Ferroptosis-related lncRNA model based on CFAP58-DT for predicting prognosis and immunocytes infiltration in endometrial cancer

Abstract

Background: Endometrial cancer (EC) is a kind of common gynecological tumor. Further study on the markers related to the prognosis of endometrial cancer is important for women worldwide. Methods:The Cancer Genome Atlas (TCGA) database was used to obtain the transcriptome profiling and clinical data. A model was built using packages based on R software. Immune-related databases were employed to analyze the infiltration of immunocytes. Quantitative real-time PCR (qRT-PCR), cell counting kit-8 (CCK-8), and transwell assays were utilized to investigate the role of CFAP58-DT in EC.Results: Following Cox regression analysis, 1,731 ferroptosis-related long non-coding RNA (lncRNA)were screened, and a 9-related lncRNA prognostic model was constructed. Patients were classified as highand low-risk according to their expression spectrum. Kaplan-Meier (KM) analysis showed that the prognosis of low-risk patients was poor. Operating characteristic curves, decision curve analysis, and a nomogram suggested the model could independently guide prognostic evaluation, with higher sensitivity, specificity, and efficiency than other common clinical characteristics. Gene Set Enrichment Analysis (GSEA) was conducted to determine the enriched pathways among the two groups and evaluation of the immune-infiltrating conditions were performed to help improve immune therapy. Finally, we conducted cytological studies on the model's most important indicators.Conclusions: Overall, we identified a prognostic ferroptosis-related lncRNA model based on CFAP58-DT for predicting the prognosis and immune-infiltrating conditions in EC. We concluded that the potential oncogenic role of CFAP58-DT can further guide immunotherapy and chemotherapy.

Ferroptosis, Metabolic Rewiring, and Endometrial Cancer

“…The knockdown of CFAP58-DT lncRNA decreased the viability and migratory properties of EC cells Ishikawa and HEC-1A. In accordance with the previous studies, low and high-risk EC groups differed in the degree of immune cell infiltration; for example, the number of dendritic cells and T cells was lower in the tissues of high-risk patient tumors [171].…”

Ferroptosis contributes to the progression of female-specific neoplasms, from breast cancer to gynecological malignancies in a manner regulated by non-coding RNAs: Mechanistic implications

“…They then employed Cox regression analysis to create a prognostic model for ectopic pregnancy based on these nine DEFerlncRNAs. Based on the analysis, it was found that the DEFerlncRNA prognostic model is a more effective, sensitive, and specific prognostic index in EC [ 212 ]. Furthermore, the researchers employed Gene Set Enrichment Analysis/GSEA to detect differentially expressed genes between the two groups (ecEM and euEM).…”

“…They discovered that these differentially expressed genes are functionally enriched in a set of pathways, such as the Hedgehog signaling pathway, apoptotic flux, extracellular matrix receptor interaction, and natural killer cell-mediated cytotoxicity, along with others. For the purpose of predicting the prognosis of EC and immune-infiltrating conditions, the researchers also discovered a 9-lncRNA model, which includes CFAP58-DT (CFAP58 divergent transcript) [ 212 ]. The lncRNA in the model with the highest coefficient is CFAP58-DT , and poor EC outcomes are associated with high CFAP58-DT expression.…”

“…These results imply that CFAP58-DT might contribute to EC carcinogenesis. Nonetheless, to clarify the mechanisms behind this lncRNA's effects on EC cells and to confirm its role in EC, more clinical research is necessary [ 212 ].…”