Fermentative production of cycloheximide by <i>Streptomyces griseus</i> and <i>Streptomyces noursei</i>

Abou‐Zeid, A. A.; El-Sherbini, S. H.

doi:10.1002/jctb.2720240413

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…The S. nodosus subnatant induced the biosensor equally strong as amphotericin B ( Figure 5 ) and had no effect on growth, while the S. noursei subnatant heavily impaired growth and therefore no luminescence signal could be detected. Since no interference of pure nystatin with the growth of B. subtilis was observed ( Figures 5C , 6A ), the observed growth impairment in the presence of S. noursei is most likely due to the production of another antibacterial compound by this Streptomyces species ( Abou-Zeid and El-Sherbini, 1974 ; Wu et al, 2009 ).…”

Section: Resultsmentioning

confidence: 92%

Amphotericin B Specifically Induces the Two-Component System LnrJK: Development of a Novel Whole-Cell Biosensor for the Detection of Amphotericin-Like Polyenes

et al. 2020

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The rise of drug-resistant fungal pathogens urges for the development of new tools for the discovery of novel antifungal compounds. Polyene antibiotics are potent agents against fungal infections in humans and animals. They inhibit the growth of fungal cells by binding to sterols in the cytoplasmic membrane that subsequently causes pore formation and eventually results in cell death. Many polyenes are produced by Streptomycetes and released into the soil environment, where they can then target fungal hyphae. While not antibacterial, these compounds could nevertheless be also perceived by bacteria sharing the same habitat and serve as signaling molecules. We therefore addressed the question of how polyenes such as amphotericin B are perceived by the soil bacterium, Bacillus subtilis. Global transcriptional profiling identified a very narrow and specific response, primarily resulting in strong upregulation of the lnrLMN operon, encoding an ABC transporter previously associated with linearmycin resistance. Its strong and specific induction prompted a detailed analysis of the lnrL promoter element and its regulation. We demonstrate that the amphotericin response strictly depends on the two-component system LnrJK and that the target of LnrKdependent gene regulation, the lnrLMN operon, negatively affects LnrJK-dependent signal transduction. Based on this knowledge, we developed a novel whole-cell biosensor, based on a P lnrL-lux fusion reporter construct in a lnrLMN deletion mutant background. This highly sensitive and dynamic biosensor is ready to be applied for the discovery or characterization of novel amphotericin-like polyenes, hopefully helping to increase the repertoire of antimycotic and antiparasitic polyenes available to treat human and animal infections.

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Section: Resultsmentioning

confidence: 92%

Amphotericin B Specifically Induces the Two-Component System LnrJK: Development of a Novel Whole-Cell Biosensor for the Detection of Amphotericin-Like Polyenes

et al. 2020

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“…To nail down the mechanism of protection, we performed experiments with the antifungal compound cycloheximide (CH), which can be produced in soil by bacteria of the genus Streptomyces ( Abou-Zeid and El-Sherbini, 1975 ; Kominek, 1975a , b ; Dykstra and Wang, 1990 ). The analyses indicated that Burkholderia terrae BS001 indeed can provide protection, potentially via shielding/sorption or detoxification effects, from CH to several of the fungi, at different levels of CH.…”

Section: Discussionmentioning

confidence: 99%

Burkholderia terrae BS001 migrates proficiently with diverse fungal hosts through soil and provides protection from antifungal agents

