Fermented Rosa Roxburghii Tratt Juice Alleviates High-Fat Diet-Induced Hyperlipidemia in Rats by Modulating Gut Microbiota and Metabolites

Abstract

Hyperlipidemia endangers human health and has become a significant public health problem. This study aimed to investigate the mechanism of the hypolipidemic effects of Fermented Rosa roxburghii Tratt juice (FRRT) on hyperlipidemic rats and a new hypolipidemic intervention strategy was disclosed. The study revealed 12 weeks FRRT treatment significantly decreased the body weight, total cholesterol (TC), triacylglycerol (TG), low-density lipoprotein cholesterol (LDL-c), while high-density lipoprotein cholesterol (HDL-c) increased. We integrated the 16S rDNA sequencing and metabolomic profiling to evaluate the changes in the gut microbiota and metabolites. Significant changes in microbial composition accompanied marked changes in 56 feces metabolites. The results showed that FRRT could decrease the ratio of Firmicutes to Bacteroidetes, while increase the abundance of some bacterial genera (Prevotella, Paraprevotellaceae_Prevotella, Ruminococcus, Oscillospira). Metabolomics analysis displayed that the metabolisms of bile acid, amino acid and lipid were significantly affected by FRRT. Correlation analysis suggest that the reductions in serum lipids by FRRT are associated with the gut microbial community and their associated metabolites (amino acid metabolites, bile acid metabolites, and lipid metabolites). This study confirmed FRRT could be used as a new dietary and therapeutic strategy to dyslipidemia by improving the gut microbiota dysbiosis, metabolomic disorders and regulating the dyslipidemia. Our study also extended the understanding of the relationship between gut microbiota, metabolites, and lipid-lowering functions.

Blueberry and Blackberry Anthocyanins Ameliorate Metabolic Syndrome by Modulating Gut Microbiota and Short-Chain Fatty Acids Metabolism in High-Fat Diet-Fed C57BL/6J Mice

“…Similar situations also occurred in Allobaculum; in the present study, a positive correlation was found between the abundance of Allobaculum and metabolic parameters, while in some animal and human studies, Allobaculum was approved negatively with insulin resistance, inflammation, dyslipidemia, and obesity . Furthermore, bacteria like Oscillospira and Prevotella , which showed increased abundance in the HVA and HRA groups, have been associated with beneficial effects on lipid metabolism and liver damage, including the reduction of TC, TG, LDL-C, ALT, and AST levels, as well as the increase of HDL-C levels, which is consistent with our results. , These findings indicated that the changes observed in the abundance of specific bacterial taxa in our study could potentially contribute to the beneficial effects of VA and RA supplementation on obesity, liver damage, glucose tolerance, insulin resistance, lipid profile, and inflammation in HFD-fed mice.…”

“…72 Furthermore, bacteria like Oscillospira and Prevotella, which showed increased abundance in the HVA and HRA groups, have been associated with beneficial effects on lipid metabolism and liver damage, including the reduction of TC, TG, LDL-C, ALT, and AST levels, as well as the increase of HDL-C levels, which is consistent with our results. 73,74 These findings indicated that the changes observed in the abundance of specific bacterial taxa in our study could potentially contribute to the beneficial effects of VA and RA supplementation on obesity, liver damage, glucose tolerance, insulin resistance, lipid profile, and inflammation in HFD-fed mice.…”

Unlocking the potential of Rosa roxburghii Tratt polyphenol: a novel approach to treating acute lung injury from a perspective of the lung-gut axis

“…Phytochemical analyses confirmed that RRT contains organic acids, flavonoids, polyphenols and other natural active ingredients, with functional activities incomparable to other fruits ( Wang et al, 2021 ). RRT exhibits a diversity of biological characteristics against immune disorder, inflammatory disease and tumors, and can also regulate gut microbiota dysfunction caused by hyperlipidemia and diabetes ( Wang et al, 2020 ; Ji et al, 2022 ). RRT polyphenol (RRTP), including phenolic acids, coumarins, lignans, tannins, and flavonoids, is one of the most important active components of RRT.…”

Microbial Fermentation Enhances the Effect of Black Tea on Hyperlipidemia by Mediating Bile Acid Metabolism and Remodeling Intestinal Microbes

“…Bile acids have even been suggested as treatment options for liver illness and obesity [19]. In addition, bile acids interact with gut microbes, which have been reported to be tightly connected to hyperlipidemia, and may be a new target for the therapeutic treatment of hyperlipidemia [20,21]. Wu et al indicated that gut microbes are essential for berberine to ameliorate hyperlipidemia [22].…”