Fermentative hydrogen production by microbial consortium

Maintinguer, Sandra Imaculada; Fernandes, Bruna Soares; Duarte, Iolanda Cristina Silveira; Saavedra, Nora Kátia; Adorno, Maria Ângela Tallarico; Varesche, Maria Bernadete Amâncio

doi:10.1016/j.ijhydene.2008.06.053

Cited by 101 publications

(86 citation statements)

References 29 publications

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“…The sludge was subjected to 10 min of heat treatment at 90 ºC to inactivate the H 2 consumers and harvest endospore-forming anaerobic bacteria (Maintinguer et al, 2008 .…”

Section: Inoculum and Fermentation Mediummentioning

confidence: 99%

“…× 0.25 μm film thickness) (Maintinguer et al, 2008). The biogas hydrogen content was measured using a gas chromatograph (GC-2010, Shimadzu, Japan) with a thermal conductivity detector (TCD) with argon as the carrier gas, and the column was packed with Supelco Carboxen 1010 Plot (30 m × 0.53 mm i.d.)…”

Section: Chemical Analysismentioning

confidence: 99%

“…Furthermore, the heat treatment of the inoculum contributes to the selection of hydrogen-producing bacteria by preventing the growth of hydrogen-consuming methanogens (Maintinguer et al, 2008).…”

Section: Composition Of Soluble Productsmentioning

confidence: 99%

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Biohydrogen production from cassava wastewater in an anaerobic fluidized bed reactor

Amorim

Alves

Martins

et al. 2014

Braz. J. Chem. Eng.

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-The effect of hydraulic retention time (HRT) and organic loading rate (OLR) on biological hydrogen production was assessed using an anaerobic fluidized bed reactor fed with cassava wastewater. The HRT of this reactor ranged from 8 to 1 h (28 to 161 kg COD/m 3 -d). The inoculum was obtained from a facultative pond sludge derived from swine wastewater treatment. The effluent pH was approximately 5.00, while the influent chemical oxygen demand (COD) measured 4000 mg COD/L. The hydrogen yield production increased from 0.13 to 1.91 mol H 2 /mol glucose as the HRT decreased from 8 to 2 h. The hydrogen production rate significantly increased from 0.20 to 2.04 L/h/L when the HRT decreased from 8 to 1 h. The main soluble metabolites were ethanol (1.87-100%), acetic acid (0.00-84.80%), butyric acid (0.00-66.78%) and propionic acid (0.00-50.14%). Overall, we conclude that the best hydrogen yield production was obtained at an HRT of 2 h.

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“…The sludge was subjected to 10 min of heat treatment at 90 ºC to inactivate the H 2 consumers and harvest endospore-forming anaerobic bacteria (Maintinguer et al, 2008 .…”

Section: Inoculum and Fermentation Mediummentioning

confidence: 99%

Section: Chemical Analysismentioning

confidence: 99%

See 1 more Smart Citation

Biohydrogen production from cassava wastewater in an anaerobic fluidized bed reactor

Amorim

Alves

Martins

et al. 2014

Braz. J. Chem. Eng.

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“…After image analysis, bands were excised from the gel with sterile blade, eluted with 20 μL of nanopure water at 4 °C overnight, and then amplified with the same primers (without the CG clamp in the forward primer) (Maintinguer et al, 2008). The following program of amplification was performed: initial denaturation at 94 °C for 5 minutes; 35 cycles at 94°C for 1 minute, 49ºC for 1 minute and 72ºC for 2 minutes; final extension at 72°C for 5 minutes.…”

Section: Sequencing and Statistical Analysismentioning

confidence: 99%

Bacterial degradation of dissolved organic matter released by Planktothrix agardhii (Cyanobacteria)

