Ferrtin secretion by human mononuclear cells: Association with HLA phenotype

Pollack, Marilyn S.; Silva, Ana Martins; Moshief, Rebecca D.; Groshen, Susan; Bognacki, John; Dupont, Bo; Sousa, Maria de

doi:10.1016/0090-1229(83)90061-2

Cited by 21 publications

(6 citation statements)

References 34 publications

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“…Activated T lymphocytes express transferrin receptors (Pattanapanyasat and Hoy 1991). Both activated and non-activated T lymphocytes synthesize ferritin but do not secrete (Dorner et al 1980;Pattanapanyasat and Hoy 1991;Pollack et al 1983). Lymphocytes could therefore act as a "mobile" and easily "mobilizable" iron-storage compartment protecting from iron-mediated toxicity, as originally postulated by De Sousa (1978).…”

Section: Discussionmentioning

confidence: 93%

Co-selection of the H63D mutation and the HLA-A29 allele: a new paradigm of linkage disequilibrium?

Cardoso¹,

Alves

Mascarenhas³

et al. 2002

Immunogenetics

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The major histocompatibility complex (MHC) shows a remarkable conservation of particular HLA antigens and haplotypes in linkage disequilibrium in most human populations, suggesting the existence of a convergent evolution. A recent example of such conservation is the association of particular HLA haplotypes with the HFE mutations. With the objective of exploring the significance of that association, the present paper offers an analysis of the linkage disequilibrium between HLA alleles or haplotypes and the HFE mutations in a Portuguese population. Allele and haplotype associations between HLA and HFE mutations were first reviewed in a population of 43 hemochromatosis families. The results confirmed the linkage disequilibrium of the HLA haplotype HLA-A3-B7 and the HLA-A29 allele, respectively, with the HFE mutations C282Y and H63D. In order to extend the study of the linkage disequilibrium between H63D and the HLA-A29-containing haplotypes in a normal, random population, an additional sample of 398 haplotypes was analyzed. The results reveal significant linkage disequilibrium between the H63D mutation and all HLA-A29-containing haplotypes, favoring the hypothesis of a co-selection of H63D and the HLA-A29 allele itself. An insight into the biological significance of this association is given by the finding of significantly higher CD8 + T-lymphocyte counts in subjects simultaneously carrying the H63D mutation and the HLA-A29 allele.

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Section: Discussionmentioning

confidence: 93%

Co-selection of the H63D mutation and the HLA-A29 allele: a new paradigm of linkage disequilibrium?

Cardoso¹,

Alves

Mascarenhas³

et al. 2002

Immunogenetics

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“…On the other hand, an indirect mechan ism of liver damage can be hypothesized, since free oxygen radicals have been de scribed to interfere in vitro with lympho cyte function and rosette formation [43,44], Altered functions of T lymphocytes might favor a longer viral persistence and/or prime autoimmunity phenomena. In the patients of our study T-cell subsets did not show alterations.…”

Section: Discussionmentioning

confidence: 99%

Changes in Superoxide Anion Production in Neutrophils from Multitransfused β-Thalassemia Patients: Correlation with Ferritin Levels and Liver Damage

et al. 1984

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Superoxide anion production in resting and PMA- or zymosan-stimulated neutrophils was evaluated in 21 β-thalassemia patients. The results were correlated with ferritin values, hepatitis B virus serum markers, liver pathology, immunoglobulin levels and T-cell subsets. Superoxide anion generation from resting or PMA-stimulated neutrophils was significantly higher in patients than in controls. On the contrary, zymosan-stimulated neutrophils showed reduced superoxide anion production. Increased superoxide anion production in resting neutrophils showed a significant correlation to the values of ferritin. In addition, patients with biopsy-proven chronic liver disease showed significantly increased ferritin levels and superoxide anion production as compared to the remaining patients. No correlation was observed between superoxide anion production and the presence or the absence of hepatitis B virus serum markers, immunoglobulin levels and T-cell subsets. A possible role of interreactions between iron and oxygen radicals in determining liver damage in β-thalassemia patients is suggested.

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“…Monocytes also synthesize ferritin (Summers et al, 1975) which is visualized by cytoplasmic immunostaining (Summers et al, 1974;Nishiya et al, 1980). The minimal surface ferritin immunoreactivity and iron nitrilotriacetate-acid ferrocyanide and/or acid ferrocyanide positivity of monocytes in the present study could be either due to ferritin secretion (Broxmeyer et al, 1982;Pollack et al, 1983; or to the binding of plasma ferritin. Intracellular ferritin appears during the terminal differentiation of monocytes (Andreesen et al, 1984;Worwood et al, 1984), and the brilliant multigranular ferrocyanide staining of bone marrow macrophages is consistent with the known presence of cytoplasmic ferritin/haemosiderin deposits in these cells.…”

Section: Discussionmentioning

confidence: 55%

Differential staining of neutrophils and monocytes: Surface and cytoplasmic iron-binding proteins

et al. 1988

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Lactoferrin, transferrin, and ferritin were systematically visualized and semiquantified in neutrophils and monocytes/macrophages using indirect immunofluorescence and functional cytochemical techniques. They localized on cell surfaces and within the cytoplasm at the light and electron microscopical levels. In normal subjects, subpopulations of blood neutrophils and monocytes had surface lactoferrin, but little surface transferrin or ferritin was observed on these cells. Most neutrophils had brilliant granular cytoplasmic positivity for lactoferrin; variable fractions of monocytes had weak to moderate diffuse cytoplasmic lactoferrin staining localized most prominently to the cytoplasmic matrix. Most neutrophils had cytoplasmic ferritin, but few had cytoplasmic transferrin, whereas larger subpopulations of monocytes had cytoplasmic staining reactions for both proteins. To analyse maturing cells, the iron nitrilotriacetate-acid ferrocyanide method was adapted for the light microscopical analysis of neutrophils and monocytes/macrophages in soft agar culture. Further, a combined stain that visualizes iron nitrilotriacetate-acid ferrocyanide reactivity and alpha-naphthyl butyrate esterase activity in cells in blood and marrow smears was developed. The relative quantities and subcellular distribution of iron-binding proteins in neutrophils and monocytes/macrophages defined by the present methods can be correlated with biochemical, maturational, and functional properties of these cells.

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Ferrtin secretion by human mononuclear cells: Association with HLA phenotype

Cited by 21 publications

References 34 publications

Co-selection of the H63D mutation and the HLA-A29 allele: a new paradigm of linkage disequilibrium?

Co-selection of the H63D mutation and the HLA-A29 allele: a new paradigm of linkage disequilibrium?

Changes in Superoxide Anion Production in Neutrophils from Multitransfused β-Thalassemia Patients: Correlation with Ferritin Levels and Liver Damage

Differential staining of neutrophils and monocytes: Surface and cytoplasmic iron-binding proteins

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