Ferritin gene transcription is regulated by iron in soybean cell cultures.

Lescure, Anne‐Marie; Proudhon, Dominique; Pesey, Hélène; Ragland, Maria; Theil, Elizabeth C.; Briat, Jean‐François

doi:10.1073/pnas.88.18.8222

Cited by 171 publications

(179 citation statements)

References 51 publications

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“…In line with this, ferritin mRNAs are also strongly accumulated in response to iron overload in vascular plants (Arnaud et al, 2006;Lescure et al, 1991;Petit et al, 2001b).…”

Section: Discussionmentioning

confidence: 50%

Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo‐oxidative stress in response to iron availability in Chlamydomonas reinhardtii

et al. 2008

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SummaryFerritin is a key player in the iron homeostasis due to its ability to store large quantities of iron. Chlamydomonas reinhardtii contains two nuclear genes for ferritin (ferr1 and ferr2) that are induced when Chlamydomonas cells are shifted to iron-deficient conditions. In response to the reduced iron availability, degradation of photosystem I (PSI) and remodeling of its light-harvesting complex occur. This active PSI degradation slows down under photo-autotrophic conditions where photosynthesis is indispensable. We observed a strong induction of ferritin correlated with the degree of PSI degradation during iron deficiency. The PSI level can be restored to normal within 24 h after iron repletion at the expense of the accumulated ferritin, indicating that the ferritin-stored iron allows fast adjustment of the photosynthetic apparatus with respect to iron availability. RNAi strains that are significantly reduced in the amount of ferritin show a striking delay in the degradation of PSI under iron deficiency. Furthermore, these strains are more susceptible to photo-oxidative stress under high-light conditions. We conclude that (i) ferritin is used to buffer the iron released by degradation of the photosynthetic complexes, (ii) the physiological status of the cell determines the strategy used to overcome the impact of iron deficiency, (iii) the availability of ferritin is important for rapid degradation of PSI under iron deficiency, and (iv) ferritin plays a protective role under photo-oxidative stress conditions.

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“…In line with this, ferritin mRNAs are also strongly accumulated in response to iron overload in vascular plants (Arnaud et al, 2006;Lescure et al, 1991;Petit et al, 2001b).…”

Section: Discussionmentioning

confidence: 50%

Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo‐oxidative stress in response to iron availability in Chlamydomonas reinhardtii

et al. 2008

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“…It has been demonstrated that ferritin plays a crucial role in iron storage in higher plants [35][36][37] and unicellular microalgae. 19,[38][39][40] On the other hand, ferritin contribution to iron storage in macroalgae or seaweeds is explored only recently, 24) although a ferritin gene is described as a stress-induced gene in a green seaweed Ulva sp.…”

Section: Unpublished Data)mentioning

confidence: 99%

The iron content and ferritin contribution in fresh, dried, and toasted nori, Pyropia yezoensis

Masuda

Yamamoto

Toyohara

2015

Bioscience, Biotechnology, and Biochemistry

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Iron is one of the essential trace elements for humans. In this study, the iron contents in fresh, dried, and toasted nori (Pyropia yezoensis) were analyzed. The mean iron content of fresh, dried, and toasted nori were 19.0, 22.6, and 26.2 mg/100 g (dry weight), respectively. These values were superior to other food of plant origin. Furthermore, most of the iron in nori was maintained during processing, such as washing, drying, and toasting. Then, the form of iron in fresh, dried, and toasted nori was analyzed. As a result, an iron storage protein ferritin contributed to iron storage in raw and dried nori, although the precise rate of its contribution is yet to be determined, while ferritin protein cage was degraded in the toasted nori. It is the first report that verified the ferritin contribution to iron storage in such edible macroalgae with commercial importance.

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“…In contrast, PCs are a family of enzymatically synthesized Cys-rich peptides. The induction of PC synthesis is based on the posttranscriptional activation of preformed PC synthase and is not under transcriptional control.Several heavy metal-inducible genes have been reported in plants (Hagen et al, 1988;Lescure et al, 1991;Berna and Bernier, 1999), but surprisingly, little is known about the transcriptional regulation of gene expression in response to heavy metals. In particular, the cis-acting elements conferring heavy metal responsiveness are poorly characterized, and heavy metalspecific responsive promoters have not yet been reported in higher plants.…”

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confidence: 99%

Characterization of a Novel Plant Promoter Specifically Induced by Heavy Metal and Identification of the Promoter Regions Conferring Heavy Metal Responsiveness

Zhang

Chai

2006

Plant Physiology

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The bean (Phaseolus vulgaris) stress-related gene number 2 (PvSR2) gene responds to heavy metals but not to other forms of environmental stresses. To elucidate its heavy metal-regulatory mechanism at the transcriptional level, we isolated and characterized the promoter region (21623/148) of PvSR2. Deletions from the 5# end revealed that a sequence between 2222 and 2147 relative to the transcriptional start site was sufficient for heavy metal-specific induction of the promoter region of PvSR2. Detailed analysis of this 76-bp fragment indicated that heavy metal-responsive elements were localized in two regions (2222/2188 and 2187/2147), each of which could separately confer heavy metal-responsive expression on the b-glucuronidase gene in the context of a minimal cauliflower mosaic virus 35S promoter. Region I (2222/2188) contains a motif (metalregulatory element-like sequence) similar to the consensus metal-regulatory element of the animal metallothionein gene, and mutation of this motif eliminated the heavy metal-inducible function of region I. Region II (2187/2147) had no similarity to previously identified cis-acting elements involved in heavy metal induction, suggesting the presence of a novel heavy metalresponsive element. Transformed tobacco (Nicotiana tabacum) seedlings expressing b-glucuronidase under control of the PvSR2 promoter region (2687/148) showed heavy metal-specific responsive activity that depended on the type and concentration of the heavy metal and the type of organ. These findings further our understanding of the regulation of PvSR2 expression and provide a new heavy-metal-inducible promoter system in transgenic plants.In plants, a number of heavy metals are essential for normal growth as cofactors and as structural and catalytic components of proteins and enzymes. These micronutrients, as well as nonessential heavy metals such as cadmium, are toxic at high levels. Plants have evolved a suite of mechanisms that control and respond to the uptake and accumulation of both essential and nonessential heavy metals. These mechanisms include the chelation and sequestration of heavy metals by particular ligands including small peptides, organic acids, and amino acids, which bind free metal ions. They contribute to metal detoxification by buffering cytosolic metal ions (Clemens, 2001). The two best-characterized heavy metal-binding ligands in plant cells are the phytochelatins (PCs) and metallothioneins (MTs; for review, see Cobbett and Goldsborough, 2002). MTs are Cys-rich polypeptides encoded by a family of genes. In contrast, PCs are a family of enzymatically synthesized Cys-rich peptides. The induction of PC synthesis is based on the posttranscriptional activation of preformed PC synthase and is not under transcriptional control.Several heavy metal-inducible genes have been reported in plants (Hagen et al., 1988;Lescure et al., 1991;Berna and Bernier, 1999), but surprisingly, little is known about the transcriptional regulation of gene expression in response to heavy metals. In particular, the cis-...

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Ferritin gene transcription is regulated by iron in soybean cell cultures.

Cited by 171 publications

References 51 publications

Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo‐oxidative stress in response to iron availability in Chlamydomonas reinhardtii

Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo‐oxidative stress in response to iron availability in Chlamydomonas reinhardtii

The iron content and ferritin contribution in fresh, dried, and toasted nori, Pyropia yezoensis

Characterization of a Novel Plant Promoter Specifically Induced by Heavy Metal and Identification of the Promoter Regions Conferring Heavy Metal Responsiveness

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