Ferritin acts as the most abundant binding protein for snowdrop lectin in the midgut of rice brown planthoppers (Nilaparvata lugens)

Du, Junzheng; Foissac, Xavier; Carss, A. L.; Gatehouse, Angharad M. R.; Gatehouse, John A.

doi:10.1016/s0965-1748(99)00130-7

Cited by 63 publications

(49 citation statements)

References 25 publications

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“…Initial research on insect ferritins began with the purification of ferritin subunits by biochemical methods, obtaining amino acid sequence, and lastly cloning and characterization of the cDNA encoding the ferritin subunit (Dunkov et al, 1995;Nichol and Locke, 1999;Du et al, 2000;Kim et al, 2001). In this study, a differentially expressed cDNA fragment (P311) was identified by RFDD-PCR technique following induction by dexamethasone.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti (Dunkov et al, 1995;Pham et al, 2000) and G. mellonela (Kim et al, 2001) also lack a glycosylation site. However, the H chain subunit of D. melanogaster (Lind et al, 1998), C. ethlius (Nichol and Locke, 1999), N. lugens (Du et al, 2000), B. mori (Nichol et al, 2002), and the L chain subunit of M. sexta (Pham et al, 1996), C.…”

Section: November 2005mentioning

confidence: 99%

“…ethlius (Nichol and Locke, 1999), and N. lugens (Du et al, 2000) have functional glycosylation sites. Glycosylation of secreted ferritin may increase its stability and assist in intracellular targeting and receptor binding (Nichol and Locke, 1999).…”

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

“…The IRE is conserved among many insects and has a consensus structure of a six-nucleotide loop (CAG-UGN) followed by two 5-bp stem segments that are separated by a single C bulge (Pham et al, 1996;Kim et al, 2001;Charlesworth et al, 1997;Du et al, 2000;Kopáek et al, 2003). There are, however, some ferritin subunits without IRE.…”

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

“…No IRE was reported in yeast, plant, and bacteria (Rothenberger et al, 1990), in yolk of Lymnaea stagnalis (snail) (von Darl et al, 1994), and in Schistosoma mansoni (Schussler et al, 1996). There was likewise no IRE detected in the 5′ UTR of fersub2 from N. lugens and Du et al (2000) suggested that the library may have a truncated 5′ end, which might explain the absence of the IRE. Georgieva et al (1999) reported that Drosophila H chain homologs represented at least four classes of mRNA of various lengths, two of which contain IREs and two of which lack IREs.…”

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

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Sequencing and characterization of a cDNA encoding a ferritin subunit of Colorado potato beetle,Leptinotarsa decemlineata

Qiu

Gao

Clark

2005

Arch. Insect Biochem. Physiol.

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A differentially expressed cDNA fragment (P311) from Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), was identified by restriction fragment differential display-polymerase chain reaction (RFDD-PCR) technique, and showed a strong similarity to ferritin heavy chain subunits of other organisms. Based on P311, we constructed specific primers and obtained a 840-bp cDNA fragment spanning the open reading frame of CPB ferritin subunit using the rapid amplification of cDNA ends (RACE) technique. The sequence encodes 213 amino acid residues, including a 19 amino acid signal peptide. The sequence has a conserved cysteine in the N-terminus and has the seven conserved residues that comprise the ferroxidase center, which is the feature of heavy chain ferritins of vertebrates. The CPB ferritin subunit has high amino acid sequence identity with the Apriona germari (69.3%), Galleria mellonela (54.5%), Manduca sexta (54.0%), Drosophila melanogaster (53.2%), Calpodes ethlius (51.4%), and Nilaparvata lugens (47.6%) but lower identity with the Anopheles gambiae (38.7%) and Aedes aegypti (37.8%). Using Northern blot analysis, the subunit mRNA was identified from fat body and midgut of 4th instars with much higher mRNA levels found in midgut than that in fat body (2.5-fold). Nevertheless, only the levels of mRNA in fat body was induced by dexamethasone (1.5-fold).

