Fermented milks with specific Lactobacillus spp. with potential cardioprotective effects

Abstract

In vitro and in vivo studies have reported the potential cardioprotective effects of fermented milks (FM). The aim of the present study was to evaluate the inhibitory activities of angiotensin converting enzyme (ACE), thrombin enzyme (TI) and micellar solubility of cholesterol of FM after 24 and 48 h of fermentation with Limosilactobacillus fermentum (J20, J23, J28 and J38), Lactiplantibacillus plantarum (J25) or Lactiplantibacillus pentosus (J34 and J37) exposed to simulated gastrointestinal digestion. Results showed that FM with J20 and J23 at 48 h of fermentation presented significantly ( p < 0.05) higher degree of hydrolysis than other FM, and were not significantly different ( p > 0.05) between them. Conversely, peptide relative abundance was significantly ( p < 0.05) higher in FM with J20 than FM with J23. Moreover, IC 50 (protein concentration necessary to inhibit enzyme activity by 50%) for ACE inhibition were 0.33 and 0.5 mg/mL for FM with J20 and J23, respectively. For TI inhibition, the IC 50 were 0.3 and 0.24 mg/mL for FM with J20 and J23, respectively. Results exhibited 51 and 74% inhibition of micellar solubility cholesterol for FM with J20 and J23, respectively. Therefore, these results showed that not only peptide abundance, but also specific peptides might be responsible for these potential cardioprotective effects.

Identification of angiotensin‐converting enzyme inhibitory peptides from peanut meal (Arachis hypogaea Linn) fermented by Lactobacillus pentosus using MALDI‐TOF–MS and LC–MS/MS

“…Studies have shown that microbial fermentation can influence the protein molecular structure, and various microbes can produce different enzyme systems and functional factors (Hu et al, 2023;Zambrano-Cervantes et al, 2023). L. pentosus are premium lactic acid bacteria that can ferment pentose sugars to produce high levels of lactic acid; this creates a fermentation microenvironment conducive to their growth (Garcia-Gonzalez et al, 2022;Zhang et al, 2023).…”