Ferredoxins Containing One [3Fe–4S] Cluster.Desulfovibrio GigasFerredoxinII– Solution Structure

Abstract

Functional Class Occurrence Biological Function Amino Acid Sequence Information Protein Production, Purification and Molecular Characterization Metal Content and Cluster Type Spectroscopy NMR Solution Structure of Native D . Gigas Fd II Functional Aspects Structural Cluster Interconversions: Synthesis of Heterometal Clusters Acknowledgements Related Articles

Isolation and Characterization of the Small Subunit of the Uptake Hydrogenase from the Cyanobacterium Nostoc punctiforme

“…The protein presents two discernible absorption bands in the 300 -500-nm region with maxima at ϳ340 and 420 nm, respectively, as well as a broad shoulder in the 500 -600-nm region. The shape and location of these bands are typical of broad ligand-to-metal charge transfer in iron-sulfur proteins such as ferredoxins (29,30) and indicated that iron-sulfur clusters had been successfully incorporated into f-HupS. This is further substantiated by the 420/315 nm absorbance ratio of 0.43, which is comparable with the corresponding ratio 0.68 found in ferredoxin II from Desulfovibrio gigas (29).…”

“…The shape and location of these bands are typical of broad ligand-to-metal charge transfer in iron-sulfur proteins such as ferredoxins (29,30) and indicated that iron-sulfur clusters had been successfully incorporated into f-HupS. This is further substantiated by the 420/315 nm absorbance ratio of 0.43, which is comparable with the corresponding ratio 0.68 found in ferredoxin II from Desulfovibrio gigas (29). In contrast, the often used 420/280 nm ratio is not a useful measure of purity due to the presence of NusA, 4 D. Camsund and P. Lindblad, personal communication.…”