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Section: Discussionmentioning

confidence: 99%

Section: November 2005mentioning

confidence: 99%

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

See 3 more Smart Citations

Sequencing and characterization of a cDNA encoding a ferritin subunit of Colorado potato beetle,Leptinotarsa decemlineata

Qiu

Gao

Clark

2005

Arch. Insect Biochem. Physiol.

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Engineering Resistance to Insect Pests

Ferry

Edwards

Mulligan

et al. 2004

Handbook of Plant Biotechnology

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With a projected increase in world population to 10 billion over the next four decades, an immediate priority for agriculture is to achieve maximum production of food and other products in a manner which is environmentally sustainable and cost effective. Despite the synthesis of improved pesticides, and integrated pest management strategies, yield losses due to insects have actually increased slightly for most crops over the last two decades. The concept of utilising a transgenic approach to host plant resistance was realised in the mid 1990s with the commercial introduction of genetically modified crops expressing genes encoding the entomocidal d‐endotoxin from Bacillus thuringiensis ( Bt ). More recently this strategy has been extended to include the pyramiding (stacking) of genes encoding different Bt toxins for greater levels of pest control. Although not as yet a commercial reality, other strategies based on the use of plant derived genes (enzyme inhibitors, lectins) and those from animal sources, including insects (biotin‐binding proteins, neurohormones, enzyme inhibitors), are being developed. The use of fusion proteins to increase the spectrum and durability of resistance is also actively being pursued. However, if transgenic insect‐resistant crops are to play a useful role in crop protection, it is apparent that they must be compatible with the other components of integrated pest management (IPM). The current chapter addresses the role of insect‐resistant transgenic crops in agriculture and discusses both their current status and future developments. Given the importance of natural enemies in controlling pest populations, the potential impact of such crops on predators and parasitoids is also addressed.

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Cloning and expression of 32 kDa ferritin from Galleria mellonella

Kim

Lee

Seol

et al. 2002

Arch Insect Biochem Physiol

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We have sequenced a cDNA clone encoding 32-kDa ferritin subunit in the Wax Moth, Galleria mellonella. The 32-kDa ferritin subunit cDNA was obtained from PCR using identical primer designed from highly conserved regions of insect ferritins. RACE PCR was used to obtain the complete protein coding sequence. The 32-kDa ferritin subunit encoded a 232 amino acid polypeptide, containing a 19 leader peptide. The iron-responsive element (IRE) sequence with a predicted stem-loop structure was present in the 5'-untranslated region of the wax moth 32-kDa ferritin subunit mRNA. The 32-kDa sequence alignment had 78 and 69% identity with Manduca sexta and Calpodes ethlius (G), respectively. The G. mellonella ferritin subunits showed minimal identity with each other (19%). The glycosylation site (Asn-X-Ser/Thr) was found in the 32-kDa subunit but not in the 26-kDa subunit. Northern blot analysis showed that the mRNA expression of the 32-kDa ferritin was detected in the fat body and midgut. The fat body expression increased after 6 h and the mRNA in midgut dramatically increased about 3-fold the expression level at 12 h after iron feeding. Western blot revealed that a protein level of the 32-kDa subunit is abundant in midgut after 12 and 24 h iron feeding.

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Ferritin acts as the most abundant binding protein for snowdrop lectin in the midgut of rice brown planthoppers (Nilaparvata lugens)

Cited by 63 publications

References 25 publications

Sequencing and characterization of a cDNA encoding a ferritin subunit of Colorado potato beetle,Leptinotarsa decemlineata

Sequencing and characterization of a cDNA encoding a ferritin subunit of Colorado potato beetle,Leptinotarsa decemlineata

Engineering Resistance to Insect Pests

Cloning and expression of 32 kDa ferritin from Galleria mellonella

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