Ferrostatin‑1 alleviates liver injury via decreasing ferroptosis following ricin toxin poisoning in rat

Lin, Ruijiao; Jia, Zijie; Chen, Hongbing; Xiong, Hongli; Bian, Cunhao; He, Xin; Wei, Bi; Fu, Junfeng; Zhao, Minzhu; Li, Jianbo

doi:10.1016/j.tox.2024.153767

Cited by 1 publication

(1 citation statement)

References 47 publications

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“…At present, RSL3 or erastin is known to induce ferroptosis by inhibiting the activity of glutathione peroxidase 4 (GPX4). , HeLa cells treated with RSL3 (1 μM) or erastin (10 μM), followed by the treatment with SRF-HClO (10 μM) for 0.5 h, showed strong red fluorescent signals from inside the lysosomes with feeble green fluorescent signals (Figure ), indicating that the HClO level increased significantly during the ferroptosis process. However, upon incubating the cells with ferrostatin-1 (Fer-1, a classical ferroptosis inhibitor), the fluorescence intensity of the probe remained basically the same as that of the probe only. , In addition, further validated by flow cytometry, the conclusion was consistent with the above (Figure S8). These results indicate that SRF-HClO has the ability to monitor dynamic HClO during the ferroptosis process.…”

Section: Resultssupporting

confidence: 77%

Ratiometric Fluorescent Probe for Super-Resolution Imaging of Lysosome HClO in Ferroptosis Cells

Zheng,

Peng,

Liu

et al. 2024

Anal. Chem.

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Ferroptosis is an iron-dependent programmed cell death that is characterized by the dysregulation of lipid reactive oxygen species (ROS) production, causing abnormal changes in hypochlorous acid (HClO) levels in lysosomes. Super-resolution imaging can observe the fine structure of the lysosome at the nanometer level; therefore, it can be used to detect lysosome HClO levels during ferroptosis at the suborganelle level. Herein, we utilize a ratiometric fluorescent probe, SRF-HClO, for super-resolution imaging of lysosome HClO. Structured-illumination microscopy (SIM) improves the accuracy of lysosome targeting and enables the probe SRF-HClO to be successfully applied to rapidly monitor the up-regulated lysosome HClO at the nanoscale during inflammation and ferroptosis. Importantly, the probe SRF-HClO can also detect HClO changes in inflammatory and ferroptosis mice and evaluate the inhibitory effect of ferroptosis on mice tumors.

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