Ferruginol Diterpenoid Selectively Inhibits Human Thyroid Cancer Growth by Inducing Mitochondrial Dependent Apoptosis, Endogenous Reactive Oxygen Species (ROS) Production, Mitochondrial Membrane Potential Loss and Suppression of Mitogen-Activated Protein Kinase (MAPK) and PI3K/AKT Signaling Pathways

Luo, Guoqing; Zhou, Jingjing; Li, Guanjie; Hu, Ningdong; Xu, Xia; Zhou, Haibo

doi:10.12659/msm.914348

Cited by 12 publications

(10 citation statements)

References 18 publications

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“…[ 55 ] Jiang et al [ 56 ] demonstrated that natural products could exert antitumor effect via regulating ERK/MAPK signaling pathway. Luo et al [ 57 ] suggested that human thyroid cancer cells MDA‐T32, when treated with ferruginol diterpenoid could induce apoptosis and mitochondrial membrane potential loss by suppressing MAPK signaling pathway. In this study, the phosphorylation level of MAPK component protein in Rh2‐treated cells was prominently increased in a dose‐dependent manner, indicating that Rh2 promoted the MAPK pathway in U20S cells.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rh2 impedes proliferation and migration and induces apoptosis by regulating NF‐κB, MAPK, and PI3K/Akt/mTOR signaling pathways in osteosarcoma cells

Gao

Feng

et al. 2020

J Biochem & Molecular Tox

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Ginsenoside Rh2 is a primary bioactive compound obtained from ginseng that indicated anticancer activities against several malignant tumors. However, previous studies have reported little about the inhibitory effect of Rh2 on osteosarcoma (OS). This study aims to explore whether Rh2 could exert anticancer effects in OS cells and further investigate the proliferation, migration, and apoptosis mechanisms induced by Rh2 in human OS U20S cell line. The viability of U20S cells was obtained by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Cell migration property was analyzed by wound-healing assay. Apoptosis was visualized using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), 4′, 6-diamidino-2-phenylindole (DAPI), and annexin V/propidium iodide (PI) staining. Relative protein expressed was confirmed through Western blot analysis. Mitochondrial membrane potential was evaluated by JC-1 staining. In this study, we used broad-spectrum anticancer drug cisplatin (CP) as a positive control. The results indicated that Rh2 remarkably inhibited cell viability of U20S cells in a dose-and time-dependent manner, and suppressed migration. TUNEL, DAPI, annexin V/PI, and JC-1 assay suggested that Rh2 could induce cellular apoptosis. Rh2 could reduce the levels of Bcl-2, caspase 3, and caspase 9, and promote the expression level of Bax in U20S cells. Moreover, Rh2 could induce apoptosis by promoting mitogen-activated protein kinase (MAPK) signaling pathway and inhibit PI3K/Akt/mTOR and nuclear factor-κB (NF-κB) signaling pathway in U20S cells. These findings indicated that Rh2 has an anticancer effect on U20S cells by regulating MAPK, PI3K/Akt/mTOR, and NF-κB signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Ginsenoside Rh2 impedes proliferation and migration and induces apoptosis by regulating NF‐κB, MAPK, and PI3K/Akt/mTOR signaling pathways in osteosarcoma cells

Gao

Feng

et al. 2020

J Biochem & Molecular Tox

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“…The anticancer activity of these diterpenoids have been attributed to their potential to induce reactive oxygen species (ROS), alteration of MMP, apoptosis, and cell cycle arrest 13 . A number of diterpenoids has been evaluated in cell line as well as in animal model and even in clinical trials for their anticancer activity.…”

Section: Discussionmentioning

confidence: 99%

Kirenol inhibited the cell survival and induced apoptosis in human thyroid cancer cells by altering PI3K/AKT and MAP kinase signaling pathways

