Ferrous iron uptake by Bifidobacterium bifidum var. Pennsylvanicus: The effect of metals and metabolic inhibitors

Bezkorovainy, Anatoly; Solberg, Leslie; Poch, Mark; Miller-Catchpole, Robin

doi:10.1016/0020-711x(87)90135-2

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…Interestingly, the protection of breast-fed babies by bifidobacteria in the large intestine, against colonization with potentially pathogenic bacteria, has been suggested to be partly due to the competition for Fe 2+ ions [14]. Bifidobacteria possess an ATPdependent ferrous iron uptake system and are unable to use various siderophore-Fe 3+ complexes [15,16]. The fact that the intact enterochelin system in strain AB1515-1F did not affect the poor colonization phenotype of the leo mutant shows that the limiting factor is not Fe 3+.…”

Section: Resultsmentioning

confidence: 99%

Escherichia coli K-12 ferrous iron uptake mutants are impaired in their ability to colonize the mouse intestine

Stojiljković¹,

Cobeljić

Hantke³

1993

FEMS Microbiology Letters

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The streptomycin-treated mouse colonization model was used to investigate the role of the Fe 2+ uptake system (Feo) of Escherichia coli K12 in the colonization of the mouse intestine. Mutants impaired in the uptake of Fe 2+ ions were shown to be deficient also in their colonization ability. Both enterochelin-producing and enterochelin-nonproducing Escherichia coli leo mutants were unable to colonize the mouse intestine. These results demonstrated that Fe(II) is an essential source of iron for E. coli grown in the intestine.

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Section: Resultsmentioning

confidence: 99%

Escherichia coli K-12 ferrous iron uptake mutants are impaired in their ability to colonize the mouse intestine

Stojiljković¹,

Cobeljić

Hantke³

1993

FEMS Microbiology Letters

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“…of the human being, especially in breast-fed infants (Mitsuoka 1982 ;Bezkorovainy and Miller-Catchpole 1989). They are Bacterial strains, plasmids and media considered to play an important role in the proper balance of…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of two plasmids from Bifidobacterium longum

Park

Lee

1997

Letters in Applied Microbiology

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. 1997. In order to develop a cloning vector system which can be used in Bifidobacterium sp., we screened about 100 bifidobacteria from the faeces of adults and children. Among them, only one strain, identified as B. longum KJ, was shown to contain extrachromosomal DNAs. Bifidobacterium longum KJ showed multiple plasmid DNA bands which were resolved to be multimers of two plasmids designated pKJ36 and pKJ50. These plasmids were cloned into the Escherichia coli vector pUC19 as pMS36 and pMS50, respectively, and restrictionmapped.

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“…The best-studied examples are Bifidobacterium bifidum (3), which is one of the early colonizers of the intestine in breast-fed infants. For the intracellular pathogen Legionella pneumophila, no siderophores have been found, and it is assumed that iron(III) reduced by the bacterium to iron(II) is the main source of iron (14).…”

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confidence: 99%

Characterization of the ferrous iron uptake system of Escherichia coli

1993

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Escherichia coli has an iron(II) transport system (feo) which may make an important contribution to the iron supply of the cell under anaerobic conditions. Cloning and sequencing of the iron(II) transport genes revealed an open reading frame (feoA) possibly coding for a small protein with 75 amino acids and a membrane protein with 773 amino acids (feoB). The upstream region offeoAB contained a binding site for the regulatory protein Fur, which acts with iron(ll) as a corepressor in all known iron transport systems ofE. coli. In addition, a Fnr binding site was identified in the promoter region. The FeoB protein had an apparent molecular mass of 70 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was localized in the cytoplasmic membrane.The sequence revealed regions of homology to ATPases, which indicates that ferrous iron uptake may be ATP driven. FeoA or FeoB mutants could be complemented by clones with the feoA orfeoB gene, respectively.Since iron(III) is practically insoluble at neutral pH, many aerobic microorganisms secrete siderophores, iron(III) chelating compounds, for their iron supply. Six different siderophore-iron(III) transport systems in Escherichia coli have been sequenced and analyzed, and many more in other gram-negative bacteria have been characterized (4). These transport systems share a common structure. A ferric siderophore-specific receptor in the outer membrane delivers its substrate in an energy-dependent mechanism to the periplasm. The energy is provided by the TonB-ExbB-ExbD complex (4). Even heme (33) and transferrin iron (6) are taken up in a TonB-dependent manner. With

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Ferrous iron uptake by Bifidobacterium bifidum var. Pennsylvanicus: The effect of metals and metabolic inhibitors

Cited by 13 publications

References 10 publications

Escherichia coli K-12 ferrous iron uptake mutants are impaired in their ability to colonize the mouse intestine

Escherichia coli K-12 ferrous iron uptake mutants are impaired in their ability to colonize the mouse intestine

Isolation and characterization of two plasmids from Bifidobacterium longum

Characterization of the ferrous iron uptake system of Escherichia coli

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