Ferromagnetic Micropallets for Magnetic Capture of Single Adherent Cells

Gunn, Nicholas M.; Chang, Ruth; Westerhof, Trisha M.; Li, G.P.; Bachman, Mark; Nelson, Edward L.

doi:10.1021/la101960v

Cited by 12 publications

(23 citation statements)

References 22 publications

(56 reference statements)

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“…Sacrificial layers provides batch release of multiple microparts, however selectivity of microparts is relatively low compared to other serial release methods. To handle cell-laden planar microparts, fluid flow ejected from a glass capillary [12], contact manipulation by a glass capillary and a microtweezer [12,14,15,32], by a pipette [18], and magnetic actuation [24] are employed.…”

Section: Release and Handling Methodsmentioning

confidence: 99%

“…The layer is often designed to be enduring enough for handling by externals forces, such as fluidic forces [12], and micromanipulators [15]. Typical materials selected for the core layers are glass [12], parylene [13][14][15][16], polystyrene [22,[25][26][27], photoresist such as SU-8 [18-23, 50, 58] and 1002F [22,24,27,28,30,34,35], 1009F epoxy resin [22], poly(lactic-co-glycolic acid) (PLGA) [32], PDMS [52], poly(ethylene glycol) (PEG) hydrogel [54], iron oxide nanoparticles [24,26], and metals such as copper (Cu), nickel (Ni) [49,55], and gold (Au) [49,50,55].…”

Section: Core Layermentioning

confidence: 99%

“…The laser is useful for releasing target micropart due to its high spatial resolution. Figure 10.7i shows magnetic collection of laser released micropart [24]. The micropart is magnetized by iron oxide nanoparticles embedded in the core layer.…”

Section: Handlingmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

Yoshida

Serien

Tomoike

et al. 2015

Hyper Bio Assembler for 3D Cellular Systems

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This chapter focuses on the use of micro-sized cell culture substrates fabricated by micro electro mechanical systems (MEMS) technologies in the field of three-dimensional (3D) cellular tissue constructions. First, we provide a brief overview of MEMS-based tissue engineering methods, and explain why the micro-sized cell-laden substrates are important. Second, we explain the fabrication processes of the micro-sized cell-laden substrates. The fabrication of micro-sized substrates is described by focusing on their materials, structures, cell culture processes, and handling. Third, their applications for single cell handling is described by providing information on observation, collection, and arrangement. Finally, assembly of 3D cellular constructs is explained by pick-and-place assembly, self-assembly, and self-folding methods. Key advantages of the micro-sized substrates are the ability of manipulating various types of adherent cells and also spatial control of cells in 3D cellular tissues. The single cell handling ability facilitates the cell-cell interaction analysis in the field of pharmacology, and 3D cellular assembly ability will open the route for fabrication of spatially-controlled heterogeneous 3D cellular tissues in the regenerative medicine field.

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Section: Release and Handling Methodsmentioning

confidence: 99%

Section: Core Layermentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

Yoshida

Serien

Tomoike

et al. 2015

Hyper Bio Assembler for 3D Cellular Systems

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“…Recently, an array of magnetic microstructures was developed in combination with our previous microarray technology for cell sorting by embedding magnetic nanoparticles within the micropallet array elements. 26,27 The transparent microstructures served as sites for attaching adherent cells. After screening the entire array, the cells of interest could be selectively detached from the array using a pulsed laser and collected against gravity with an external magnet to produce very pure populations of collected cells.…”

Section: Introductionmentioning

confidence: 99%

Isolation and manipulation of living adherent cells by micromolded magnetic rafts

et al. 2011

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A new strategy for magnetically manipulating and isolating adherent cells with extremely high post-collection purity and viability is reported. Micromolded magnetic elements (termed microrafts) were fabricated in an array format and used as culture surfaces and carriers for living, adherent cells. A poly(styrene-co-acrylic acid) polymer containing well dispersed magnetic nanoparticles was developed for creating the microstructures by molding. Nanoparticles of cFe 2 O 3 at concentrations up to 1% wt./wt. could be used to fabricate microrafts that were optically transparent, highly magnetic, biocompatible, and minimally fluorescent. To prevent cellular uptake of nanoparticles from the magnetic polymer, a poly(styrene-coacrylic acid) layer lacking cFe 2 O 3 nanoparticles was placed over the initial magnetic microraft layer to prevent cellular uptake of the cFe 2 O 3 during culture. The microraft surface geometry and physical properties were altered by varying the polymer concentration or layering different polymers during fabrication. Cells plated on the magnetic microrafts were visualized using standard imaging techniques including brightfield, epifluorescence, and confocal microscopy. Magnetic microrafts possessing cells of interest were dislodged from the array and efficiently collected with an external magnet. To demonstrate the feasibility of cell isolation using the magnetic microrafts, a mixed population of wild-type cells and cells stably transfected with a fluorescent protein was plated onto an array. Microrafts possessing single, fluorescent cells were released from the array and magnetically collected. A post-sorting single-cell cloning rate of 92% and a purity of 100% were attained.

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“…16 Needle-based mechanical release involved piercing the elastomeric substrate to dislodge the rafts, and this release process is relatively slow. The objective of this Letter is to investigate the strategy based on ultrasound waves to release pallets for isolation of adherent live cells with high cell survivability and potentially featuring high throughput cell release.…”

mentioning

confidence: 99%

Ultrasound-induced release of micropallets with cells

Guo

Wang

Allbritton

et al. 2012

Applied Physics Letters

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Separation of selected adherent live cells attached on an array of microelements, termed micropallets, from a mixed population is an important process in biomedical research. We demonstrated that adherent cells can be safely, selectively, and rapidly released from the glass substrate together with micropallets using an ultrasound wave. A 3.3-MHz ultrasound transducer was used to release micropallets (500 μm × 500 μm × 300 μm) with attached HeLa cells, and a cell viability of 92% was obtained after ultrasound release. The ultrasound-induced release process was recorded by a high-speed camera, revealing a proximate velocity of ∼0.5 m/s.

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Ferromagnetic Micropallets for Magnetic Capture of Single Adherent Cells

Cited by 12 publications

References 22 publications

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

Isolation and manipulation of living adherent cells by micromolded magnetic rafts

Ultrasound-induced release of micropallets with cells

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