Fermentative production of ribonucleotides from whey by Kluyveromyces marxianus: effect of temperature and pH

Abstract

Ribonucleotides have shown many promising applications in food and pharmaceutical industries. The aim of the present study was to produce ribonucleotides (RNA) by Kluyveromyces marxianus ATCC 8,554 utilizing cheese whey, a dairy industry waste, as a main substrate under batch fermentation conditions. The effects of temperature, pH, aeration rate, agitation and initial cellular concentration were studied simultaneously through factorial design for RNA, biomass production and lactose consumption. The maximum RNA production (28.66 mg/g of dry biomass) was observed at temperature 30°C, pH 5.0 and 1 g/l of initial cellular concentration after 2 h of fermentation. Agitation and aeration rate did not influence on RNA concentration (p >0.05). Maximum lactose consumption (98.7%) and biomass production (6.0 g/l) was observed after 12 h of incubation. This study proves that cheese whey can be used as an adequate medium for RNA production by K. marxianus under the optimized conditions at industrial scale.

Optimization of selective production media for enhanced production of xylanases in submerged fermentation by Thielaviopsis basicola MTCC 1467 using L16 orthogonal array

“…To economize the any metabolite production from microbial systems, selection of appropriate carbon and nitrogen source is pivotal (Sreenivas Rao et al 2008;Suresh et al 2011;Húngaro et al 2011). Fungi are the most potent xylanase producers, as they secrete high level of enzymes than those of yeast and bacteria (Qinnghe et al 2004).…”

Genome Sequence of Candida tropicalis no. 121, Used for RNA Production

“…121 was a high-yield strain used for RNA production in our laboratory and Nanjing BioTogether Co. Ltd. Under optimal conditions, the maximum RNA and dry cell weight (DCW) concentrations were >2.5 and 15 g liter -1 ( 5 , 6 ), higher than those for Kluyveromyces marxianus ( 7 ). At the present, the genome sequence of C. tropicalis MYA-3404 is available at the National Center for Biotechnology Information (NCBI) ( 8 ).…”

Rheological and structural characterization of succinoglycan obtained by bioconversion of the agroindustrial residue deproteinized whey

“…It has in its composition lactose (70∼80%), soluble proteins (8∼14%), minerals (12∼15%), lactic acid (0.8∼12%) and lipids (1∼7%), corresponding to 90% of the milk volume and, due to the high carbohydrate content, it can be considered a rich and easy to obtain culture medium (Li, Ding, Chen, Shi, & Wang, 2020). World whey production is estimated at more than 108 tons per year (Húngaro, Calil, Ferreira, Chandel, & Da Silva, 2013). According to Embrapa, in 2020, the cheese industry in Brazil absorbed approximately 8.7 billion liters of milk (Embrapa, 2021).…”