Ferrovibrio denitrificans gen. nov., sp. nov., a novel neutrophilic facultative anaerobic Fe(II)-oxidizing bacterium

Abstract

A neutrophilic Fe(II)‐oxidizing bacterium was isolated from the redox zone of a low‐salinity spring in Krasnodar krai (Russia), at the FeS–Fe(OH)3 interface deposited at the sediment surface. The cells of strain Sp‐1 were short, thin motile vibrioids with one polar flagellum dividing by binary fission. The optimal values and ranges for pH and temperature were pH 6.2 (5.5–8) and 35 °C (5–45 °C), respectively. The organism was a facultative anaerobe. Strain Sp‐1 was capable of organotrophic, lithoheterotrophic and mixotrophic growth with Fe(II) as an electron donor. The denitrification chain was ‘disrupted’. Oxidation of Fe(II) was coupled to reduction of NO3 − to NO2 − or of N2O to N2, as well as under microaerobic conditions, with O2 as an electron acceptor. The DNA G+C content was 64.2 mol%. According to the results of phylogenetic analysis, the strain was 10.6–12% remote from the closest relatives, members of the genera Sneathiella, Inquilinus, Oceanibaculum and Phaeospirillum within the Alphaproteobacteria. Based on its morphological, physiological and taxonomic characteristics, together with the results of phylogenetic analysis, strain Sp‐1 is described as a member of a new genus Ferrovibrio gen. nov., with the type species Ferrovibrio denitrificans sp. nov. and the type strain Sp‐1T (= LMG 25817T = VKM B‐2673T).

Ferrovibrio xuzhouensis sp. nov., a cyhalothrin-degrading bacterium isolated from cyhalothrin contaminated wastewater

“…S3). Phosphatidylethanolamine (PE) was also found in F. denitrificans Sp-1 T (Sorokina et al 2012), however, the presence of phosphatidylglycerol (PG) and phosphatidylmethylethanolamine (PME) is a differentiating feature of strain LM-6 T . The predominant respiratory quinone of strain LM-6 T and F. denitrificans Sp-1 T is ubiquinone-10 (Q-10).…”

“…At the time of writing, there is only one reported species (http://www. bacterio.net/ferrovibrio.html), Ferrovibrio denitrificans (Sorokina et al 2012), in the genus Ferrovibrio.…”

“…The genus Ferrovibrio was first proposed by Sorokina et al (2012) to accommodate Gram stain-negative, non-spore-forming, motile, vibrioid, oxidase-positive and weakly catalase-positive bacteria. Ubiquinone-10 is reported as the major respiratory quinone determined in bacteria of genus.…”

“…The differential phenotypic characteristics of strain LM-6 T and F. denitrificans Sp-1 T Strains All data are from this study except for the DNA G?C content of F. denitrificans Sp-1 T , which were taken fromSorokina et al (2012) ? Positive, -negative, W weakly positive, R resistant, S sensitive…”

Microbiological investigations on the water of a thermal bath at Budapest

“…Both taxa were described from aquatic habitats, the former from a spring in India [49], while the latter one from a Russian spring [50]. In addition, members of the genera Sphingobacterium and Sphingopyxis were detected in a sulfurous well (called "Matty") in Harkány (Hungary) [2].…”