Ferroptosis-related Genes for Overall Survival Prediction in Patients with Colorectal Cancer can be Inhibited by Gallic acid

Hong, Zongchao; Tang, Peili; Liu, Bo; Ran, Chongwang; Yuan, Chong; Zhang, Ying; Lü, Yi; Duan, Xueyun; Yang, Yanfang; H, Wu

doi:10.7150/ijbs.57164

Cited by 52 publications

(37 citation statements)

References 41 publications

(42 reference statements)

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“…Zhang et al found that active ingredients of Rheum palmatum L. had anti-lung cancer effect via induction of apoptosis by applying network pharmacology method 18 . Hong et al used bio-information analysis method to found gallic acid can inhibit the growth of colon cancer cells, and its mechanism of action is related to ferroptosis 19 . In this study, we used a combination of bio-information analysis and network pharmacology, as well as molecular docking, to simulate the possible mechanisms of TSA treatment for LUAD.…”

Section: Discussionmentioning

confidence: 99%

Tanshinone IIA suppresses the progression of lung adenocarcinoma through regulating CCNA2-CDK2 complex and AURKA/PLK1 pathway

Zhang

Zhou

et al. 2021

Sci Rep

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Lung adenocarcinoma (LUAD) belongs to a subgroup of non-small cell lung cancer (NSCLC) with an increasing incidence all over the world. Tanshinone IIA (TSA), an active compound of Salvia miltiorrhiza Bunge., has been found to have anti-tumor effects on many tumors, but its anti-LUAD effect and its mechanism have not been reported yet. In this study, bio-information analysis was applied to characterize the potential mechanism of TSA on LUA, biological experiments were used to verify the mechanisms involved. TCGA, Pubchem, SwissTargetPrediction, Venny2.1.0, STRING, DAVID, Cytoscape 3.7.2, Omicshare, GEPIA, RSCBPDB, Chem Draw, AutoDockTools, and PyMOL were utilized for analysis in the bio-information analysis and network pharmacology. Our experiments in vitro focused on the anti-LUAD effects and mechanisms of TSA on LUAD cells (A549 and NCI-H1975 cells) via MTT, plate cloning, Annexin V-FITC and PI dual staining, flow cytometry, and western blot assays. A total of 64 differentially expressed genes (DEGs) of TSA for treatment of LUAD were screened out. Gene ontology and pathway analysis revealed characteristic of the DEGs network. After GEPIA-based DEGs confirmation, 46 genes were considered having significant differences. Further, 10 key DEGs (BTK, HSD11B1, ADAM33, TNNC1, THRA, CCNA2, AURKA, MIF, PLK1, and SORD) were identified as the most likely relevant genes from overall survival analysis. Molecular Docking results showed that CCNA2, CDK2 and PLK1 had the lowest docking energy. MTT and plate cloning assays results showed that TSA inhibited the proliferation of LUAD cells in a concentration-dependent manner. Annexin V-FITC and PI dual staining and flow cytometry assays results told that TSA promoted the apoptosis of the two LUAD cells in different degrees, and induced cycle arrest in the G1/S phase. Western blot results showed that TSA significantly down-regulated the expression of CCNA2, CDK2, AURKA, PLK1, and p-ERK. In summary, TSA could suppress the progression of LUAD by inducing cell apoptosis and arresting cell cycle, and these were done by regulating CCNA2-CDK2 complex and AURKA/PLK1 pathway. These findings are the first to demonstrate the molecular mechanism of TSA in treatment of LUAD combination of network bio-information analysis and biological experiments in vitro.

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Section: Discussionmentioning

confidence: 99%

Tanshinone IIA suppresses the progression of lung adenocarcinoma through regulating CCNA2-CDK2 complex and AURKA/PLK1 pathway

Zhang

Zhou

et al. 2021

Sci Rep

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“…For example, honokiol, a biphenolic compound originally extracted from Magnolia grandiflora that has been reported to exert anticancer effects through the inhibition of GPX4 and activation of ferroptosis in CRC cells [137]. Besides, gallic acid, Chinese matrine, and Chinese herb Curcumae rhizoma have also been shown to induce ferroptosis in CRC cells by inhibiting GPX4 and SCL7A11 [138][139][140]. However, the underlying mechanism by which these bioactive components regulate the expression of GPX4 and SLC7A11 is still largely unknown and require further study.…”

