Fertile approaches to dissecting mechanisms of premating and postmating prezygotic reproductive isolation

Moyle, Leonie C.; Jewell, Cathleen P; Kostyun, Jamie L.

doi:10.1016/j.pbi.2013.12.005

Cited by 29 publications

(27 citation statements)

References 64 publications

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“…Moreover, it is clear that pollen tube-pistil interactions represent a key prezygotic barrier to interspecific hybridization; however, few molecules have been identified that facilitate sperm delivery in intraspecific pollinations, while preventing sperm delivery in interspecific pollinations (Moyle et al, 2014). Determination of the pollen tube and pistil transcriptomes during pollination would facilitate identification of patterns of gene expression required for successful fertilization; however, this has been technically challenging because of the way that pollen tubes grow within complex pistil tissue.…”

Section: Discussionmentioning

confidence: 99%

The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing

et al. 2017

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Section: Discussionmentioning

confidence: 99%

The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing

et al. 2017

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“…Just as in animals, flowering plant postmating prezygotic reproductive isolation operates after mating (in this case, after transfer of pollen between species) but before fertilization; these barriers are due to the reduced effectiveness of heterospecific male-female interactions or, in some cases, because of active heterospecific rejection mechanisms (reviewed in Moyle et al 2014). In this study, we focus on postmating isolating barriers among plant species that manifest after the male pollen grain germinates to produce a pollen tube (the haploid male gametophyte), while it is growing down through the female pistil (the diploid maternal floral reproductive tract composed of the stigma and style) and into the ovary (Fig.…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms of postmating prezygotic reproductive isolation uncovered by transcriptome analysis

et al. 2016

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Little is known about the physiological responses and genetic mutations associated with reproductive isolation between species, especially for postmating prezygotic isolating barriers. Here, we examine changes in gene expression that accompany the expression of 'unilateral incompatibility' (UI)-a postmating prezygotic barrier in which fertilization is prevented by gamete rejection in the reproductive tract [in this case of pollen tubes (male gametophytes)] in one direction of a species cross, but is successful in the reciprocal crossing direction. We use whole-transcriptome sequencing of multiple developmental stages of male and female tissues in two Solanum species that exhibit UI to: (i) identify transcript differences between UI-competent and UI noncompetent tissues; (ii) characterize transcriptional changes specifically associated with the phenotypic expression of UI; and (iii) using these comparisons, evaluate the behaviour of a priori candidate loci for UI and identify new candidates for future manipulative work. In addition to describing transcriptome-wide changes in gene expression that accompany this isolating barrier, we identify at least five strong candidates for involvement in postmating prezygotic incompatibility between species. These include three novel candidates and two candidates that are strongly supported by prior developmental, functional, and quantitative trait locus mapping studies. These latter genes are known molecular players in the intraspecific expression of mate choice via genetic self-incompatibility, and our study supports prior evidence that these inter-and intraspecific postmating prezygotic reproductive behaviours share specific genetic and molecular mechanisms.

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“…The fact that sexual selection can take place after mating (postcopulatory sexual selection) is now well recognized (Parker 1970; Eberhard 1996). Phenotypes that rapidly evolve due to these forces can underlie PMPZ reproductive isolation across a wide range of animal and plant taxa (Howard et al 2009; Moyle et al 2014). …”

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confidence: 99%

Multiple Genes Cause Postmating Prezygotic Reproductive Isolation in theDrosophila virilisGroup

Ahmed-Braimah

2016

G3 Genes|Genomes|Genetics

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Understanding the genetic basis of speciation is a central problem in evolutionary biology. Studies of reproductive isolation have provided several insights into the genetic causes of speciation, especially in taxa that lend themselves to detailed genetic scrutiny. Reproductive barriers have usually been divided into those that occur before zygote formation (prezygotic) and after (postzygotic), with the latter receiving a great deal of attention over several decades. Reproductive barriers that occur after mating but before zygote formation [postmating prezygotic (PMPZ)] are especially understudied at the genetic level. Here, I present a phenotypic and genetic analysis of a PMPZ reproductive barrier between two species of the Drosophila virilis group: D. americana and D. virilis. This species pair shows strong PMPZ isolation, especially when D. americana males mate with D. virilis females: ∼99% of eggs laid after these heterospecific copulations are not fertilized. Previous work has shown that the paternal loci contributing to this incompatibility reside on two chromosomes, one of which (chromosome 5) likely carries multiple factors. The other (chromosome 2) is fixed for a paracentric inversion that encompasses nearly half the chromosome. Here, I present two results. First, I show that PMPZ in this species cross is largely due to defective sperm storage in heterospecific copulations. Second, using advanced intercross and backcross mapping approaches, I identify genomic regions that carry genes capable of rescuing heterospecific fertilization. I conclude that paternal incompatibility between D. americana males and D. virilis females is underlain by four or more genes on chromosomes 2 and 5.

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Fertile approaches to dissecting mechanisms of premating and postmating prezygotic reproductive isolation

Cited by 29 publications

References 64 publications

The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing

The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing

Molecular mechanisms of postmating prezygotic reproductive isolation uncovered by transcriptome analysis

Multiple Genes Cause Postmating Prezygotic Reproductive Isolation in theDrosophila virilisGroup

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