Fertile Solanum tuberosum+S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum

Laferriere, L. T.; Helgeson, John P.; Allen, Caitilyn

doi:10.1007/s001220051193

Cited by 78 publications

(49 citation statements)

References 15 publications

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“…Kim et al [75] produced somatic hybrids between S. commersonii and S. tuberosum by electrofusion. Laferriere et al [81] reported that somatic hybrid plants were vigorous, their BW resistance level was similar to S. commersonii and were also male and female fertile. However, stability of resistance under different field conditions and temperature regimes remain unknown.…”

Section: Somatic Hybridizationmentioning

confidence: 99%

“…It possesses many desirable traits, including tolerance to low temperatures reported as early as 1930 [123] and resistance to nematode Ditylenchus destructor, fungus Alternaria solani, bacterial pathogen such as Rs and potato viruses X and Y [81]. Leaf extracts of several Uruguayan S. commersonii accessions collected from different geographical locations were shown to produce an inhibitory effect on the growth of Rs suggesting the presence of constitutive compounds associated with resistance [137].…”

Section: Solanum Phurejamentioning

confidence: 99%

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Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

2012

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Bacterial wilt (BW) of potato caused by the bacterium Ralstonia solanacearum (Rs) is considered a serious problem particularly in tropical, subtropical and warm temperate regions. Chemical-, cultural-and biological control of BW has limited success. Thus, the control of BW through resistance breeding and biotechnology is considered to be very important and necessary. Rs is considered a 'species complex' and has significant variation at physiological, serological and genetic levels. The bacterium has an unusually wide host range with over 400 hosts belonging to more than 50 botanical families. A large number of Solanum species have been screened for resistance to this bacterium, but so far no Solanum species has been found to have complete immunity. A high degree of resistance to Rs was found only in S. phureja, a diploid relative of cultivated tetraploid potatoes. The resistance has been transferred from S. phureja to cultivated potatoes through introgression breeding as well as somatic hybridization. Although moderate to highly resistant potato varieties have been released, high frequency of latent infection in tubers is still a major problem. Further, the resistant cultivars are not adapted to different agro-climatic zones and are not effective against all the strains of the pathogen. Biotechnological approaches involving the use of antimicrobial peptides, plant defence genes and plant resistance genes are being tried. This paper reviews the global situation with regard to screening of genetic resources and their utilization in resistance breeding for BW in potato and also the status and the opportunities that biotechnology offers to combat this disease.

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Section: Somatic Hybridizationmentioning

confidence: 99%

Section: Solanum Phurejamentioning

confidence: 99%

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

2012

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“…It grows wild country-wide and it is endemic in Uruguay, southern Brazil and the provinces of Entre Rios and Buenos Aires in Argentina. This local species has received special attention due to its resistance to frost and to some local pests (16,17). Furthermore, it is known that potato (S. tuberosum) tubers contain a lectin that has been well characterized (18)(19)(20) and has been suggested to be possibly involved in the defense mechanism of the plant.…”

Section: Purification Of the Solanum Commersonii Tuber Lectinmentioning

confidence: 99%

Screening for carbohydrate-binding proteins in extracts of Uruguayan plants

Plá

Alonso

Batista‐Viera

et al. 2003

Braz J Med Biol Res

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The presence of carbohydrate-binding proteins, namely lectins, ß-galactosidases and amylases, was determined in aqueous extracts of plants collected in Uruguay. Twenty-six extracts were prepared from 15 Uruguayan plants belonging to 12 Phanerogam families. Among them, 18 extracts caused hemagglutination (HAG) that was inhibited by mono-and disaccharides in 13 cases, indicating the presence of lectins. The other 8 extracts did not cause any HAG with the four systems used to detect HAG activity (rabbit and mouse red cells, trypsin-treated rabbit and mouse red cells). For the extracts prepared from Solanum commersonii, HAG activity and HAG inhibition were similar for those prepared from tubers, leaves and fruits, with the chitocompounds being responsible for all the inhibitions. Purification of the S. commersonii tuber lectin was carried out by affinity chromatography on asialofetuin-Sepharose, and SDS-PAGE under reducing conditions gave a single band of M r of approximately 80 kDa. The monomer N-acetylglucosamine did not inhibit HAG induced by the purified lectin, but chitobiose inhibited HAG at 24 mM and chitotriose inhibited it at 1 mM. ß-Galactosidase activity was detected in leaves and stems of Cayaponia martiana, and in seeds from Datura ferox. Only traces of amylase activity were detected in some of the extracts analyzed. The present screening increases knowledge about the occurrence of carbohydrate-binding proteins present in regional plants.

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“…This species harbours resistances to various biotic stresses such as Phytophtora infestans (Micheletto et al 2000), Ralstonia solanacerum (González et al 2013) and other severe potato pathogens, such as Pectobacterium, Verticillium, Alternaria, and X and Y viruses (Laferriere et al 1999;Carputo et al 2000). Additionally, S. commersonii has long received attention because of its frost tolerance and cold acclimation capacity (Palta and Li 1979;Palta and Simon 1993;Vega et al 2000), and it is therefore an outstanding source for broadening the genetic base of cold and drought adaptation (Chen et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, S. commersonii has long received attention because of its frost tolerance and cold acclimation capacity (Palta and Li 1979;Palta and Simon 1993;Vega et al 2000), and it is therefore an outstanding source for broadening the genetic base of cold and drought adaptation (Chen et al 1999). A few accessions of this species have been used in potato introgression breeding (Bamberg et al 1994;Carputo et al 1997Carputo et al , 2000Carputo et al , 2009Laferriere et al 1999;Chen et al 1999). However, interactions at the cytogenetic level have not been described and thus the efficiency of introgression is not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids

et al. 2017

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Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F 1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii 9 potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato.

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Fertile Solanum tuberosum+S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum

Cited by 78 publications

References 15 publications

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

Screening for carbohydrate-binding proteins in extracts of Uruguayan plants

Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids

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