Ferrihydrite nanoparticles insights: Structural characterization, lactate dehydrogenase binding and virtual screening assay

Chilom, Claudia G.; Sandu, Nicoleta; Bǎlǎșoiu, M.; Yaroslavtsev, R. N.; Stolyar, S. V.; Rogachev, Andrey

doi:10.1016/j.ijbiomac.2020.08.242

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…The obtained particles have been characterized earlier by means of atomic force microscopy (AFM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and small-angle neutron scattering methods (SANS) [12,13]. The results obtained in previous studies using Scanning Electron Microscopy (SEM) showed that samples investigation present normal size distribution particles with 6.4 nm mean value and 0.94 standard deviation for the Fh-NPs sample, 10.5 nm and 1.65 in the case of Co-Fh-NPs particles and 9.5 nm and 1.47 standard deviation for Cu-Fh-NPs [13].…”

Section: Methodsmentioning

confidence: 99%

“…Ferrihydrite (Fh), (Fe 3+ ) 2 O 3 •0.5H 2 O, an iron oxide, can be synthesized [10,11], as NPs with structural features and properties that can be explored in biophysical and medical applications. One of the directions of research in biophysics is the interaction with biological structures such as proteins [12,13], liposomes and natural membranes [14], and cells [15]. In the medical field, the magnetic properties of the iron nanoparticles, including ferrihydrite nanoparticles (Fh-NPs), have been exploited in the field of the immunoassay [16], magnetic resonance imaging (MRI) [17], and magnetic particle imaging (MPI) for noninvasive imaging of inflammation processes [18], drug delivery [19], and hyperthermia [20].…”

Section: Introductionmentioning

confidence: 99%

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Interactions of Chemically Synthesized Ferrihydrite Nanoparticles with Human Serum Transferrin: Insights from Fluorescence Spectroscopic Studies

Chilom

Sandu

Iftimie

et al. 2021

IJMS

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Human serum transferrin (HST) is a glycoprotein involved in iron transport that may be a candidate for functionalized nanoparticles to bind and target cancer cells. In this study, the effects of the simple and doped with cobalt (Co) and copper (Cu) ferrihydrite nanoparticles (Fh-NPs, Cu-Fh-NPs, and Co-Fh-NPs) were studied by spectroscopic and molecular approaches. Fluorescence spectroscopy revealed a static quenching mechanism for all three types of Fh-NPs. All Fh-NPs interacted with HST with low affinity, and the binding was driven by hydrogen bonding and van der Waals forces for simple Fh-NPs and by hydrophobic interactions for Cu-Fh-NPs and Co-Fh-NPs binding, respectively. Of all samples, simple Fh-NPs bound the most to the HST binding site. Fluorescence resonance energy transfer (FRET) allowed the efficient determination of the energy transfer between HST and NPs and the distance at which the transfer takes place and confirmed the mechanism of quenching. The denaturation of the HST is an endothermic process, both in the case of apo HST and HST in the presence of the three types of Fh-NPs. Molecular docking studies revealed that Fh binds with a low affinity to HST (Ka = 9.17 × 103 M−1) in accord with the fluorescence results, where the interaction between simple Fh-NPs and HST was described by a binding constant of 9.54 × 103 M−1.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interactions of Chemically Synthesized Ferrihydrite Nanoparticles with Human Serum Transferrin: Insights from Fluorescence Spectroscopic Studies

Chilom

Sandu

Iftimie

et al. 2021

IJMS

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“…In recent years, magnetic nanoparticles based on iron oxides have attracted interest in practical applications in the fields of biotechnology and biomedicine. − Surface-modified magnetic nanoparticles can be used for selective isolation of certain biomolecules, such as nucleic acids and proteins. The method, commonly known as “magnetic separation”, has gained popularity in performing tests for diagnostic purposes. , …”

Section: Introductionmentioning

confidence: 99%

Manifestation of Stoichiometry Deviation in Silica-Coated Magnetite Nanoparticles

et al. 2022

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Iron oxide nanoparticles were synthesized by the coprecipitation method. Two varying Fe 3 O 4 /tetraethoxysilane ratios were used for silanization: 1:1.3 and 1:4.5. The samples were investigated using transmission electron microscopy, ferromagnetic resonance, IR spectroscopy, and magnetometry. Magnetic measurements have shown that the magnetite core in nanoparticles has a higher magnetization than stoichiometric magnetite nanoparticles of the same size. The increased magnetization was caused by the deviation of the magnetite stoichiometry due to the interaction with the silicate coating. The blocking temperature distribution was determined from the temperature dependence of the coercive force and from the ZFC/FC dependencies. Nanoparticles with a thicker shell have shown greater efficiency in DNA isolation.

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“…8 Because of its large specific surface area and diverse functional groups, ferrihydrite has a strong adsorption effect for heavy metals. 9,10 Metastable ferrihydrite can transform into more stable iron oxides, like goethite and hematite. 11,12 The transformation rate and products of ferrihydrite can be affected by pH values, foreign impurities, temperature, and organic matters.…”

Section: Introductionmentioning

confidence: 99%

Insights on Pb(ii) retention and immobilization by ferrihydrite in the presence of Al(iii) and oxalic acid

Ariya

et al. 2022

Environ. Sci.: Nano

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Ferrihydrite nanoparticles insights: Structural characterization, lactate dehydrogenase binding and virtual screening assay

Cited by 10 publications

References 39 publications

Interactions of Chemically Synthesized Ferrihydrite Nanoparticles with Human Serum Transferrin: Insights from Fluorescence Spectroscopic Studies

Interactions of Chemically Synthesized Ferrihydrite Nanoparticles with Human Serum Transferrin: Insights from Fluorescence Spectroscopic Studies

Manifestation of Stoichiometry Deviation in Silica-Coated Magnetite Nanoparticles

Insights on Pb(ii) retention and immobilization by ferrihydrite in the presence of Al(iii) and oxalic acid

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