et al. 2017

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Although Planktothrix agardhii often produces toxic blooms in eutrophic water bodies around the world, little is known about the fate of the organic matter released by these abundant Cyanobacteria. Thus, this study focused in estimating the bacterial consumption of the DOC and DON (dissolved organic carbon and dissolved organic nitrogen, respectively) produced by axenic P. agardhii cultures and identifying some of the bacterial OTUs (operational taxonomic units) involved in the process. Both P. agardhii and bacterial inocula were sampled from the eutrophic Barra Bonita Reservoir (SP, Brazil). Two distinct carbon degradation phases were observed: during the first three days, higher degradation coefficients were calculated, which were followed by a slower degradation phase. The maximum value observed for particulate bacterial carbon (POC) was 11.9 mg L -1 , which consisted of 62.5% of the total available DOC, and its mineralization coefficient was 0.477 day -1 (t½ = 1.45 days). A similar pattern of degradation was observed for DON, although the coefficients were slightly different. Changes in the OTUs patterns were observed during the different steps of the degradation. The main OTUs were related to the classes Alphaproteobacteria (8 OTUs), Betaproteobacteria (2 OTUs) and Gammaproteobacteria (3 OTUs). The genus Acinetobacter was the only identified organism that occurred during the whole process. Bacterial richness was higher at the slower degradation phase, which could be related to the small amounts of DOM (dissolved organic matter) available, particularly carbon. The kinetics of the bacterial degradation of P. agardhii-originated DOM suggests minimal loss of DOM from the Barra Bonita reservoir.Keywords: dissolved organic matter, bacterial community, DGGE, degradation. Degradação bacteriana da matéria orgânica dissolvida liberada porPlanktothrix agardhii (Cyanobacteria) ResumoEmbora Planktothrix agardhii frequentemente forme florações tóxicas em corpos d'água pelo mundo, pouco ainda se sabe sobre o destino da matéria orgânica liberada por essa abundante Cyanobacteria. Assim, este estudo foi focado na estimativa do consumo bacteriano do carbono orgânico dissolvido (DOC) e nitrogênio orgânico dissolvido (DON) produzido por culturas axênicas de P. agardhii e identificação de algumas das unidades taxonômicas operacionais (OTUs) bacterianas envolvidas no processo. Ambos a linhagem de P. agardhii e o inóculo bacteriano foram amostrados do reservatório eutrófico de Barra Bonita (SP, Brasil). Foram observadas duas fases distintas da degradação do DOC: durante os três primeiros dias, coeficientes mais altos de degradação foram calculados, que foram então seguidos por uma fase mais lenta da degradação do carbono. O valor máximo calculado para o carbono bacteriano particulado (POC) foi de 11,9 mgL -1 , o que equivale a aproximadamente 62,5% do DOC disponível para consumo, e o seu coeficiente de mineralização foi de 0,477 dia -1 (t 1/2 = 1,45 dias). Um padrão similar de degradação foi observado para DON, embora os c...

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“…These configurations can be used to investigate nutritional requirements (Maintinguer et al, 2008). The denitrification process with complex carbohydrates is being evaluated in batch reactors because the particulate organic matter that is present in these systems can difficult the operation in other configurations, such as with starch (Iamamoto, 2006).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the microbial diversity of denitrifying bacteria in batch reactor

Maintinguer

Sakamoto

Adorno

et al. 2013

Braz. J. Chem. Eng.

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-Microbial communities in an industrial activated sludge plant may contribute to the denitrification process, but the information on the microorganisms present in denitrifying reactors is still scarce. Removal of inorganic nitrogen compounds can be accomplished by the addition of carbon sources to the biological process of denitrification. Ethanol is an economically viable alternative as a carbon source in tropical countries like Brazil, with large-scale production from sugarcane. This paper reports the successful aplication of activated sludge with nitrate and ethanol in a batch anaerobic reactor. The operation lasted 61.5 h with total consumption of nitrate in 42.5 h, nitrite generation (2.0 mg/L) and ethanol consumption (830.0 mg/L) in 23.5 h. Denitrifying cell counts by the most probable number at the start of the operation were lower than at the end, confirming the ability of the inoculum from activated sludge for the denitrification process. The samples from cell counts were identified as Acidovorax sp., Acinetobacter sp., Comamonas sp. and uncultured bacteria. Therefore, these species may be involved in nitrate reduction and ethanol consumption in the batch reactor.

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Fermentative hydrogen production by microbial consortium

Cited by 101 publications

References 29 publications

Biohydrogen production from cassava wastewater in an anaerobic fluidized bed reactor

Biohydrogen production from cassava wastewater in an anaerobic fluidized bed reactor

Bacterial degradation of dissolved organic matter released by Planktothrix agardhii (Cyanobacteria)

Evaluation of the microbial diversity of denitrifying bacteria in batch reactor

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