Ferritin protein cage nanoparticles as versatile antigen delivery nanoplatforms for dendritic cell (DC)-based vaccine development

Han, Jae‐A; Kang, Young Ji; Shin, Changsik; Ra, Jae-Sun; Shin, Hong Ju; Hong, Sung You; Do, Yoonkyung; Kang, Sebyung

doi:10.1016/j.nano.2013.11.003

Cited by 91 publications

(59 citation statements)

References 35 publications

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“…DC-based vaccine development has been a promising approach to direct antigen-specific adaptive immunity in vivo [64,65]. Han et al [66] used ferritin nanoparticles to display different ovalbumin antigens. The ovalbumin antigenic peptides OT-1 (Oval-bumin 257–264 ) [67] and OT-2 (Ovalbumin 323–339 ) [67] were genetically introduced either onto the exterior surface or into the interior cavity of ferritin, and the nanoparticles were effectively delivered to DCs and processed within endosomes, followed by successful induction of antigen-specific CD8 + or CD4 + T cells.…”

Section: Ferritin Nanoparticles Used In Vaccine Developmentmentioning

confidence: 99%

Functional ferritin nanoparticles for biomedical applications

Wang

Gao

Zhang

et al. 2017

Front. Chem. Sci. Eng.

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Ferritin, a major iron storage protein with a hollow interior cavity, has been reported recently to play many important roles in biomedical and bioengineering applications. Owing to the unique architecture and surface properties, ferritin nanoparticles offer favorable characteristics and can be either genetically or chemically modified to impart functionalities to their surfaces, and therapeutics or probes can be encapsulated in their interiors by controlled and reversible assembly/disassembly. There has been an outburst of interest regarding the employment of functional ferritin nanoparticles in nanomedicine. This review will highlight the recent advances in ferritin nanoparticles for drug delivery, bioassay, and molecular imaging with a particular focus on their biomedical applications.

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Section: Ferritin Nanoparticles Used In Vaccine Developmentmentioning

confidence: 99%

Functional ferritin nanoparticles for biomedical applications

Wang

Gao

Zhang

et al. 2017

Front. Chem. Sci. Eng.

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“…Following this approach, APO was synthetized in a controlled manner in terms of quantity and location of substituent. By selecting the site for protein insertion, it is possible to expose the antigens on the outer surface [77][78] or both inner and outer [85]. From a technological point of view, new vaccines were characterized by using PCS ant TEM to prove spherical shape and presence of the exposed antigen.…”

Section: A Look Over Cancer Other Possible Applications Of Apo As Ddsmentioning

confidence: 99%

Protein cage nanostructure as drug delivery system: magnifying glass on apoferritin

Belletti

Pederzoli

Forni

et al. 2016

Expert Opinion on Drug Delivery

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New frontiers in nanomedicine are moving towards the research of new biomaterials. Apoferritin (APO), is a uniform regular self-assemblies nano-sized protein with excellent biocompatibility and a unique structure that affords it the ability to stabilize small active molecules in its inner core. Areas covered: APO can be loaded by applying a passive process (mainly used for ions and metals) or by a unique formulative approach based on disassemby/reassembly process. In this article, we aim to organize the experimental evidence provided by a number of studies on the loading, release and targeting. Attention is initially focused on the most investigated antineoplastic drug and contrast agents up to the most recent application in gene therapy. Expert opinion: Various preclinical studies have demonstrated that APO improved the potency and selectivity of some chemotherapeutics. However, in order to translate the use of APO into therapy, some issues must be solved, especially regarding the reproducibility of the loading protocol used, the optimization of nanocarrier characterization, detailed understanding of the final structure of loaded APO, and the real mechanism and timing of drug release.

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“…10 Further, a ferritin heavy chain (FTH) was used as a nanoplatform for antigen delivery to develop a dendritic cell (DC)-based vaccine. 11 Moreover, certain self-assembling peptides act as effective adjuvants that stimulate the induction of potent local IgA antibody responses. 12 Therefore, we considered it important to determine whether multifunctional ferritin cage nanostructures can be used to enhance the induction of salivary IgA antibody to protect against caries.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Self-assembling anticaries mucosal vaccine containing ferritin cage nanostructure and glucan-binding region ofS. mutansglucosyltransferase effectively prevents caries formation in rodents

Cao

et al. 2017

Human Vaccines & Immunotherapeutics

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Anticaries protein vaccines that induce a mucosal immune response are not effective. Therefore, development of effective and convenient anticaries vaccines is a priority of dental research. Here we generated self-assembling nanoparticles by linking the glucan-binding region of Streptococcus mutans glucosyltransferase (GLU) to the N-terminal domain of ferritin to determine whether these novel nanoparticles enhanced the immunogenicity of an anticaries protein vaccine against GLU in rodents. We constructed the expression plasmid pET28a-GLU-FTH and purified the proteins from bacteria using size-exclusion chromatography. BALB/c mice were used to evaluate the ability of GLU-ferritin (GLU-FTH) nanoparticles to induce GLU-specific mucosal and systemic responses. The protective efficiency of GLU-FTH nanoparticles was compared with that of GLU alone or a mixture of GLU and poly(I:C) after administering an intranasal infusion to Wistar rats. The phagocytosis and maturation of dendritic cells (DCs) exposed in vitro to the nanoparticles were assessed using flow cytometry. The GLU-FTH nanoparticle vaccine elicited significantly higher levels of GLU-specific antibodies compared with GLU or a mixture of GLU and poly(I:C). Immunization with GLU-FTH achieved lower caries scores compared with those of the other vaccines. Administration of GLU-FTH nanoparticles enhanced phagocytosis by DCs and their maturation. Thus, self-assembling GLU-FTH is a highly effective anticaries mucosal vaccine that enhanced antibody production and inhibited S. mutans infection in rodents.

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Ferritin protein cage nanoparticles as versatile antigen delivery nanoplatforms for dendritic cell (DC)-based vaccine development

Cited by 91 publications

References 35 publications

Functional ferritin nanoparticles for biomedical applications

Functional ferritin nanoparticles for biomedical applications

Protein cage nanostructure as drug delivery system: magnifying glass on apoferritin

RETRACTED ARTICLE: Self-assembling anticaries mucosal vaccine containing ferritin cage nanostructure and glucan-binding region ofS. mutansglucosyltransferase effectively prevents caries formation in rodents

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