Ferroptosis landscape in prostate cancer from molecular and metabolic perspective

Liang, Jiaming; Liao, Yihao; Wang, Pu; Yang, Kun; Wang, Keke; Zhong, Boqiang; Zhou, Diansheng; Cao, Qian; Li, Junbo; Zhao, Yang; Jiang, Ning

doi:10.1038/s41420-023-01430-0

Cited by 4 publications

(1 citation statement)

References 162 publications

(175 reference statements)

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“…However, their efficacy diminishes over time [5]. Nonetheless, emerging evidence suggests Biomedicines 2024, 12, 661 2 of 13 that the activation of alternative oncogenic-signaling pathways plays a pivotal role in circumventing dependence on androgens during the progression of CRPC [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

YAP1 Regulates the YAP1/AR/PSA Axis through Autophagy in Castration-Resistant Prostate Cancer and Mediates T-Cell Immune and Inflammatory Cytokine Infiltration

Wang,

Wu,

et al. 2024

Biomedicines

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The emergence of castration-resistant prostate cancer (CRPC) following androgen deprivation therapy (ADT) is associated with increased malignancy and limited treatment options. This study aims to investigate potential connections between immune cell infiltration and inflammatory cytokines with the YAP1/AR/PSA axis by exploring their interactions with autophagy. Our research reveals heightened levels of Yes-associated protein 1 (YAP1) expression in CRPC tissues compared with tissues from androgen-dependent prostate cancer (ADPC) and benign prostate hyperplasia (BPH). Additionally, a correlation was observed between YAP1 and PSA expressions in CRPC tissues, suggesting that YAP1 may exert a regulatory influence on PSA expression within CRPC. Enhanced YAP1 expression in C4-2 cells resulted in the upregulation of androgen receptor (AR) nuclear translocation and intracellular prostate-specific antigen (PSA) levels. Conversely, the suppression of YAP1 led to a decrease in PSA expression, suggesting that YAP1 may positively regulate the PSA in castration-resistant prostate cancer (CRPC) by facilitating AR nuclear import. The modulation of the autophagy activity exerts a significant impact on the expression levels of YAP1, the AR, and the PSA. Moreover, recent advancements in immunity and inflammation studies present promising avenues for potential therapies targeting prostate cancer (PC).

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Section: Introductionmentioning

confidence: 99%

YAP1 Regulates the YAP1/AR/PSA Axis through Autophagy in Castration-Resistant Prostate Cancer and Mediates T-Cell Immune and Inflammatory Cytokine Infiltration

Wang,

Wu,

et al. 2024

Biomedicines

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TNIK drives castration-resistant prostate cancer via phosphorylating EGFR

Guo,

Liang,

Wang

et al. 2024

iScience

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sBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides

Garapati,

Ding,

Charlesworth

et al. 2023

Clin Proteom

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Background Cell surface proteins perform critical functions related to immune response, signal transduction, cell–cell interactions, and cell migration. Expression of specific cell surface proteins can determine cell-type identity, and can be altered in diseases including infections, cancer and genetic disorders. Identification of the cell surface proteome remains a challenge despite several enrichment methods exploiting their biochemical and biophysical properties. Methods Here, we report a novel method for enrichment of proteins localized to cell surface. We developed this new approach designated surface Biotinylation Site Identification Technology (sBioSITe) by adapting our previously published method for direct identification of biotinylated peptides. In this strategy, the primary amine groups of lysines on proteins on the surface of live cells are first labeled with biotin, and subsequently, biotinylated peptides are enriched by anti-biotin antibodies and analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Results By direct detection of biotinylated lysines from PC-3, a prostate cancer cell line, using sBioSITe, we identified 5851 peptides biotinylated on the cell surface that were derived from 1409 proteins. Of these proteins, 533 were previously shown or predicted to be localized to the cell surface or secreted extracellularly. Several of the identified cell surface markers have known associations with prostate cancer and metastasis including CD59, 4F2 cell-surface antigen heavy chain (SLC3A2) and adhesion G protein-coupled receptor E5 (CD97). Importantly, we identified several biotinylated peptides derived from plectin and nucleolin, both of which are not annotated in surface proteome databases but have been shown to have aberrant surface localization in certain cancers highlighting the utility of this method. Conclusions Detection of biotinylation sites on cell surface proteins using sBioSITe provides a reliable method for identifying cell surface proteins. This strategy complements existing methods for detection of cell surface expressed proteins especially in discovery-based proteomics approaches.

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Ferroptosis landscape in prostate cancer from molecular and metabolic perspective

Cited by 4 publications

References 162 publications

YAP1 Regulates the YAP1/AR/PSA Axis through Autophagy in Castration-Resistant Prostate Cancer and Mediates T-Cell Immune and Inflammatory Cytokine Infiltration

YAP1 Regulates the YAP1/AR/PSA Axis through Autophagy in Castration-Resistant Prostate Cancer and Mediates T-Cell Immune and Inflammatory Cytokine Infiltration

TNIK drives castration-resistant prostate cancer via phosphorylating EGFR

sBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides

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