Wang

Alarifi

et al. 2020

Environmental Toxicology

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The thyroid cancer, especially papillary thyroid cancers are very common among population with high intake of iodine or iodine uptake. Even though several treatment options are available, there is still complication and side effects are still persistent. The role of signaling molecules in cancer signaling is very vast and their significance in progression of disease was increasing which leads to mortality of the patient. The major key players are PI3K, AKT and MAP kinase, involves in cell survival, proliferation, and inhibition of apoptosis and are the promising candidate for cancer treatment target, several researchers focuses these molecule to treat various acute and chronic diseases like cancer. On the other side, various literatures propose that natural compounds derived from plant source are shown potent anticancer property against several cancers. In our study we are looking in to one such active principle obtained from plant source, a diterpenoid compound kirenol, and its role thyroid cancer. Here, we report that kirenol role on various cellular mechanisms like induction of apoptosis, enhancing ROS indirectly by inhibiting antioxidants, altering the signaling mechanism of cell survival and apoptosis. Our study proposes that kirenol involved in the cancer cell cytotoxicity by inducing apoptosis and inhibition of cancer cell survival. Thus, targeting this signaling molecule with kirenol definitely favors and may lead to a therapeutic modality for thyroid cancer.

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“…It is well established that the PI3K/AKT pathway regulates inflammation and cell survival [21,22]. Activation of the PI3K/AKT pathway has been reported to induce inflammation and apoptosis of chondrocyte [23].…”

Section: Discussionmentioning

confidence: 99%

Salidroside Suppresses IL-1β-Induced Apoptosis in Chondrocytes via Phosphatidylinositol 3-Kinases (PI3K)/Akt Signaling Inhibition

Wang

et al. 2019

Med Sci Monit

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Background Salidroside, a natural dietary isothiocyanate, has been widely studied for its multiple effects, including promoting proliferation, anti-inflammation, and anti-apoptosis. In the present study, these effects of Salidroside were explored to assess whether it could prevent osteoarthritis (OA) in vitro . Material/Methods The cytotoxic and proliferating effects of Salidroside on chondrocytes were detected by use of the Cell Counting Kit 8 assay. The expression levels of proteins were detected by Western blot. The cell apoptosis level was assessed by flow cytometry, and the levels of ROS, NO, caspase 3, and caspase 9 were assessed to evaluate the level of apoptosis. The expression level of pro-inflammatory factors was detected by ELISA. Results Our results demonstrated that Salidroside promotes chondrocytes proliferation, inhibits IL-1β-induced apoptosis and inflammation, and scavenges reactive oxygen species (ROS) and NO of chondrocytes. Salidroside upregulates the level of Bcl-2 and downregulates the level of Bax. Salidroside also inhibits the production of caspase 3/9 and suppresses the phosphorylation of PI3K and AKT. Conclusions Our results suggest that Salidroside prevents OA by its powerful pro-proliferating, anti-phlogistic, and anti-apoptotic effects by inhibiting PI3K/AKT.

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Ferruginol Diterpenoid Selectively Inhibits Human Thyroid Cancer Growth by Inducing Mitochondrial Dependent Apoptosis, Endogenous Reactive Oxygen Species (ROS) Production, Mitochondrial Membrane Potential Loss and Suppression of Mitogen-Activated Protein Kinase (MAPK) and PI3K/AKT Signaling Pathways

Cited by 12 publications

References 18 publications

Ginsenoside Rh2 impedes proliferation and migration and induces apoptosis by regulating NF‐κB, MAPK, and PI3K/Akt/mTOR signaling pathways in osteosarcoma cells

Ginsenoside Rh2 impedes proliferation and migration and induces apoptosis by regulating NF‐κB, MAPK, and PI3K/Akt/mTOR signaling pathways in osteosarcoma cells

Kirenol inhibited the cell survival and induced apoptosis in human thyroid cancer cells by altering PI3K/AKT and MAP kinase signaling pathways

Salidroside Suppresses IL-1β-Induced Apoptosis in Chondrocytes via Phosphatidylinositol 3-Kinases (PI3K)/Akt Signaling Inhibition

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