Section: Histochemical Gus Analysismentioning

confidence: 99%

The rice bZIP transcriptional activator RITA-1 is highly expressed during seed development.

et al. 1994

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Systematic protein-DNA binding studies have shown that plant basic leucine zipper (bZIP) proteins exhibit a differential binding specificity for ACGT motifs. Here, we show that the rice transcription activator-1 (RITA-1) displays a broad binding specificity for palindromic ACGT elements, beíng able to bind A-, C-, and G-box but not T-box elements. By using gel mobility shift assays with probes differing in sequences flanking the hexameric core, we identified high-affinity A-, C-, and G-box binding sites. Quantitative and competition DNA binding studies confirmed RITA-1 specificity for these sites. Using rice protoplasts as a transient expression system, we demonstrated that RITA-1 can transactivate reporter genes possessíng high-affinity but not low-affinity RITA-1 binding sites. Our results established a direct relationship between in vivo transactivation and in vitro binding actívity. Transient expression assays that demonstrated the ability of RITA-1 to transactivate a construct containing rita-7 Sflanking sequences suggest that the factor may be autoregulated. Histochemical analysis of transgenic rice plants showed that a rifa-7-P-glucuronidase transgene is expressed in aleurone and endosperm cells of developing rice seeds. We propose that RITA-1 plays a role in the regulation of rice genes expressed in developing rice seeds.

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“…What's more, the transformation system in rice is efficient (Shimamoto et al, 1989;Hiei et al 1994;Song et al, 1995). Then, molecular genetic maps in rice are dense (Causse et al, 1994;Nagamura et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation of Rice Populations Estimated Using nrDNA ITS Region Sequence

Wang¹,

Hong

2014

Korean Journal of Plant Resources

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-The rice belonging to Oryza sativa is not only has significant economic importance, for it is the major source of nutrition for about 3 billion all around the world. But also plays a vital role as a model organism, because it has a number of advantages to be a model plant, such as efficient transformation system and small genome size. Many methods and techniques have been conducted to attempt to distinguish different Oryza sativa species, such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and so on. However, studies using sequence analysis of internal transcribed spacer (ITS), a region of ribosomal RNA has not been reported until now. This study was undertaken with an aim to understand the phylogenetic relationships among sixteen isolates of Oryza sativa collected from abroad and fifteen isolates collected from Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences to compare the phylogeny relationships among different Oryza sativa species. The size variation obtained among sequenced nuclear ribosomal DNA (nrDNA) ITS region ranged from 515bp to 1000bp. The highest interspecific genetic distance (GD) was found between Sfejare 45 (FR12) and Anapuruna (FR15). Taebong isolate showed the least dissimilarity of the ITS region sequence with other thirty isolates. This consequence will help us further understanding molecular diversification in intra-species population and their phylogenetic analysis.

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Fertile transgenic rice plants regenerated from transformed protoplasts

Cited by 505 publications

References 21 publications

Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis

Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis

The rice bZIP transcriptional activator RITA-1 is highly expressed during seed development.

Genetic Variation of Rice Populations Estimated Using nrDNA ITS Region Sequence

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