Section: Effects Of Dietary Nutrients and Phytochemicals On Ferroptosis And Gastric Diseasesmentioning

confidence: 99%

Ferroptosis: A Double-Edged Sword in Gastrointestinal Disease

Liu

Xiao

2021

IJMS

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Ferroptosis is a novel form of regulated cell death (RCD) that is typically accompanied by iron accumulation and lipid peroxidation. In contrast to apoptosis, autophagy, and necroptosis, ferroptosis has unique biological processes and pathophysiological characteristics. Since it was first proposed in 2012, ferroptosis has attracted attention worldwide. Ferroptosis is involved in the progression of multiple diseases and could be a novel therapeutic target in the future. Recently, tremendous progress has been made regarding ferroptosis and gastrointestinal diseases, including intestinal ischemia/reperfusion (I/R) injury, inflammatory bowel disease (IBD), gastric cancer (GC), and colorectal cancer (CRC). In this review, we summarize the recent progress on ferroptosis and its interaction with gastrointestinal diseases. Understanding the role of ferroptosis in gastrointestinal disease pathogenesis could provide novel therapeutic targets for clinical treatment.

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“…In Bai's research [23], ziyuglycoside II induced apoptosis through the accumulation of reactive oxygen species (ROS), triggered complete autophagic flux, and improved the sensitivity of 5-fluorouracil in colorectal cancer cells. Gallic acid, a major compound of phenols in Sanguisorba officinalis L., could bind to ferroptosis-related targets and regulate the expression of corresponding proteins; ferroptosis inhibitors reversed the experimental results, improving CRC by regulating ferroptosis [24]. Another compound named 3,3 ,4trimethylellagic acid (TMEA), a tannin compound isolated from Sanguisorba officinalis L., executed its anti-cancer activity by inducing apoptosis and inhibiting angiogenesis in CRC cells via the apoptotic and VEGF/PI3K/AKT/mTOR pathways [25].…”

Section: Discussionmentioning

confidence: 99%

A Bioactive Compound from Sanguisorba officinalis L. Inhibits Cell Proliferation and Induces Cell Death in 5-Fluorouracil-Sensitive/Resistant Colorectal Cancer Cells

et al. 2021

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Colorectal cancer (CRC) is one of the most common cancer in the world. The first line chemotherapeutic agent, 5-fluorouracil (5-FU), plays a predominant role in the clinical treatment of CRC. However, with the wide use of 5-FU, more and more CRC patients have been obtaining drug resistance to 5-FU, which leads to a large amount of treatment failures. One of the effective strategies to overcome this obstacle is to find bioactive natural products from traditional medicine. In our previous work, Sanguisorba officinalis L. was found to exert a strong anti-proliferative activity against 5-FU-senstive/resistant CRC cells. Therefore, several compounds were isolated from this herb and screened for their anti-CRC effects to find promising compounds. Among them, a triterpenoid compound named 3β-[(α-l-arabinopyranosyl) oxy]-urs-12,18(19)-dien-28-oic acid β-d-glucopyranosyl ester (AGE), showed strong activity against both 5-FU-senstive and resistant CRC cells. In order to further study the mechanism of AGE on CRC cells, flow cytometer analysis, mitochondrial membrane potential (MMP) measurement, Western blotting, and RT-PCR assays were performed. Results demonstrated that AGE induced cell death by apoptosis pathway and autophagy, and inhibited cell proliferation via cell cycle arrest in G0-G1 phase mediated by Wnt signaling pathway. Therefore, AGE may be a potential bioactive compound for CRC treatment in clinic.

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Ferroptosis-related Genes for Overall Survival Prediction in Patients with Colorectal Cancer can be Inhibited by Gallic acid

Cited by 52 publications

References 41 publications

Tanshinone IIA suppresses the progression of lung adenocarcinoma through regulating CCNA2-CDK2 complex and AURKA/PLK1 pathway

Tanshinone IIA suppresses the progression of lung adenocarcinoma through regulating CCNA2-CDK2 complex and AURKA/PLK1 pathway

Ferroptosis: A Double-Edged Sword in Gastrointestinal Disease

A Bioactive Compound from Sanguisorba officinalis L. Inhibits Cell Proliferation and Induces Cell Death in 5-Fluorouracil-Sensitive/Resistant Colorectal Cancer Cells

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