2014

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Soil bacteria can benefit from co-occurring soil fungi in respect of the acquisition of carbonaceous nutrients released by fungal hyphae and the access to novel territories in soil. Here, we investigated the capacity of the mycosphere-isolated bacterium Burkholderia terrae BS001 to comigrate through soil along with hyphae of the soil fungi Trichoderma asperellum, Rhizoctonia solani, Fusarium oxysporum, F. oxysporum pv lini, Coniochaeta ligniaria, Phanerochaete velutina, and Phallus impudicus. We used Lyophyllum sp. strain Karsten as the reference migration-inciting fungus. Bacterial migration through presterilized soil on the extending fungal hyphae was detected with six of the seven test fungi, with only Phallus impudicus not showing any bacterial transport. Much like with Lyophyllum sp. strain Karsten, intermediate (106–108 CFU g-1 dry soil) to high (>108 CFU g-1 dry soil) strain BS001 cell population sizes were found at the hyphal migration fronts of four fungi, i.e., T. asperellum, Rhizoctonia solani, F. oxysporum and F. oxysporum pv lini, whereas for two fungi, Coniochaeta ligniaria and Phanerochaete velutina, the migration responses were retarded and population sizes were lower (103–106 CFU g-1 dry soil). Consistent with previous data obtained with the reference fungus, migration with the migration-inciting fungi occurred only in the direction of the hyphal growth front. Remarkably, Burkholderia terrae BS001 provided protection from several antifungal agents to the canonical host Lyophyllum sp. strain Karsten. Specifically, this host was protected from Pseudomonas fluorescens strain CHA0 metabolites, as well as from the anti-fungal agent cycloheximide. Similar protection by strain BS001was observed for T. asperellum, and, to a lower extent, F. oxysporum and Rhizoctonia solani. The protective effect may be related to the consistent occurrence of biofilm-like cell layers or agglomerates at the surfaces of the protected fungi. The current study represents the first report of protection of soil fungi against antagonistic agents present in the soil provided by fungal-associated Burkholderia terrae cells.

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“…Secondary metabolites of microorganisms, such as Streptomyces species produce a plethora of compounds, such as polyketides (Caffrey, 2012), peptides (Agrawal et al, 2017), and polyketide-peptide hybrids (Du et al, 2003). Most of these compounds have been characterized with different biological activities, including antibacterial (Procópio et al, 2012;Jang et al, 2013;Sivalingam et al, 2019), antifungal (Abou-Zeid andEl-Sherbini, 1974;Qi et al, 2019), anticancer (Nachtigall et al, 2011;Tan et al, 2015), and immune suppressive ones (Sehgal, 1998;Bolourian and Mojtahedi, 2018). Furthermore, microalgae (Barkia et al, 2019), diatoms (Tolomio et al, 2002), and thermophilic Cyanobacteria detected in peloids (Marcolongo et al, 2006;Kim Tiam et al, 2019;Zampieri et al, 2020) also produce compounds such as sulfoglycolipids, glycoglycerolipids known for their bioactivity.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting for Microorganisms in Peloids—Extreme Environment Known for Its Healing Properties

et al. 2022

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Seawater is an environment in which numerous microorganisms have evolved, some with a great potential for biotechnology. In recent years, many scientists have moved away from the assumption that the origin of life was in pools of water, and instead propose that life on Earth probably originated in accumulations of warm, nutrient-rich mud. This mud, also called peloid is a rich source of organisms that, due to their adaptation to this unique environment, produce a wide variety of primary and secondary metabolites with numerous and diverse activities, including anti-cancer, anti-inflammatory, antiviral, and immunomodulatory ones. In this research, two questions were addressed using collected samples of a peloid with demonstrated healing properties. Firstly biodiversity in this ecological niche was explored in order to assess microbial communities present and secondly natural products were screened for in order to assess whether predicted activities could be linked to healing properties of the peloid. The use of peloids in medical therapy dates back to ancient times. Abiotic components such as clay and mineral water are believed to be the main contributors of the healing properties of natural peloids. The places where peloids are usually found are characteristically shallow and enclosed lagoons. The constant UV exposure and increased salt concentration classify peloid as an extreme environment. The spectrum of relief’s peloid therapy is claimed to provide ranges from purely cosmetic and skin-related to musculoskeletal and immunological problems. These claims can hardly be supported by mineral content and heat-retaining properties alone. However, organic compounds from present microorganisms as well as secondary metabolites could help explain the observed range of health benefits. The fact that the relationship between the therapeutic activity of peloids and their composition besides mineral and physicochemical properties has not been extensively studied indicates untapped biotechnological potential.

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Fermentative production of cycloheximide by Streptomyces griseus and Streptomyces noursei

Cited by 3 publications

References 17 publications

Amphotericin B Specifically Induces the Two-Component System LnrJK: Development of a Novel Whole-Cell Biosensor for the Detection of Amphotericin-Like Polyenes

Amphotericin B Specifically Induces the Two-Component System LnrJK: Development of a Novel Whole-Cell Biosensor for the Detection of Amphotericin-Like Polyenes

Burkholderia terrae BS001 migrates proficiently with diverse fungal hosts through soil and provides protection from antifungal agents

Bioprospecting for Microorganisms in Peloids—Extreme Environment Known for Its Healing Properties

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