Fermentative production of H2 from different concentrations of galactose by the new isolate Clostridium beijerinckii Br21

Fonseca, Bruna Constante; Guazzaroni, María‐Eugenia; Reginatto, Valéria

doi:10.1016/j.ijhydene.2016.09.110

Cited by 26 publications

(24 citation statements)

References 48 publications

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“…The H 2 ‐producing strain C. beijerinckii Br21 was isolated from an upflow sludge blanket bioreactor (UASB) applied to treat sugarcane vinasse, as described by Fonseca et al C. beijerinckii Br21 was maintained and cultivated in culture medium, called CH medium, at initial pH 6.5 (±0.3). The CH medium consisted of glucose (15.0 g/L), yeast extract (1.0 g/L), Na 2 HPO 4 (5.0 g/L), KH 2 PO 4 (1.0 g/L), NaCl (1.0 g/L), MgSO 4 .7H 2 O (0.1 g/L), FeSO 4 (0.025 g/L), and a trace element solution (0.002 L/L) containing H 3 BO 3 (2.86 g/L), MnSO 4 .4H 2 O (2.03 g/L), and FeCl 3 (0.1 g/L).…”

Section: Methodsmentioning

confidence: 99%

“…The hydrogen concentration in the bioreactor headspace was analyzed on a GC 2014 (Shimadzu, Japan) equipped with a thermal conductivity detector (TCD), according to Fonseca et al…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of acetic, butyric, and lactic acids, as well as of ethanol, butanol, and acetone were analyzed by high performance liquid chromatography (HPLC) conducted on a Shimadzu (Japan) instrument …”

Section: Methodsmentioning

confidence: 99%

“…In our laboratory, the strain Clostridium beijerinckii Br21 (National Center for Biotechnology Information‐NCBI GenBank accession number KT626859) has been recently isolated from an anaerobic sludge employed to treat sugarcane vinasse. Preliminary studies revealed that this strain produces H 2 from many monosaccharides, especially galactose, glucose, and mannose …”

Section: Introductionmentioning

confidence: 99%

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Impact of glucose concentration on productivity and yield of hydrogen production by the new isolate Clostridium beijerinckii Br21

Fonseca

Schmidell²,

Reginatto

2018

Can J Chem Eng

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This paper presents a detailed analysis of the batch kinetic fermentative assays data used to evaluate how glucose concentration influences fermentative H 2 production, including the non-dissociated acid concentration threshold, with the new isolate Clostridium beijerinckii Br21. Fermentative batch assays for hydrogen (H 2 ) production were conducted with initial glucose concentrations of 5.2, 27.2, 54.4, 97.2, or 154.4 mmol/L without pH control. The dry cell mass (X), glucose (S), and product (H 2 ) concentrations and the pH were monitored for 60 h. Increasing initial glucose concentration raised the maximum specific H 2 production rate to values as high as 1.19 mmol H 2 /mg X Á h. However, the H 2 production yield (Y H2/S ) decreased from 2.23 to 0.2 mmol H 2 /mmol glucose. The initial glucose concentration that provided the best compromise between Y H2/S and H 2 productivity was 37 mmol/L (6.7 g/L). The lactic acid, acetic acid, and butyric acid concentrations enhanced at higher initial glucose concentrations, leading to the largest pH decrease. Lower pH favoured non-dissociated acids, which switched the Clostridium metabolism to solventogenesis. The threshold acid concentrations for C. beijerinckii Br21 solventogenesis were non-dissociated butyric acid at 8 mmol/L and total non-dissociated acetic and butyric acids at 13 mmol/L. Glucose supply and pH control in continuous or fed-batch biorreactors could culminate in higher H 2 productivity and yield by C. beijerinckii Br21.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The hydrogen concentration in the bioreactor headspace was analyzed on a GC 2014 (Shimadzu, Japan) equipped with a thermal conductivity detector (TCD), according to Fonseca et al…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of glucose concentration on productivity and yield of hydrogen production by the new isolate Clostridium beijerinckii Br21

Fonseca

Schmidell²,

Reginatto

2018

Can J Chem Eng

Self Cite

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“…The H 2 -producing C. beijerinckii Br21, which can produce H 2 from D-galactose, the main algae biomass monosaccharide, was employed. This microorganism was cultivated as described by FONSECA et al [23]. The culture was prepared 24 h before the inoculum was cultivated, and the culture with 0.1 optical density at 600 nm was added to the hydrolysate.…”

Section: Fermentative H2 Production Assaymentioning

confidence: 99%

Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production

et al. 2019

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Due to its high carbohydrate content, algae biomass can be employed as feedstock to produce hydrogen (H2) by fermentation. However, to make the carbohydrates entrapped within the cell wall more bioavailable, algae biomass must be treated before fermentation. We submitted Kappaphyccus alvarezzi macroalgae biomass to autoclave (at 120 °C and 1 atm for 6 h) treatment and/or enzymatic (Celluclast® and/or a recombinant βglucosidase) hydrolysis, to break down complex carbohydrates into available sugars that were used to produce H2 by fermentation. Macroalgae biomass treated with Celluclast®+β-glucosidase and with combined thermal treatment and enzymatic hydrolysis reached very similar TRS productivities, 0.24 and 0.22 g of TRS/L.h, respectively. The enzymatically treated biomass was employed as feedstock to produce H2 by Clostridium beijerinckii Br21, which afforded high yield: 21.3 mmol of H2/g of dry algae biomass. Hence, treatment with Celluclast® and recombinant β-glucosidase provided macroalgae biomass for enhanced bioconversion to H2 by C. beijerinckii Br21.

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Use of Algae Biomass Obtained by Single-Step Mild Acid Hydrolysis in Hydrogen Production by the β-Glucosidase-Producing Clostridium beijerinckii Br21

Fonseca

Dalbelo

Gelli

et al. 2018

Waste Biomass Valor

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Fermentative production of H2 from different concentrations of galactose by the new isolate Clostridium beijerinckii Br21

Cited by 26 publications

References 48 publications

Impact of glucose concentration on productivity and yield of hydrogen production by the new isolate Clostridium beijerinckii Br21

Impact of glucose concentration on productivity and yield of hydrogen production by the new isolate Clostridium beijerinckii Br21

Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production

Use of Algae Biomass Obtained by Single-Step Mild Acid Hydrolysis in Hydrogen Production by the β-Glucosidase-Producing Clostridium beijerinckii Br21

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